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. 1981 Jan;78(1):653–657. doi: 10.1073/pnas.78.1.653

Immunohistochemical localization of cyclic GMP-dependent protein kinase in mammalian brain.

S M Lohmann, U Walter, P E Miller, P Greengard, P De Camilli
PMCID: PMC319113  PMID: 7017721

Abstract

The distribution of cyclic GMP-dependent protein kinase in rat brain has been studied by an immunological approach involving radioimmunoassay and fluorescence immunohistochemistry. Data obtained by radioimmunoassay indicate that cyclic GMP-dependent protein kinase is 20- to 40-fold more concentrated in cerebellum than in other brain regions. Immunohistochemical experiments demonstrate that the high concentration of immunoreactivity of the protein kinase in cerebellum is attributable to Purkinje cells. Immunoreactivity in these cells is homogeneously distributed throughout the cell (perikarya, dendrites, and axons) with the exception of the nucleus. No other neurons either in the cerebellum or in other brain regions were stained by antiserum to the protein kinase. Immunoreactivity, however, was found throughout the brain on smooth muscle cells of blood vessels.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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