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. 1981 May;78(5):3083–3087. doi: 10.1073/pnas.78.5.3083

Regulation of synthesis of cell-specific ribosomal proteins during differentiation of Dictyostelium discoideum*

Subbanaidu Ramagopal 1,†, Herbert L Ennis 1,‡
PMCID: PMC319504  PMID: 16593020

Abstract

Proteins of ribosomes from various stages of development in Dictyostelium discoideum were analyzed by two-dimensional polyacrylamide gel electrophoresis. Significant changes in protein composition were observed; the data demonstrate that cell differentiation in a eukaryotic system is accompanied by ribosome heterogeneity. Both qualitative and quantitative differences were noted for 12 unique ribosomal proteins between the vegetative amoebae and spores (differentiated cells). Two proteins were specific to ribosomes of amoebae, and three were specific to spores. The others were common to both cells but showed characteristic stoichiometric changes. The appearance and quantitative changes of these proteins were associated with specific stages of cell differentiation and were evident only during the aggregation phase; however, further changes continued through construction of fruiting bodies. As functional mRNAs for all 12 proteins were present in both amoebae and spores, both transcriptional and translational mechanisms apparently regulate the synthesis of the various developmentally controlled ribosomal proteins in the two cell types.

Keywords: slime mold, development, ribosome heterogeneity, ribosomal proteins, translational control

