Skip to main content
PMC home page
Nucleic Acids Research logoLink to Nucleic Acids Research
. 1984 Oct 25;12(20):7771–7785. doi: 10.1093/nar/12.20.7771

Sequences of the coding and flanking regions of the large ribosomal subunit RNA gene of mosquito mitochondria.

C C HsuChen, R M Kotin, D T Dubin
PMCID: PMC320199  PMID: 6093053

Abstract

We have sequenced a 1.6 kbp region of the mosquito (Aedes albopictus) mitochondrial genome containing the large ribosomal subunit ("LSU") RNA gene, and have located the ends of the gene by S1 protection analysis and by comparison with RNA sequences. The gene is preceded by a tRNAval gene and followed by genes for tRNAIeuUAG (rather than tRNAleuUAA, as in mammalian mitochondria) and an extended reading frame homologous to mammalian URF1. It is approximately 1335 residues long and is very low (17%) in G + C. The 5' half is even lower in G + C (9%), and shows little apparent homology to other LSU RNA classes. The 3' half is relatively rich (26%) in G + C and has many stretches of homology to prokaryotic and mammalian mitochondrial LSU RNA.

Full text

PDF
7773

Images in this article

7776Image
on p.7776
7777Image
on p.7777

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Anderson S., Bankier A. T., Barrell B. G., de Bruijn M. H., Coulson A. R., Drouin J., Eperon I. C., Nierlich D. P., Roe B. A., Sanger F. Sequence and organization of the human mitochondrial genome. Nature. 1981 Apr 9;290(5806):457–465. doi: 10.1038/290457a0. [DOI] [PubMed] [Google Scholar]
  2. Anderson S., de Bruijn M. H., Coulson A. R., Eperon I. C., Sanger F., Young I. G. Complete sequence of bovine mitochondrial DNA. Conserved features of the mammalian mitochondrial genome. J Mol Biol. 1982 Apr 25;156(4):683–717. doi: 10.1016/0022-2836(82)90137-1. [DOI] [PubMed] [Google Scholar]
  3. Baer R. J., Dubin D. T. Methylated regions of hamster mitochondrial ribosomal RNA: structural and functional correlates. Nucleic Acids Res. 1981 Jan 24;9(2):323–337. doi: 10.1093/nar/9.2.323. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Bibb M. J., Van Etten R. A., Wright C. T., Walberg M. W., Clayton D. A. Sequence and gene organization of mouse mitochondrial DNA. Cell. 1981 Oct;26(2 Pt 2):167–180. doi: 10.1016/0092-8674(81)90300-7. [DOI] [PubMed] [Google Scholar]
  5. Blanc H., Adams C. W., Wallace D. C. Different nucleotide changes in the large rRNA gene of the mitochondrial DNA confer chloramphenicol resistance on two human cell lines. Nucleic Acids Res. 1981 Nov 11;9(21):5785–5795. doi: 10.1093/nar/9.21.5785. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Branlant C., Krol A., Machatt M. A., Pouyet J., Ebel J. P., Edwards K., Kössel H. Primary and secondary structures of Escherichia coli MRE 600 23S ribosomal RNA. Comparison with models of secondary structure for maize chloroplast 23S rRNA and for large portions of mouse and human 16S mitochondrial rRNAs. Nucleic Acids Res. 1981 Sep 11;9(17):4303–4324. doi: 10.1093/nar/9.17.4303. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Brown T. A., Davies R. W., Ray J. A., Waring R. B., Scazzocchio C. The mitochondnal genome of Aspergillus nidulans contains reading frames homologous to the human URFs 1 and 4. EMBO J. 1983;2(3):427–435. doi: 10.1002/j.1460-2075.1983.tb01440.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Clary D. O., Goddard J. M., Martin S. C., Fauron C. M., Wolstenholme D. R. Drosophila mitochondrial DNA: a novel gene order. Nucleic Acids Res. 1982 Nov 11;10(21):6619–6637. doi: 10.1093/nar/10.21.6619. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Clary D. O., Wahleithner J. A., Wolstenholme D. R. Sequence and arrangement of the genes for cytochrome b, URF1, URF4L, URF4, URF5, URF6 and five tRNAs in Drosophila mitochondrial DNA. Nucleic Acids Res. 1984 May 11;12(9):3747–3762. doi: 10.1093/nar/12.9.3747. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Clary D. O., Wolstenholme D. R. Nucleotide sequence of a segment of Drosophila mitochondrial DNA that contains the genes for cytochrome c oxidase subunits II and III and ATPase subunit 6. Nucleic Acids Res. 1983 Jun 25;11(12):4211–4227. doi: 10.1093/nar/11.12.4211. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Dubin D. T., HsuChen C. C. The 3'-terminal region of mosquito mitochondrial small ribosomal subunit RNA: sequence and localization of methylated residues. Plasmid. 1983 May;9(3):307–320. doi: 10.1016/0147-619x(83)90008-2. [DOI] [PubMed] [Google Scholar]
  12. Dubin D. T. Methylated nucleotide content of mitochondrial ribosomal RNA from hamster cells. J Mol Biol. 1974 Apr 5;84(2):257–273. doi: 10.1016/0022-2836(74)90584-1. [DOI] [PubMed] [Google Scholar]
  13. Dubin D. T., Montoya J., Timko K. D., Attardi G. Sequence analysis and precise mapping of the 3' ends of HeLa cell mitochondrial ribosomal RNAs. J Mol Biol. 1982 May 5;157(1):1–19. doi: 10.1016/0022-2836(82)90510-1. [DOI] [PubMed] [Google Scholar]
