Skip to main content
PMC home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1981 Jul;78(7):4584–4587. doi: 10.1073/pnas.78.7.4584

Platelet factor 4 is chemotactic for neutrophils and monocytes.

T F Deuel, R M Senior, D Chang, G L Griffin, R L Heinrikson, E T Kaiser
PMCID: PMC319837  PMID: 6945600

Abstract

Platelet factor 4 is shown to be a chemotactic protein for human polymorphonuclear leukocytes and monocytes at concentrations found in human serum and reached locally in injured tissue. The maximum chemotactic response to platelet factor 4 nearly equals that achieved with saturating concentrations of the chemotactic activity derived from the fifth component of human complement, C5. Cells desensitized to C5 chemotactic activity retain chemotactic responsiveness to platelet factor 4. Serum contains inhibitory capacity against the chemotactic activity associated with platelet factor 4. Our results suggest that the local release of platelet factor 4 may be an important stimulus attracting inflammatory cells to sites of blood vessel injury.

Full text

PDF
4585

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Braunstein P. W., Cuénoud H. F., Joris I., Majno G. Platelets, fibroblasts, and inflammation: tissue reactions to platelets injected subcutaneously. Am J Pathol. 1980 Apr;99(1):53–66. [PMC free article] [PubMed] [Google Scholar]
  2. Campbell P. B. An improved method for the in vitro evaluation of monocyte leukotaxis. J Lab Clin Med. 1977 Aug;90(2):381–388. [PubMed] [Google Scholar]
  3. Deuel T. F., Keim P. S., Farmer M., Heinrikson R. L. Amino acid sequence of human platelet factor 4. Proc Natl Acad Sci U S A. 1977 Jun;74(6):2256–2258. doi: 10.1073/pnas.74.6.2256. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Fantone J., Senior R. M., Kreutzer D. L., Jones M., Ward P. A. Biochemical quantitation of the chemotactic factor inactivator activity in human serum. J Lab Clin Med. 1979 Jan;93(1):17–24. [PubMed] [Google Scholar]
  5. Fukami M. H., Niewiarowski S., Rucinski B., Salganicoff L. Subcellular localization of human platelet antiheparin proteins. Thromb Res. 1979 Feb-Mar;14(2-3):433–443. doi: 10.1016/0049-3848(79)90252-4. [DOI] [PubMed] [Google Scholar]
  6. Goetzl E. J., Tashjian A. H., Jr, Rubin R. H., Austen K. F. Production of a low molecular weight eosinophil polymorphonuclear leukocyte chemotactic factor by anaplastic squamous cell carcinomas of human lung. J Clin Invest. 1978 Mar;61(3):770–780. doi: 10.1172/JCI108991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Goetzl E. J., Woods J. M., Gorman R. R. Stimulation of human eosinophil and neutrophil polymorphonuclear leukocyte chemotaxis and random migration by 12-L-hydroxy-5,8,10,14-eicosatetraenoic acid. J Clin Invest. 1977 Jan;59(1):179–183. doi: 10.1172/JCI108617. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Hunninghake G. W., Gadek J. E., Fales H. M., Crystal R. G. Human alveolar macrophage-derived chemotactic factor for neutrophils. Stimuli and partial characterization. J Clin Invest. 1980 Sep;66(3):473–483. doi: 10.1172/JCI109878. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Kaplan A. P., Goetzl E. J., Austen K. F. The fibrinolytic pathway of human plasma. II. The generation of chemotactic activity by activation of plasminogen proactivator. J Clin Invest. 1973 Oct;52(10):2591–2595. doi: 10.1172/JCI107451. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Kaplan K. L., Broekman M. J., Chernoff A., Lesznik G. R., Drillings M. Platelet alpha-granule proteins: studies on release and subcellular localization. Blood. 1979 Apr;53(4):604–618. [PubMed] [Google Scholar]
