Abstract
The adenovirus 12--simian virus 40 hybrid virus produced neoplastic transformation of chimpanzee skin fibroblasts in vitro. The transformed fibroblasts showed morphological alteration and became permanent lines. The transformed cells contained both adenovirus 12 and simian virus 40 large tumor antigens and were virus producers. However at passage 9, one line (WES) was found to be a nonproducer, producing neither infectious virus nor virus-specific antigen detectable by the complement fixation test. Virus particles were not detected nor could infectious hybrid virus be rescued from this line by cocultivation with Vero cells. The transformed cells formed large cell aggregates and grew in liquid growth medium above an agar base, formed colonies in soft agar, and grew to high saturation densities; the normal chimpanzee skin fibroblasts did not. One transformed WES line produced tumors when transplanted subcutaneously into newborn nude mice, thus providing an important tool for studying tumor immunity in the chimpanzee.
Full text
PDF
Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- BLACK P. H., ROWE W. P. AN ANALYSIS OF SV40-INDUCED TRANSFORMATION OF HAMSTER KIDNEY TISSUE IN VITRO. I. GENERAL CHARACTERISTICS. Proc Natl Acad Sci U S A. 1963 Oct;50:606–613. doi: 10.1073/pnas.50.4.606. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Black P. H., Todaro G. J. In vitro transformation of hamster and human cells with the adeno 7-SV 40 hybrid virus. Proc Natl Acad Sci U S A. 1965 Aug;54(2):374–381. doi: 10.1073/pnas.54.2.374. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Black P. H., White B. J. In vitro transformation by the adenovirus-SV40 hybrid viruses. II. Characteristics of the transformation of hamster cells by the adeno 2-, adeno 3-, and adeno 12-SV40 viruses. J Exp Med. 1967 Apr 1;125(4):629–646. doi: 10.1084/jem.125.4.629. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Gajdusek D. C., Gibbs C. J., Alpers M. Experimental transmission of a Kuru-like syndrome to chimpanzees. Nature. 1966 Feb 19;209(5025):794–796. doi: 10.1038/209794a0. [DOI] [PubMed] [Google Scholar]
- HUEBNER R. J., CHANOCK R. M., RUBIN B. A., CASEY M. J. INDUCTION BY ADENOVIRUS TYPE 7 OF TUMORS IN HAMSTERS HAVING THE ANTIGENIC CHARACTERISTICS OF SV40 VIRUS. Proc Natl Acad Sci U S A. 1964 Dec;52:1333–1340. doi: 10.1073/pnas.52.6.1333. [DOI] [PMC free article] [PubMed] [Google Scholar]
- HUEBNER R. J., ROWE W. P., TURNER H. C., LANE W. T. SPECIFIC ADENOVIRUS COMPLEMENT-FIXING ANTIGENS IN VIRUS-FREE HAMSTER AND RAT TUMORS. Proc Natl Acad Sci U S A. 1963 Aug;50:379–389. doi: 10.1073/pnas.50.2.379. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Rhim J. S., Schell K., Creasy B., Case W. Biological characteristics and viral susceptibility of an African green monkey kidney cell line (Vero). Proc Soc Exp Biol Med. 1969 Nov;132(2):670–678. doi: 10.3181/00379727-132-34285. [DOI] [PubMed] [Google Scholar]
- Schell K., Lane W. T., Casey M. J., Huebner R. J. Potentiation of oncogenicity of adenovirus type 12 grown in African green monkey kidney cell cultures preinfected with SV40 virus. Persistence of both T antigens in the tumors and evidence for possible hybridization. Proc Natl Acad Sci U S A. 1966 Jan;55(1):81–88. doi: 10.1073/pnas.55.1.81. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Steuer A. F., Rhim J. S., Hentosh P. M., Ting R. C. Survival of human cells in the aggregate form: potential index of in vitro cell transformation. J Natl Cancer Inst. 1977 Apr;58(4):917–921. doi: 10.1093/jnci/58.4.917. [DOI] [PubMed] [Google Scholar]