Skip to main content
PMC home page
Nucleic Acids Research logoLink to Nucleic Acids Research
. 1984 May 25;12(10):4071–4081. doi: 10.1093/nar/12.10.4071

Initiation and termination signals for transcription in bacteriophage M13.

M A Smits, J Jansen, R N Konings, J G Schoenmakers
PMCID: PMC318817  PMID: 6328409

Abstract

Transcription of the infrequently expressed phage M13 genome domain, comprising genes III, VI, I and IV, has been studied in detail by hybridization and S1-nuclease mapping studies. The contiguous genes III and VI are transcribed via an 1800 nucleotide-long RNA molecule that is initiated at a promoter which overlaps with the Rho-independent termination signal between genes III and VIII. Its synthesis is terminated at a Rho-dependent terminator in the proximal part of gene I. Transcription of gene I is not mediated by an independent promoter but most probably by read-through of RNA-polymerase through this terminator. Transcription of gene IV is accomplished by synthesis of four distinct RNAs of about 1500 to 1680 nucleotides long which are initiated at a promoter located immediately in front of gene IV. Termination of these transcripts is generated at least four different sites located in tandem within the intergenic region between genes IV and II.

Full text

PDF
4072

Images in this article

4075Image
on p.4075
4076Image
on p.4076
4078Image
on p.4078

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Adler H. I., Fisher W. D., Cohen A., Hardigree A. A. MINIATURE escherichia coli CELLS DEFICIENT IN DNA. Proc Natl Acad Sci U S A. 1967 Feb;57(2):321–326. doi: 10.1073/pnas.57.2.321. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Beck E., Zink B. Nucleotide sequence and genome organisation of filamentous bacteriophages fl and fd. Gene. 1981 Dec;16(1-3):35–58. doi: 10.1016/0378-1119(81)90059-7. [DOI] [PubMed] [Google Scholar]
  3. Bonner W. M., Laskey R. A. A film detection method for tritium-labelled proteins and nucleic acids in polyacrylamide gels. Eur J Biochem. 1974 Jul 1;46(1):83–88. doi: 10.1111/j.1432-1033.1974.tb03599.x. [DOI] [PubMed] [Google Scholar]
  4. Casey J., Davidson N. Rates of formation and thermal stabilities of RNA:DNA and DNA:DNA duplexes at high concentrations of formamide. Nucleic Acids Res. 1977;4(5):1539–1552. doi: 10.1093/nar/4.5.1539. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Cashman J. S., Webster R. E. Bacteriophage f1 infection of Escherichia coli: identification and possible processing of f1-specific mRNAs in vivo. Proc Natl Acad Sci U S A. 1979 Mar;76(3):1169–1173. doi: 10.1073/pnas.76.3.1169. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Cashman J. S., Webster R. E., Steege D. A. Transcription of bacteriophage fl. The major in vivo RNAs. J Biol Chem. 1980 Mar 25;255(6):2554–2562. [PubMed] [Google Scholar]
  7. Das A., Court D., Adhya S. Isolation and characterization of conditional lethal mutants of Escherichia coli defective in transcription termination factor rho. Proc Natl Acad Sci U S A. 1976 Jun;73(6):1959–1963. doi: 10.1073/pnas.73.6.1959. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Donelson J. E., Wu R. Nucleotide sequence analysis of deoxyribonucleic acid. VI. Determination of 3'-terminal dnucleotide sequences of several species of duplex deoxyribonucleic acid using Escherichia coli deoxyribonucleic acid polymerase I. J Biol Chem. 1972 Jul 25;247(14):4654–4660. [PubMed] [Google Scholar]
  9. Dotto G. P., Enea V., Zinder N. D. Functional analysis of bacteriophage f1 intergenic region. Virology. 1981 Oct 30;114(2):463–473. doi: 10.1016/0042-6822(81)90226-9. [DOI] [PubMed] [Google Scholar]