  11. Kay A. B., Pepper D. S., Ewart M. R. Generation of chemotactic activity for leukocytes by the action of thrombin on human fibrinogen. Nat New Biol. 1973 May 9;243(123):56–57. [PubMed] [Google Scholar]
  12. Kay A. B., Pepper D. S., McKenzie R. The identification of fibrinopeptide B as a chemotactic agent derived from human fibrinogen. Br J Haematol. 1974 Aug;27(4):669–677. doi: 10.1111/j.1365-2141.1974.tb06633.x. [DOI] [PubMed] [Google Scholar]
  13. Kay A. B. Studies on eosinophil leucocyte migration. II. Factors specifically chemotactic for eosinophils and neutrophils generated from guinea-pig serum by antigen-antibody complexes. Clin Exp Immunol. 1970 Nov;7(5):723–737. [PMC free article] [PubMed] [Google Scholar]
  14. Kreutzer D. L., Claypool W. D., Jones M. L., Ward P. A. Isolation by hydrophobic chromatography of the chemotactic factor inactivators from human serum. Clin Immunol Immunopathol. 1979 Feb;12(2):162–176. doi: 10.1016/0090-1229(79)90005-9. [DOI] [PubMed] [Google Scholar]
  15. Lachmann P. J., Kay A. B., Thompson R. A. The chemotactic activity for neutrophil and eosinophil leucocytes of the trimolecular complex of the fifth, sixth and seventh components of human complement (C567) prepared in free solution by the 'reactive lysis' procedure. Immunology. 1970 Dec;19(6):895–899. [PMC free article] [PubMed] [Google Scholar]
  16. Merrill W. W., Naegel G. P., Matthay R. A., Reynolds H. Y. Alveolar macrophage-derived chemotactic factor: kinetics of in vitro production and partial characterization. J Clin Invest. 1980 Feb;65(2):268–276. doi: 10.1172/JCI109668. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Mustard J. F., Movat H. Z., Macmorine D. R., Sényi A. Release of permeability factors from the blood platelet. Proc Soc Exp Biol Med. 1965 Aug-Sep;119(4):988–991. doi: 10.3181/00379727-119-30356. [DOI] [PubMed] [Google Scholar]
  18. Nachman R. L., Weksler B., Ferris B. Characterization of human platelet vascular permeability-enhancing activity. J Clin Invest. 1972 Mar;51(3):549–556. doi: 10.1172/JCI106843. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Nachman R. L., Weksler B., Ferris B. Increased vascular permeability produced by human platelet granule cationic extract. J Clin Invest. 1970 Feb;49(2):274–281. doi: 10.1172/JCI106237. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. O'Flaherty J. T., Kreutzer D. L., Showell H. J., Vitkauskas G., Becker E. L., Ward P. A. Selective neutrophil desensitization to chemotactic factors. J Cell Biol. 1979 Mar;80(3):564–572. doi: 10.1083/jcb.80.3.564. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Packham M. A., Nishizawa E. E., Mustard J. F. Response of platelets to tissue injury. Biochem Pharmacol. 1968 Mar;(Suppl):171–184. doi: 10.1016/0006-2952(68)90304-3. [DOI] [PubMed] [Google Scholar]
  22. Pinckard R. N., Halonen M., Palmer J. D., Butler C., Shaw J. O., Henson P. M. Intravascular aggregation and pulmonary sequestration of platelets during IgE-induced systemic anaphylaxis in the rabbit: abrogation of lethal anaphylactic shock by platelet depletion. J Immunol. 1977 Dec;119(6):2185–2193. [PubMed] [Google Scholar]
