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. 1981 Jun;78(6):3313–3317. doi: 10.1073/pnas.78.6.3313

Expression in Escherichia coli of biologically active enzyme by a DNA sequence coding for the human plasminogen activator urokinase.

B Ratzkin, S G Lee, W J Schrenk, R Roychoudhury, M Chen, T A Hamilton, P P Hung
PMCID: PMC319558  PMID: 7022447

Abstract

We have isolated clones of Escherichia coli strain K-12 that contain a hybrid pBR322 plasmid having a 4.2-kilobase insert of a DNA transcript of the mRNA of human plasminogen activator, urokinase. The bacterially produced enzyme has properties similar to those of urokinase from human fetal kidney cells. Both enzymes occur in discrete forms ranging from 32,000 to 150,000 daltons in size. They react with antibody to purified urokinase from human kidney cells, bind to a benzamidine-Sepharose column, and induce plasminogen-dependent lysis of a fibrin clot.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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