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Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1981 Aug;78(8):4843–4847. doi: 10.1073/pnas.78.8.4843

31P NMR studies of intact anchorage-dependent mouse embryo fibroblasts.

K Ugurbil, D L Guernsey, T R Brown, P Glynn, N Tobkes, I S Edelman
PMCID: PMC320269  PMID: 6946431

Abstract

31P NMR spectra of "normal" and x-ray-transformed anchorage-dependent mouse embryo fibroblasts (C3H/10T 1/2) at 145.7 MHz and 37 degrees C were obtained by using a solenoid coil probe with a sensitivity approximately 2.6 times higher than that of the standard Helmholtz coil probe. The cells were grown as monolayers on the surface of microcarrier beads. Results show that these cells have low levels of NMR-detectable ADP, are highly impermeable to protons, are capable of maintaining high ATP levels in the absence of respiration, and, when ATP generation is blocked, ultimately hydrolyze their existing ATP to a nonphosphorylated product and Pi. Only small differences were observed between the spectra of "normal" and transformed cells. However, the latter accumulated 2-deoxyglucose 6-phosphate at a faster rate.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BURTON K. A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid. Biochem J. 1956 Feb;62(2):315–323. doi: 10.1042/bj0620315. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Borek C., Miller R., Pain C., Troll W. Conditions for inhibiting and enhancing effects of the protease inhibitor antipain on x-ray-induced neoplastic transformation in hamster and mouse cells. Proc Natl Acad Sci U S A. 1979 Apr;76(4):1800–1803. doi: 10.1073/pnas.76.4.1800. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Cohen S. M., Ogawa S., Shulman R. G. 13C NMR studies of gluconeogenesis in rat liver cells: utilization of labeled glycerol by cells from euthyroid and hyperthyroid rats. Proc Natl Acad Sci U S A. 1979 Apr;76(4):1603–1609. doi: 10.1073/pnas.76.4.1603. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Daniel J. L., Molish I. R., Holmsen H. Radioactive labeling of the adenine nucleotide pool of cells as a method to distinguish among intracellular compartments. Studies on human platelets. Biochim Biophys Acta. 1980 Oct 15;632(3):444–453. doi: 10.1016/0304-4165(80)90240-8. [DOI] [PubMed] [Google Scholar]
  5. Holmsen H., Setkowsky C. A., Day H. J. Effects of antimycin and 2-deoxyglucose on adenine nucleotides in human platelets. Role of metabolic adenosine triphosphate in primary aggregation, secondary aggregation and shape change of platetets. Biochem J. 1974 Nov;144(2):385–396. doi: 10.1042/bj1440385. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  7. Moon R. B., Richards J. H. Determination of intracellular pH by 31P magnetic resonance. J Biol Chem. 1973 Oct 25;248(20):7276–7278. [PubMed] [Google Scholar]
  8. Navon G., Navon R., Shulman R. G., Yamane T. Phosphate metabolites in lymphoid, Friend erythroleukemia, and HeLa cells observed by high-resolution 31P nuclear magnetic resonance. Proc Natl Acad Sci U S A. 1978 Feb;75(2):891–895. doi: 10.1073/pnas.75.2.891. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Navon G., Ogawa S., Shulman R. G., Yamane T. 31P nuclear magnetic resonance studies of Ehrlich ascites tumor cells. Proc Natl Acad Sci U S A. 1977 Jan;74(1):87–91. doi: 10.1073/pnas.74.1.87. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Reznikoff C. A., Brankow D. W., Heidelberger C. Establishment and characterization of a cloned line of C3H mouse embryo cells sensitive to postconfluence inhibition of division. Cancer Res. 1973 Dec;33(12):3231–3238. [PubMed] [Google Scholar]
  11. Shulman R. G., Brown T. R., Ugurbil K., Ogawa S., Cohen S. M., den Hollander J. A. Cellular applications of 31P and 13C nuclear magnetic resonance. Science. 1979 Jul 13;205(4402):160–166. doi: 10.1126/science.36664. [DOI] [PubMed] [Google Scholar]
  12. Ugurbil K., Holmsen H., Shulman R. G. Adenine nucleotide storage and secretion in platelets as studied by 31P nuclear magnetic resonance. Proc Natl Acad Sci U S A. 1979 May;76(5):2227–2231. doi: 10.1073/pnas.76.5.2227. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Weber M. J., Hale A. H., Yau T. M., Buckman T., Johnson M., Brady T. M., LaRossa D. D. Transport changes associated with growth control and malignant transformation. J Cell Physiol. 1976 Dec;89(4):711–721. doi: 10.1002/jcp.1040890431. [DOI] [PubMed] [Google Scholar]

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