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. 1984 Apr 11;12(7):3115–3126. doi: 10.1093/nar/12.7.3115

Comparative structural analysis of eubacterial 5S rRNA by oxidation of adenines in the N-1 position.

T Pieler, A Schreiber, V A Erdmann
PMCID: PMC318733  PMID: 6201825

Abstract

Adenines in free 5S rRNA from Escherichia coli, Bacillus stearothermophilus and Thermus thermophilus have been oxidized at their N-1 position using monoperphthalic acid. The determination of the number of adenine 1-N-oxides was on the basis of UV spectroscopic data of the intact molecule. Identification of the most readily accessible nucleotides by sequencing gel analysis reveals that they are located in conserved positions within loops, exposed hairpin loops and single-base bulge loops. Implications for the structure and function of 5S rRNA will be discussed on the basis of this comparative analysis.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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