  14. Dubin D. T., Timko K. D., Baer R. J. The 3' terminus of the large ribosomal subunit ("17S") RNA from hamster mitochondria is ragged and oligoadenylated. Cell. 1981 Jan;23(1):271–278. doi: 10.1016/0092-8674(81)90291-9. [DOI] [PubMed] [Google Scholar]
  15. Glotz C., Zwieb C., Brimacombe R., Edwards K., Kössel H. Secondary structure of the large subunit ribosomal RNA from Escherichia coli, Zea mays chloroplast, and human and mouse mitochondrial ribosomes. Nucleic Acids Res. 1981 Jul 24;9(14):3287–3306. doi: 10.1093/nar/9.14.3287. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Hanahan D., Meselson M. Plasmid screening at high colony density. Gene. 1980 Jun;10(1):63–67. doi: 10.1016/0378-1119(80)90144-4. [DOI] [PubMed] [Google Scholar]
  17. HsuChen C. C., Cleaves G. R., Dubin D. T. A major lysine tRNA with a CUU anticodon in insect mitochondria. Nucleic Acids Res. 1983 Dec 20;11(24):8659–8662. doi: 10.1093/nar/11.24.8659. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. HsuChen C. C., Dubin D. T. A cluster of four transfer RNA genes in mosquito mitochondrial DNA. Biochem Int. 1984 Mar;8(3):385–391. [PubMed] [Google Scholar]
  19. Köchel H. G., Küntzel H. Mitochondrial L-rRNA from Aspergillus nidulans: potential secondary structure and evolution. Nucleic Acids Res. 1982 Aug 11;10(15):4795–4801. doi: 10.1093/nar/10.15.4795. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Maly P., Brimacombe R. Refined secondary structure models for the 16S and 23S ribosomal RNA of Escherichia coli. Nucleic Acids Res. 1983 Nov 11;11(21):7263–7286. doi: 10.1093/nar/11.21.7263. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Maxam A. M., Gilbert W. Sequencing end-labeled DNA with base-specific chemical cleavages. Methods Enzymol. 1980;65(1):499–560. doi: 10.1016/s0076-6879(80)65059-9. [DOI] [PubMed] [Google Scholar]
  22. Noller H. F., Kop J., Wheaton V., Brosius J., Gutell R. R., Kopylov A. M., Dohme F., Herr W., Stahl D. A., Gupta R. Secondary structure model for 23S ribosomal RNA. Nucleic Acids Res. 1981 Nov 25;9(22):6167–6189. doi: 10.1093/nar/9.22.6167. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Ojala D., Attardi G. Fine mapping of the ribosomal RNA genes of HeLa cell mitochondrial DNA. J Mol Biol. 1980 Apr;138(2):411–420. doi: 10.1016/0022-2836(80)90296-x. [DOI] [PubMed] [Google Scholar]
  24. Ranzini A. C., Dubin D. T. The "erythromycin-resistance" methylated sequence of Staphylococcus aureus ribosomal RNA. Plasmid. 1983 Nov;10(3):293–295. doi: 10.1016/0147-619x(83)90044-6. [DOI] [PubMed] [Google Scholar]
  25. Rubin C. M., Schmid C. W. Pyrimidine-specific chemical reactions useful for DNA sequencing. Nucleic Acids Res. 1980 Oct 24;8(20):4613–4619. doi: 10.1093/nar/8.20.4613. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Rüther U., Koenen M., Otto K., Müller-Hill B. pUR222, a vector for cloning and rapid chemical sequencing of DNA. Nucleic Acids Res. 1981 Aug 25;9(16):4087–4098. doi: 10.1093/nar/9.16.4087. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Saccone C., Cantatore P., Gadaleta G., Gallerani R., Lanave C., Pepe G., Kroon A. M. The nucleotide sequence of the large ribosomal RNA gene and the adjacent tRNA genes from rat mitochondria. Nucleic Acids Res. 1981 Aug 25;9(16):4139–4148. doi: 10.1093/nar/9.16.4139. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Sor F., Fukuhara H. Complete DNA sequence coding for the large ribosomal RNA of yeast mitochondria. Nucleic Acids Res. 1983 Jan 25;11(2):339–348. doi: 10.1093/nar/11.2.339. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Sor F., Fukuhara H. Identification of two erythromycin resistance mutations in the mitochondrial gene coding for the large ribosomal RNA in yeast. Nucleic Acids Res. 1982 Nov 11;10(21):6571–6577. doi: 10.1093/nar/10.21.6571. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Van Etten R. A., Bird J. W., Clayton D. A. Identification of the 3'-ends of the two mouse mitochondrial ribosomal RNAs. The 3'-end of 16 S ribosomal RNA contains nucleotides encoded by the gene for transfer RNALeuUUR. J Biol Chem. 1983 Aug 25;258(16):10104–10110. [PubMed] [Google Scholar]
  31. Veldman G. M., Klootwijk J., de Regt V. C., Planta R. J., Branlant C., Krol A., Ebel J. P. The primary and secondary structure of yeast 26S rRNA. Nucleic Acids Res. 1981 Dec 21;9(24):6935–6952. doi: 10.1093/nar/9.24.6935. [DOI] [PMC free article] [PubMed] [Google Scholar]
  32. de Bruijn M. H. Drosophila melanogaster mitochondrial DNA, a novel organization and genetic code. Nature. 1983 Jul 21;304(5923):234–241. doi: 10.1038/304234a0. [DOI] [PubMed] [Google Scholar]

Articles from Nucleic Acids Research are provided here courtesy of Oxford University Press

RESOURCES