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Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1981 Aug;78(8):4704–4707. doi: 10.1073/pnas.78.8.4704

Characterization and measurement of dehydroepiandrosterone sulfate in rat brain.

C Corpéchot, P Robel, M Axelson, J Sjövall, E E Baulieu
PMCID: PMC320231  PMID: 6458035

Abstract

Dehydroepiandrosterone (3 beta-hydroxy-5-androsten-17-one, I) sulfate (Ia) has been characterized in the anterior and the posterior parts of the brain of adult male rats. Its level (1.58 +/- 0.14 and 4.89 +/- 1.06 ng/g, mean +/- SD, in anterior and posterior brain, respectively) largely exceeded that of I in brain (0.42 +/- 0.10 and 0.12 +/- 0.03 ng/g in anterior and posterior brain, respectively) and of Ia in plasma (0.26 +/- 0.13 ng/ml). Brain Ia level did not seem to depend on adrenal secretion; it was unchanged after administration of corticotropin or dexamethasone for 3 days, and no meaningful change occurred in brain 15 days after adrenalectomy plus orchiectomy, compared with sham-operated controls. In contrast, stress conditions prevailing 2 days after adrenalectomy plus orchiectomy or after the corresponding sham operation resulted in a significantly increased concentration of Ia in the brain. Changes of Ia level in brain occurred irrespective of changes in corresponding plasma samples. It is proposed that Ia formation or accumulation (or both) in the rat brain depends on in situ mechanisms unrelated to the peripheral endocrine gland system.

