Skip to main content
PMC home page
Nucleic Acids Research logoLink to Nucleic Acids Research
. 1984 Nov 12;12(21):8115–8128. doi: 10.1093/nar/12.21.8115

Hormonal regulation of transcription of rDNA: use of nucleoside thiotriphosphates to measure initiation in isolated nuclei.

Z Y Zhang, E A Thompson, M R Stallcup
PMCID: PMC320285  PMID: 6095190

Abstract

Nuclei isolated from cultured mouse and rat cell lines initiated pre-rRNA chains at the correct site and with the correct nucleotide specificity (A for mouse, G for rat). Nucleic acid filter hybridization and S1 nuclease mapping were used to analyze the RNA products initiated with nucleoside (beta-S)triphosphates. Initiation of pre-rRNA was completely resistant to alpha-amanitin but was inhibited by either actinomycin D or heparin. Experiments with P1798 mouse lymphoma cells indicated that the antiproliferative effects of glucocorticoids on lymphoid cells includes a reduction in the ability of nuclei to initiate pre-rRNA chains.

Full text

PDF
8118

Images in this article

8121Image
on p.8121
8122Image
on p.8122
8122Image
on p.8122
8124Image
on p.8124
8125Image
on p.8125

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Arnheim N. Characterization of mouse ribosomal gene fragments purified by molecular cloning. Gene. 1979 Oct;7(2):83–96. doi: 10.1016/0378-1119(79)90025-8. [DOI] [PubMed] [Google Scholar]
  2. Cavanaugh A. H., Gokal P. K., Lawther R. P., Thompson E. A., Jr Glucocorticoid inhibition of initiation of transcription of the DNA encoding rRNA (rDNA) in lymphosarcoma P1798 cells. Proc Natl Acad Sci U S A. 1984 Feb;81(3):718–721. doi: 10.1073/pnas.81.3.718. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Cavannaugh A. H., Thompson E. A., Jr Hormonal regulation of transcription of rDNA. Inhibition of transcription during glucocorticoid-mediated inhibition of proliferation of lymphosarcoma P1798 cells in culture. J Biol Chem. 1983 Aug 25;258(16):9768–9773. [PubMed] [Google Scholar]
  4. Hipskind R. A., Reeder R. H. Initiation of ribosomal RNA chains in homogenates of oocyte nuclei. J Biol Chem. 1980 Aug 25;255(16):7896–7906. [PubMed] [Google Scholar]
  5. Maniatis T., Jeffrey A., van deSande H. Chain length determination of small double- and single-stranded DNA molecules by polyacrylamide gel electrophoresis. Biochemistry. 1975 Aug 26;14(17):3787–3794. doi: 10.1021/bi00688a010. [DOI] [PubMed] [Google Scholar]
  6. Miller K. G., Sollner-Webb B. Transcription of mouse rRNA genes by RNA polymerase I: in vitro and in vivo initiation and processing sites. Cell. 1981 Nov;27(1 Pt 2):165–174. doi: 10.1016/0092-8674(81)90370-6. [DOI] [PubMed] [Google Scholar]
  7. Prewitt R. S., Washington L. D., Stallcup M. R. Asymmetric transcription of mouse mammary tumor virus genes in vivo and in vitro. J Virol. 1984 Apr;50(1):60–65. doi: 10.1128/jvi.50.1.60-65.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Ringold G. M., Shank P. R., Varmus H. E., Ring J., Yamamoto K. R. Integration and transcription of mouse mammary tumor virus DNA in rat hepatoma cells. Proc Natl Acad Sci U S A. 1979 Feb;76(2):665–669. doi: 10.1073/pnas.76.2.665. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Ringold G., Lasfargues E. Y., Bishop J. M., Varmus H. E. Production of mouse mammary tumor virus by cultured cells in the absence and presence of hormones: assay by molecular hybridization. Virology. 1975 May;65(1):135–147. doi: 10.1016/0042-6822(75)90014-8. [DOI] [PubMed] [Google Scholar]
  10. Rothblum L. I., Reddy R., Cassidy B. Transcription initiation site of rat ribosomal DNA. Nucleic Acids Res. 1982 Nov 25;10(22):7345–7362. doi: 10.1093/nar/10.22.7345. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Smith M. M., Reeve A. E., Huang R. C. Analysis of RNA initiated in isolated mouse myeloma nuclei using purine nucleoside 5'[gamma-S]triphosphates as affinity probes. Cell. 1978 Oct;15(2):615–626. doi: 10.1016/0092-8674(78)90030-2. [DOI] [PubMed] [Google Scholar]
  12. Stallcup M. R., Washington L. D. Region-specific initiation of mouse mammary tumor virus RNA synthesis by endogenous RNA polymerase II in preparations of cell nuclei. J Biol Chem. 1983 Mar 10;258(5):2802–2807. [PubMed] [Google Scholar]
  13. Sun I. Y., Johnson E. M., Allfrey V. G. Initiation of transcription of ribosomal deoxyribonucleic acid sequences in isolated nuclei of Physarum polycephalum: studies using nucleoside 5'-[gamma-S]triphosphates and labeled precursors. Biochemistry. 1979 Oct 16;18(21):4572–4580. doi: 10.1021/bi00588a018. [DOI] [PubMed] [Google Scholar]
  14. Thompson E. A., Jr Properties of a cell-culture line derived from lymphosarcoma P1798. Mol Cell Endocrinol. 1980 Feb;17(2):95–102. doi: 10.1016/0303-7207(80)90121-5. [DOI] [PubMed] [Google Scholar]
  15. Wood K. M., Bowman L. H., Thompson E. A., Jr Transcription of the mouse ribosomal spacer region. Mol Cell Biol. 1984 May;4(5):822–828. doi: 10.1128/mcb.4.5.822. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Nucleic Acids Research are provided here courtesy of Oxford University Press

RESOURCES