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Alton T. H., Lodish H. F. Developmental changes in messenger RNAs and protein synthesis in Dictyostelium discoideum. Dev Biol. 1977 Oct 1;60(1):180–206. doi: 10.1016/0012-1606(77)90118-x. [DOI] [PubMed] [Google Scholar]
  2. Alton T. H., Lodish H. F. Synthesis of developmentally regulated proteins in Dictyostelium discoideum which are dependent on continued cell-cell interaction. Dev Biol. 1977 Oct 1;60(1):207–216. doi: 10.1016/0012-1606(77)90119-1. [DOI] [PubMed] [Google Scholar]
  3. Boublik M., Ramagopal S. Conformation of ribosomes from the vegetative amoebae and spores of Dictyoistelium discoideum. Mol Gen Genet. 1980;179(3):483–488. doi: 10.1007/BF00271737. [DOI] [PubMed] [Google Scholar]
  4. Brimacombe R., Stöffler G., Wittmann H. G. Ribosome structure. Annu Rev Biochem. 1978;47:217–249. doi: 10.1146/annurev.bi.47.070178.001245. [DOI] [PubMed] [Google Scholar]
  5. Cocucci S. M., Sussman M. RNA in cytoplasmic and nuclear fractions of cellular slime mold amebas. J Cell Biol. 1970 May;45(2):399–407. doi: 10.1083/jcb.45.2.399. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Cox R. A. Structure and function of prokaryotic and eukaryotic ribosomes. Prog Biophys Mol Biol. 1977;32(3):193–231. [PubMed] [Google Scholar]
  7. DeWitt W., Price R. P. Structural changes in ribosomes during development. Biochem Biophys Res Commun. 1974 Feb 4;56(3):593–598. doi: 10.1016/0006-291x(74)90645-7. [DOI] [PubMed] [Google Scholar]
  8. Delaunay J., Mathieu C., Schapira G. Eukaryotic ribosomal proteins. Interspecific and intraspecific comparisons by two-dimensional polyacrylamide-gel electrophoresis. Eur J Biochem. 1972 Dec 18;31(3):561–564. doi: 10.1111/j.1432-1033.1972.tb02565.x. [DOI] [PubMed] [Google Scholar]
  9. Dowbenko D. J., Ennis H. L. Regulation of protein synthesis during spore germination in Dictyostelium discoideum. Proc Natl Acad Sci U S A. 1980 Apr;77(4):1791–1795. doi: 10.1073/pnas.77.4.1791. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Giri J. G., Ennis H. L. Protein and RNA synthesis during spore germination in the cellular slime mold Dictyostelium discoideum. Biochem Biophys Res Commun. 1977 Jul 11;77(1):282–289. doi: 10.1016/s0006-291x(77)80194-0. [DOI] [PubMed] [Google Scholar]
  11. Huynh-Van-Tan, Delaunay J., Schapira G. Eukaryotic ribosomal proteins. Two-dimensional electrophoretic studies. FEBS Lett. 1971 Sep 15;17(1):163–167. doi: 10.1016/0014-5793(71)80588-4. [DOI] [PubMed] [Google Scholar]
  12. Jacobson A., Lodish H. F. Genetic control of development of the cellular slime mold, Dictyostelium discoideum. Annu Rev Genet. 1975;9:145–185. doi: 10.1146/annurev.ge.09.120175.001045. [DOI] [PubMed] [Google Scholar]
  13. Kristiansen K., Krüger A. Ribosomal proteins in growing and starved Tetrahymena pyriformis. Starvation-induced phosphorylation of ribosomal proteins. Biochim Biophys Acta. 1978 Dec 21;521(2):435–451. doi: 10.1016/0005-2787(78)90285-x. [DOI] [PubMed] [Google Scholar]
  14. Ma G. C., Firtel R. A. Regulation of the synthesis of two carbohydrate-binding proteins in Dictyostelium discoideum. J Biol Chem. 1978 Jun 10;253(11):3924–3932. [PubMed] [Google Scholar]
  15. Margolskee J. P., Lodish H. F. The regulation of the synthesis of actin and two other proteins induced early in Dictyostelium discoideum development. Dev Biol. 1980 Jan;74(1):50–64. doi: 10.1016/0012-1606(80)90052-4. [DOI] [PubMed] [Google Scholar]
  16. Mutolo V., Giudice G., Hopps V., Donatuti G. Species specificity of embryonic ribosomal proteins. Biochim Biophys Acta. 1967 Mar 29;138(1):214–217. doi: 10.1016/0005-2787(67)90609-0. [DOI] [PubMed] [Google Scholar]
  17. Ramagopal S., Ennis H. L. Studies on ribosomal proteins in the cellular slime mold Dictyostelium discoideum. Resolution, nomenclature and molecular weights of proteins in the 40-S and 60-S ribosomal subunits. Eur J Biochem. 1980 Apr;105(2):245–258. doi: 10.1111/j.1432-1033.1980.tb04495.x. [DOI] [PubMed] [Google Scholar]
  18. Ramagopal S., Subramanian A. R. Alteration in the acetylation level of ribosomal protein L12 during growth cycle of Escherichia coli. Proc Natl Acad Sci U S A. 1974 May;71(5):2136–2140. doi: 10.1073/pnas.71.5.2136. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Rodgers A. Ribosomal proteins in rapidly growing and non-proliferating mouse cells. Biochim Biophys Acta. 1973 Jan 19;294(1):292–296. [PubMed] [Google Scholar]
  20. Schimke R. T. Control of enzyme levels in mammalian tissues. Adv Enzymol Relat Areas Mol Biol. 1973;37:135–187. doi: 10.1002/9780470122822.ch3. [DOI] [PubMed] [Google Scholar]
  21. Sherton C. C., Wool I. G. A comparison of the proteins of rat skeletal muscle and liver ribosomes by two-dimensional polyacrylamide gel electrophoresis. Observations on the partition of proteins between ribosomal subunits and a description of two acidic proteins in the large subunit. J Biol Chem. 1974 Apr 10;249(7):2258–2267. [PubMed] [Google Scholar]
  22. Sussman R. R. RNA metabolism during cytodifferentiation in the cellular slime mold Polysphondelium pallidum. Biochim Biophys Acta. 1967 Dec 19;149(2):407–421. doi: 10.1016/0005-2787(67)90169-4. [DOI] [PubMed] [Google Scholar]
  23. Wool I. G. The structure and function of eukaryotic ribosomes. Annu Rev Biochem. 1979;48:719–754. doi: 10.1146/annurev.bi.48.070179.003443. [DOI] [PubMed] [Google Scholar]

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