  10. Edens L., Konings R. N., Schoenmakers J. G. Transcription of bacteriophage M13 DNA: existence of promoters directly preceding genes III, VI, and I. J Virol. 1978 Dec;28(3):835–842. doi: 10.1128/jvi.28.3.835-842.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Geider K., Beck E., Schaller H. An RNA transcribed from DNA at the origin of phage fd single strand to replicative form conversion. Proc Natl Acad Sci U S A. 1978 Feb;75(2):645–649. doi: 10.1073/pnas.75.2.645. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Gray C. P., Sommer R., Polke C., Beck E., Schaller H. Structure of the orgin of DNA replication of bacteriophage fd. Proc Natl Acad Sci U S A. 1978 Jan;75(1):50–53. doi: 10.1073/pnas.75.1.50. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Hill D. F., Petersen G. B. Nucleotide sequence of bacteriophage f1 DNA. J Virol. 1982 Oct;44(1):32–46. doi: 10.1128/jvi.44.1.32-46.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Holmes W. M., Platt T., Rosenberg M. Termination of transcription in E. coli. Cell. 1983 Apr;32(4):1029–1032. doi: 10.1016/0092-8674(83)90287-8. [DOI] [PubMed] [Google Scholar]
  15. La Farina M., Model P. Transcription in bacteriophage F1-infected Escherichia coli. I. Translation of the RNA in vitro. Virology. 1978 May 15;86(2):368–375. doi: 10.1016/0042-6822(78)90077-6. [DOI] [PubMed] [Google Scholar]
  16. La Farina M., Model P. Transcription in bacteriophage f1-infected Escherichia coli. Messenger populations in the infected cell. J Mol Biol. 1983 Mar 5;164(3):377–393. doi: 10.1016/0022-2836(83)90057-8. [DOI] [PubMed] [Google Scholar]
  17. Maxam A. M., Gilbert W. Sequencing end-labeled DNA with base-specific chemical cleavages. Methods Enzymol. 1980;65(1):499–560. doi: 10.1016/s0076-6879(80)65059-9. [DOI] [PubMed] [Google Scholar]
  18. Moses P. B., Model P. A rho-dependent transcription termination signal in bacteriophage f1. J Mol Biol. 1984 Jan 5;172(1):1–22. doi: 10.1016/0022-2836(84)90411-x. [DOI] [PubMed] [Google Scholar]
  19. Platt T. Termination of transcription and its regulation in the tryptophan operon of E. coli. Cell. 1981 Apr;24(1):10–23. doi: 10.1016/0092-8674(81)90496-7. [DOI] [PubMed] [Google Scholar]
  20. Pratt D., Tzagoloff H., Beaudoin J. Conditional lethal mutants of the small filamentous coliphage M13. II. Two genes for coat proteins. Virology. 1969 Sep;39(1):42–53. doi: 10.1016/0042-6822(69)90346-8. [DOI] [PubMed] [Google Scholar]
  21. Rivera M. J., Smits M. A., Quint W., Schoenmakers J. G., Konings R. N. Expression of bacteriophage M13 DNA in vivo. Localization of the transcription initiation and termination signal of the mRNA coding for the major capsid protein. Nucleic Acids Res. 1978 Aug;5(8):2895–2912. doi: 10.1093/nar/5.8.2895. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Schaller H., Uhlmann A., Geider K. A DNA fragment from the origin of single-strand to double-strand DNA replication of bacteriophage fd. Proc Natl Acad Sci U S A. 1976 Jan;73(1):49–53. doi: 10.1073/pnas.73.1.49. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Scherer G. E., Walkinshaw M. D., Arnott S. A computer aided oligonucleotide analysis provides a model sequence for RNA polymerase-promoter recognition in E.coli. Nucleic Acids Res. 1978 Oct;5(10):3759–3773. doi: 10.1093/nar/5.10.3759. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Siebenlist U., Simpson R. B., Gilbert W. E. coli RNA polymerase interacts homologously with two different promoters. Cell. 1980 Jun;20(2):269–281. doi: 10.1016/0092-8674(80)90613-3. [DOI] [PubMed] [Google Scholar]
  25. Smits M. A., Schoenmakers J. G., Konings R. N. Expression of bacteriophage M13 DNA in vivo. Isolation, identification and characterization of phage-specific mRNA species. Eur J Biochem. 1980 Nov;112(2):309–321. doi: 10.1111/j.1432-1033.1980.tb07206.x. [DOI] [PubMed] [Google Scholar]
  26. Smits M. A., Simons G., Konings R. N., Schoenmakers J. G. Expression of bacteriophage M13 dna in vivo. I. Synthesis of phage-specific RNA and protein in minicells. Biochim Biophys Acta. 1978 Nov 21;521(1):27–44. doi: 10.1016/0005-2787(78)90246-0. [DOI] [PubMed] [Google Scholar]
  27. Takanami M., Okamoto T., Sugiura M. Termination of RNA transcription on the replicative form DNA of bacteriophage fd. J Mol Biol. 1971 Nov 28;62(1):81–88. doi: 10.1016/0022-2836(71)90132-x. [DOI] [PubMed] [Google Scholar]
  28. van Wezenbeek P. M., Hulsebos T. J., Schoenmakers J. G. Nucleotide sequence of the filamentous bacteriophage M13 DNA genome: comparison with phage fd. Gene. 1980 Oct;11(1-2):129–148. doi: 10.1016/0378-1119(80)90093-1. [DOI] [PubMed] [Google Scholar]

Articles from Nucleic Acids Research are provided here courtesy of Oxford University Press

RESOURCES