  23. Postlethwaite A. E., Kang A. H. Collagen-and collagen peptide-induced chemotaxis of human blood monocytes. J Exp Med. 1976 Jun 1;143(6):1299–1307. doi: 10.1084/jem.143.6.1299. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Postlethwaite A. E., Keski-Oja J., Balian G., Kang A. H. Induction of fibroblast chemotaxis by fibronectin. Localization of the chemotactic region to a 140,000-molecular weight non-gelatin-binding fragment. J Exp Med. 1981 Feb 1;153(2):494–499. doi: 10.1084/jem.153.2.494. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Ross R., Glomset J., Kariya B., Harker L. A platelet-dependent serum factor that stimulates the proliferation of arterial smooth muscle cells in vitro. Proc Natl Acad Sci U S A. 1974 Apr;71(4):1207–1210. doi: 10.1073/pnas.71.4.1207. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Ryo R., Proffitt R. T., Deuel T. F. Human platelet factor 4: subcellular localization and characteristics of release from intact platelets. Thromb Res. 1980 Mar 1;17(5):629–644. doi: 10.1016/0049-3848(80)90366-7. [DOI] [PubMed] [Google Scholar]
  27. Schiffmann E., Corcoran B. A., Wahl S. M. N-formylmethionyl peptides as chemoattractants for leucocytes. Proc Natl Acad Sci U S A. 1975 Mar;72(3):1059–1062. doi: 10.1073/pnas.72.3.1059. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Senior R. M., Griffin G. L., Mecham R. P. Chemotactic activity of elastin-derived peptides. J Clin Invest. 1980 Oct;66(4):859–862. doi: 10.1172/JCI109926. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Sobel J. D., Gallin J. I. Polymorphonuclear leukocyte and monocyte chemoattractants produced by human fibroblasts. J Clin Invest. 1979 Apr;63(4):609–618. doi: 10.1172/JCI109343. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Spilbert I., Gallacher A., Mehta J. M., Mandell B. Urate crystal-induced chemotactic factor: isolation and partial characterization. J Clin Invest. 1976 Oct;58(4):815–819. doi: 10.1172/JCI108533. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. Ward P. A. A plasmin-split fragment of C'3 as a new chemotactic factor. J Exp Med. 1967 Aug 1;126(2):189–206. doi: 10.1084/jem.126.2.189. [DOI] [PMC free article] [PubMed] [Google Scholar]
  32. Ward P. A., Cochrane C. G., Muller-Eberhard H. J. Further studies on the chemotactic factor of complement and its formation in vivo. Immunology. 1966 Aug;11(2):141–153. [PMC free article] [PubMed] [Google Scholar]
  33. Ward P. A., Lepow I. H., Newman L. J. Bacterial factors chemotactic for polymorphonuclear leukocytes. Am J Pathol. 1968 Apr;52(4):725–736. [PMC free article] [PubMed] [Google Scholar]
  34. Ward P. A., Newman L. J. A neutrophil chemotactic factor from human C'5. J Immunol. 1969 Jan;102(1):93–99. [PubMed] [Google Scholar]
  35. Ward P. A., Remold H. G., David J. R. Leukotactic factor produced by sensitized lymphocytes. Science. 1969 Mar 7;163(3871):1079–1081. doi: 10.1126/science.163.3871.1079. [DOI] [PubMed] [Google Scholar]
  36. Weiss H. J. Platelet physiology and abnormalities of platelet function (second of two parts). N Engl J Med. 1975 Sep 18;293(12):580–588. doi: 10.1056/NEJM197509182931204. [DOI] [PubMed] [Google Scholar]
  37. Wilkinson P. C., Borel J. F., Stecher-Levin V. J., Sorkin E. Macrophage and neutrophil specific chemotactic factors in serum. Nature. 1969 Apr 19;222(5190):244–247. doi: 10.1038/222244a0. [DOI] [PubMed] [Google Scholar]
  38. Williams L. T., Snyderman R., Pike M. C., Lefkowitz R. J. Specific receptor sites for chemotactic peptides on human polymorphonuclear leukocytes. Proc Natl Acad Sci U S A. 1977 Mar;74(3):1204–1208. doi: 10.1073/pnas.74.3.1204. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES