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. 1981 Feb;78(2):922–925. doi: 10.1073/pnas.78.2.922

Molecular basis of valine resistance in Escherichia coli K-12.

R P Lawther, D H Calhoun, C W Adams, C A Hauser, J Gray, G W Hatfield
PMCID: PMC319916  PMID: 7015336

Abstract

The relationship of valine resistance to the expression of the ilvGEDA operon of Escherichia coli K-12 has been determined. DNA sequence and in vivo protein analyses indicate that in wild-type E. coli K-12 there is a frameshift site within the gene (ilvG) for valine resistance. The ilvG+2096 (formerly designated ilv02096) mutation displaces this frameshift site, resulting in the expression of ilvG and the relief of transcriptional polarity on the distal genes of this operon. Thus, the "ilv0" mutation, which concomitantly confers valine resistance and increased expression of the ilvEDA genes, is, in fact, the "reversion" of a polar site within the first structural gene of the ilvGEDA operon.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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