Skip to main content
PMC home page
Nucleic Acids Research logoLink to Nucleic Acids Research
. 1984 Jun 25;12(12):5079–5086. doi: 10.1093/nar/12.12.5079

Basepairing of oligonucleotides to the 3' end of 16S ribosomal RNA is not stabilized by ribosomal proteins.

J Van Duin, C J Ravensbergen, J Doornbos
PMCID: PMC318901  PMID: 6739298

Abstract

We have studied the binding of the octanucleotide (5'-3')d(AAGGAGGT) which is fully complementary to the 3' end of 16S ribosomal RNA, to ribosomes and to the isolated target sequence (5'-3') (ACCUCCUUA). The binding constant for 30S or 70S ribosomes is (5 +/- 2) X 10(7) mol-1, whereas the duplex containing the octa- and the nonanucleotide has an association constant of (6 +/- 3) X 10(7) mol-1. The two values are the same within the experimental error. This result suggests that basepairing at the 3' end of 16S rRNA is not stabilized by ribosomal proteins.

Full text

PDF
5083

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Backendorf C., Overbeek G. P., Van Boom J. H., Van Der Marel G., Veeneman G., Van Duin J. Role of 16-S RNA in ribosome messenger recognition. Eur J Biochem. 1980 Sep;110(2):599–604. doi: 10.1111/j.1432-1033.1980.tb04904.x. [DOI] [PubMed] [Google Scholar]
  2. Backendorf C., Ravensbergen C. J., Van der Plas J., van Boom J. H., Veeneman G., Van Duin J. Basepairing potential of the 3' terminus of 16S RNA: dependence on the functional state of the 30S subunit and the presence of protein S21. Nucleic Acids Res. 1981 Mar 25;9(6):1425–1444. doi: 10.1093/nar/9.6.1425. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bear D. G., Ng R., Van Derveer D., Johnson N. P., Thomas G., Schleich T., Noller H. F. Alteration of polynucleotide secondary structure by ribosomal protein S1. Proc Natl Acad Sci U S A. 1976 Jun;73(6):1824–1828. doi: 10.1073/pnas.73.6.1824. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Dunn J. J., Buzash-Pollert E., Studier F. W. Mutations of bacteriophage T7 that affect initiation of synthesis of the gene 0.3 protein. Proc Natl Acad Sci U S A. 1978 Jun;75(6):2741–2745. doi: 10.1073/pnas.75.6.2741. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Eckhardt H., Lührmann R. Blocking of the initiation of protein biosynthesis by a pentanucleotide complementary to the 3' end of Escherichia coli 16 S rRNA. J Biol Chem. 1979 Nov 25;254(22):11185–11188. [PubMed] [Google Scholar]
  6. Hardy S. J. The stoichiometry of the ribosomal proteins of Escherichia coli. Mol Gen Genet. 1975 Oct 3;140(3):253–274. doi: 10.1007/BF00334270. [DOI] [PubMed] [Google Scholar]
  7. Kolb A., Hermoso J. M., Thomas J. O., Szer W. Nucleic acid helix-unwinding properties of ribosomal protein S1 and the role of S1 in mRNA binding to ribosomes. Proc Natl Acad Sci U S A. 1977 Jun;74(6):2379–2383. doi: 10.1073/pnas.74.6.2379. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Shine J., Dalgarno L. The 3'-terminal sequence of Escherichia coli 16S ribosomal RNA: complementarity to nonsense triplets and ribosome binding sites. Proc Natl Acad Sci U S A. 1974 Apr;71(4):1342–1346. doi: 10.1073/pnas.71.4.1342. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Steitz J. A., Jakes K. How ribosomes select initiator regions in mRNA: base pair formation between the 3' terminus of 16S rRNA and the mRNA during initiation of protein synthesis in Escherichia coli. Proc Natl Acad Sci U S A. 1975 Dec;72(12):4734–4738. doi: 10.1073/pnas.72.12.4734. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Steitz J. A., Steege D. A. Characterization of two mRNA-rRNA complexes implicated in the initiation of protein biosynthesis. J Mol Biol. 1977 Aug 25;114(4):545–558. doi: 10.1016/0022-2836(77)90177-2. [DOI] [PubMed] [Google Scholar]
  11. Steitz J. A., Wahba A. J., Laughrea M., Moore P. B. Differential requirements for polypeptide chain initiation complex formation at the three bacteriophage R17 initiator regions. Nucleic Acids Res. 1977 Jan;4(1):1–15. doi: 10.1093/nar/4.1.1. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Taniguchi T., Weissmann C. Inhibition of Qbeta RNA 70S ribosome initiation complex formation by an oligonucleotide complementary to the 3' terminal region of E. coli 16S ribosomal RNA. Nature. 1978 Oct 26;275(5682):770–772. doi: 10.1038/275770a0. [DOI] [PubMed] [Google Scholar]
  13. Voynow P., Kurland C. G. Stoichiometry of the 30S ribosomal proteins of Escherichia coli. Biochemistry. 1971 Feb 2;10(3):517–524. doi: 10.1021/bi00779a026. [DOI] [PubMed] [Google Scholar]
  14. Zamir A., Miskin R., Vogel Z., Elson D. The inactivation and reactivation of Escherichia coli ribosomes. Methods Enzymol. 1974;30:406–426. doi: 10.1016/0076-6879(74)30042-0. [DOI] [PubMed] [Google Scholar]

Articles from Nucleic Acids Research are provided here courtesy of Oxford University Press

RESOURCES