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. 1984 Apr 11;12(7):3387–3404. doi: 10.1093/nar/12.7.3387

Methylphosphonates as probes of protein-nucleic acid interactions.

S A Noble, E F Fisher, M H Caruthers
PMCID: PMC318753  PMID: 6718253

Abstract

Deoxydinucleoside methylphosphonates were prepared by chemical synthesis and were introduced stereospecifically into the lac operator at two sites. These sites within d(ApApTpTpGpTpGpApGpCpGpGpApTpApApCpApApTpT), segment I, and d(ApApTpTpGpTpTpApTpCpCpGpCpTpCpApCpApApTpT), segment II, are indicated by p. Each segment containing a chiral methylphosphonate was annealed to the complementary unmodified segment. The interactions of these four modified lac operators with lac repressor were analyzed by the nitrocellulose filter binding assay. Introduction of either chiral phosphonate in segment II had little effect on the stability of the repressor-operator complex. When methylphosphonates were introduced into segment I, the affinity of lac repressor for the modified operators was shown to be dependent on the stereochemical configuration of the methylphosphonate.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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