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Selected References

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  1. Axelson M., Sjövall J. Analysis of unconjugated steroids in plasma by liquid-gel chromatography and glass capillary gas chromatography mass spectrometry. J Steroid Biochem. 1977 Jun;8(6):683–692. doi: 10.1016/0022-4731(77)90297-7. [DOI] [PubMed] [Google Scholar]
  2. Axelson M., Sjövall J. Selective liquid chromatographic isolation procedure for gas chromatographic-mass spectrometric analysis of 3-ketosteroids in biological materials. J Chromatogr. 1976 Nov 3;126:705–716. doi: 10.1016/s0021-9673(01)84113-3. [DOI] [PubMed] [Google Scholar]
  3. BAULIEU E. E., DRAY F. CONVERSION OF H3-DEHYDROISOANDROSTERONE (3BETA-HYDROXY-DELTA5-ANDROSTEN-17-ONE) SULFATE TO H3-ESTROGENS IN NORMAL PREGNANT WOMEN. J Clin Endocrinol Metab. 1963 Dec;23:1298–1301. doi: 10.1210/jcem-23-12-1298. [DOI] [PubMed] [Google Scholar]
  4. BAULIEU E. E. Studies of conjugated 17-ketosteroids in a case of adrenal tumor. J Clin Endocrinol Metab. 1962 May;22:501–510. doi: 10.1210/jcem-22-5-501. [DOI] [PubMed] [Google Scholar]
  5. BAULIEU E. E. [Ester-sulfates of steroid hormones. Isolation of the ester-sulfate of 5-androstene-3 beta-ol-17-one (dehydroepiandrosterone) in an adrenocortical tumor. Absence of the free steroid]. C R Seances Soc Biol Fil. 1960 Oct 3;251:1421–1423. [PubMed] [Google Scholar]
  6. BOLTE E., MANCUSO S., ERIKSSON G., WIQVIST N., DICZFALUSY E. STUDIES ON THE AROMATISATION OF NEUTRAL STEROIDS IN PREGNANT WOMEN. I. AROMATISATION OF C-19 STEROIDS BY PLACENTAS PERFUSED IN SITU. Acta Endocrinol (Copenh) 1964 Apr;45:535–559. doi: 10.1530/acta.0.0450535. [DOI] [PubMed] [Google Scholar]
  7. BURSTEIN S., LIEBERMAN S. Hydrolysis of ketosteroid hydrogen sulfates by solvolysis procedures. J Biol Chem. 1958 Aug;233(2):331–335. [PubMed] [Google Scholar]
  8. Brenner P. F., Guerrero R., Cekan Z., Diczfalusy E. Radioimmunoassay method for six steroids in human plasma. Steroids. 1973 Dec;22(6):775–794. doi: 10.1016/0039-128x(73)90053-6. [DOI] [PubMed] [Google Scholar]
  9. Corpéchot C., Robel P., Lachapelle F., Baumann N., Axelson M., Sjövall J., Baulieu E. E. Déhydroépiandrostérone libre et sulfo-conjuguée dans le cerveau de souris dysmyéliniques. C R Seances Acad Sci III. 1981 Jan 12;292(2):231–234. [PubMed] [Google Scholar]
  10. Cutler G. B., Jr, Glenn M., Bush M., Hodgen G. D., Graham C. E., Loriaux D. L. Adrenarche: a survey of rodents, domestic animals, and primates. Endocrinology. 1978 Dec;103(6):2112–2118. doi: 10.1210/endo-103-6-2112. [DOI] [PubMed] [Google Scholar]
  11. Denef C., Magnus C., McEwen B. S. Sex differences and hormonal control of testosterone metabolism in rat pituitary and brain. J Endocrinol. 1973 Dec;59(3):605–621. doi: 10.1677/joe.0.0590605. [DOI] [PubMed] [Google Scholar]
  12. Ellingboe J., Nyström E., Sjövall J. Liquid-gel chromatography on lipophilic-hydrophobic Sephadex derivatives. J Lipid Res. 1970 May;11(3):266–273. [PubMed] [Google Scholar]
  13. Grosser B. I., Bliss E. L. Metabolism of 11-hydroxysteroids by cerebral tissues in vitro. Steroids. 1966 Dec;8(6):915–928. doi: 10.1016/0039-128x(66)91018-x. [DOI] [PubMed] [Google Scholar]
  14. Guiraud J. M., Morfin R., Ducouret B., Samperez S., Jouan P. Pituitary metabolism of 5alpha-androstane-3beta-17beta-diol: intense and rapid conversion into 5alpha-androstane-3beta,6alpha,17beta-triol and 5alpha-androstane-3beta,7alpha, 17beta-triol. Steroids. 1979 Sep;34(3):241–248. doi: 10.1016/0039-128x(79)90076-x. [DOI] [PubMed] [Google Scholar]
  15. Hochberg R. B., Bandy L., Ponticorvo L., Lieberman S. Detection in bovine adrenal cortex of a lipoidal substance that yields pregnenolone upon treatment with alkali. Proc Natl Acad Sci U S A. 1977 Mar;74(3):941–945. doi: 10.1073/pnas.74.3.941. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Iwamori M., Moser H. W., Kishimoto Y. Cholesterol sulfate in rat tissues. Tissue distribution, developmental change and brain subcellular localization. Biochim Biophys Acta. 1976 Aug 23;441(2):268–279. doi: 10.1016/0005-2760(76)90170-3. [DOI] [PubMed] [Google Scholar]
  17. Iwamori M., Moser H. W., Kishimoto Y. Steroid sulfatase in brain: comparison of sulfohydrolase activities for various steroid sulfates in normal and pathological brains, including the various forms of metachromatic leukodystrophy. J Neurochem. 1976 Dec;27(6):1389–1395. doi: 10.1111/j.1471-4159.1976.tb02620.x. [DOI] [PubMed] [Google Scholar]
  18. Jaffe R. B. Testosterone metabolism in target tissues. Hypothalamic and pituitary tissues of the adult rat and human fetus, and the immature rat epiphysis. Steroids. 1969 Nov;14(5):483–498. doi: 10.1016/s0039-128x(69)80043-7. [DOI] [PubMed] [Google Scholar]
  19. Kishimoto Y., Sostek R. Activity of sterol-sulphate sulphohydrolase in rat brain: characterization, localization and change with age. J Neurochem. 1972 Jan;19(1):123–130. doi: 10.1111/j.1471-4159.1972.tb01261.x. [DOI] [PubMed] [Google Scholar]
  20. Knapstein P., David A., Wu C. H., Archer D. F., Flickinger G. L., Tochstone J. C. Metabolism of free and sulfoconjugated DHEA in brain tissue in vivo and in vitro. Steroids. 1968 Jun;11(6):885–896. doi: 10.1016/s0039-128x(68)80102-3. [DOI] [PubMed] [Google Scholar]
  21. LIPSETT M. B., COFFMAN G. D., NIXON W. E. LACK OF ACTIVITY OF DEHYDROEPIANDROSTERONE SULFATE IN METABOLIC BALANCE STUDIES. J Clin Endocrinol Metab. 1965 Jul;25:993–994. doi: 10.1210/jcem-25-7-993. [DOI] [PubMed] [Google Scholar]
  22. Linder M., Desfosses B., Emiliozzi R. Preparation of estrone and estradiol antigens through carbon 15 of these estrogens. Steroids. 1977 Feb;29(2):161–170. doi: 10.1016/0039-128x(77)90036-8. [DOI] [PubMed] [Google Scholar]
  23. Naftolin F., Ryan K. J., Petro Z. Aromatization of androstenedione by the anterior hypothalamus of adult male and female rats. Endocrinology. 1972 Jan;90(1):295–298. doi: 10.1210/endo-90-1-295. [DOI] [PubMed] [Google Scholar]
  24. Oftebro H., Størmer F. C., Pedersen J. L. The presence of an adrenodoxin-like ferredoxin and cytochrome P-450 in brain mitochondria. J Biol Chem. 1979 Jun 10;254(11):4331–4334. [PubMed] [Google Scholar]
  25. Robel P., Corpéchot C., Baulieu E. E. Testosterone and androstanolone in rat plasma and tissues. FEBS Lett. 1973 Jul 1;33(2):218–220. doi: 10.1016/0014-5793(73)80196-6. [DOI] [PubMed] [Google Scholar]
  26. Ruokonen A., Laatikainen T., Laitinen E. A., Vihko R. Free and sulfate-conjugated neutral steroids in human testis tissue. Biochemistry. 1972 Apr 11;11(8):1411–1416. doi: 10.1021/bi00758a013. [DOI] [PubMed] [Google Scholar]
  27. Sholiton L. J., Werk E. E. The less-polar metabolites produced by incubation of testosterone-4-14C with rat and bovine brain. Acta Endocrinol (Copenh) 1969 Aug;61(4):641–648. doi: 10.1530/acta.0.0610641. [DOI] [PubMed] [Google Scholar]
  28. VANDEWIELE R. L., MACDONALD P. C., GURPIDE E., LIEBERMAN S. STUDIES ON THE SECRETION AND INTERCONVERSION OF THE ANDROGENS. Recent Prog Horm Res. 1963;19:275–310. [PubMed] [Google Scholar]
  29. WIELAND R. G., LEVY R. P., KATZ D., HIRSCHMANN H. EVIDENCE FOR SECRETION OF 3 BETA-HYDROXYANDROST-5-EN-17-ONE SULFATE BY MEASUREMENT IN NORMAL HUMAN ADRENAL VENOUS BLOOD. Biochim Biophys Acta. 1963 Nov 15;78:566–568. doi: 10.1016/0006-3002(63)90930-2. [DOI] [PubMed] [Google Scholar]
  30. Winter J. S., Faiman C., Hobson W. C., Reyes F. I. The endocrine basis of sexual development in the chimpanzee. J Reprod Fertil Suppl. 1980;Suppl 28:131–138. [PubMed] [Google Scholar]

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