Skip to main content
PMC home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1981 Aug;78(8):5221–5224. doi: 10.1073/pnas.78.8.5221

Identification, characterization, and distribution of secretin immunoreactivity in rat and pig brain.

T L O'Donohue, C G Charlton, R L Miller, G Boden, D M Jacobowitz
PMCID: PMC320380  PMID: 6946469

Abstract

Secretin immunoreactivity was detected in the central nervous system of the rat and pig with a highly specific radioimmunoassay. The secretin immunoreactivity in the rat and pig brain and duodenum extracts was fractionated by using a reverse-phase high-pressure liquid chromatographic system. The immunoreactive secretin from pig brain and duodenum coeluted precisely with synthetic porcine secretin. However, immunoreactive secretin extracted from rat brain and duodenum eluted slightly before porcine secretin. These data suggest a slight difference in the structure of rat and pig secretin. The detection of secretin in the brain lays the groundwork for studies to determine the role of the peptide in central nervous system function.

Full text

PDF
5223

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bayliss W. M., Starling E. H. The mechanism of pancreatic secretion. J Physiol. 1902 Sep 12;28(5):325–353. doi: 10.1113/jphysiol.1902.sp000920. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Boden G., Essa N., Owen O. E. Effects of intraduodenal amino acids, fatty acids, and sugars on secretin concentrations. Gastroenterology. 1975 Apr;68(4 Pt 1):722–727. [PubMed] [Google Scholar]
  3. Boden G., Essa N., Owen O. E., Reichle F. A. Effects of intraduodenal administration of HCl and glucose on circulating immunoreactive secretin and insulin concentrations. J Clin Invest. 1974 Apr;53(4):1185–1193. doi: 10.1172/JCI107657. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Bryant M. G., Polak M. M., Modlin I., Bloom S. R., Albuquerque R. H., Pearse A. G. Possible dual role for vasoactive intestinal peptide as gastrointestinal hormone and neurotransmitter substance. Lancet. 1976 May 8;1(7967):991–993. doi: 10.1016/s0140-6736(76)91863-8. [DOI] [PubMed] [Google Scholar]
  5. Burhol P. G., Waldum H. L. Radioimmunoassay of secretin in acidified plasma. Acta Hepatogastroenterol (Stuttg) 1978 Dec;25(6):474–481. [PubMed] [Google Scholar]
  6. Chey W. Y., Kim M. S., Lee K. Y., Chang T. M. Effect of rabbit antisecretin serum on postprandial pancreatic secretion in dogs. Gastroenterology. 1979 Dec;77(6):1268–1275. [PubMed] [Google Scholar]
  7. Conlon J. M., Samson W. K., Dobbs R. E., Orci L., Unger R. H. Glucagon-like polypeptides in canine brain. Diabetes. 1979 Jul;28(7):700–702. doi: 10.2337/diab.28.7.700. [DOI] [PubMed] [Google Scholar]
  8. Fuxe K., Andersson K., Hökfelt T., Mutt V., Ferland L., Agnati L. F., Ganten D., Said S., Eneroth P., Gustafsson J. A. Localization and possible function of peptidergic neurons and their interactions with central catecholamine neurons, and the central actions of gut hormones. Fed Proc. 1979 Aug;38(9):2333–2340. [PubMed] [Google Scholar]
  9. GREENWOOD F. C., HUNTER W. M., GLOVER J. S. THE PREPARATION OF I-131-LABELLED HUMAN GROWTH HORMONE OF HIGH SPECIFIC RADIOACTIVITY. Biochem J. 1963 Oct;89:114–123. doi: 10.1042/bj0890114. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Jorpes J. E., Mutt V. On the biological assay of secretin. The reference standard. Acta Physiol Scand. 1966 Mar;66(3):316–325. doi: 10.1111/j.1748-1716.1966.tb03205.x. [DOI] [PubMed] [Google Scholar]
  11. Larsson L. I., Fahrenkrug J., Schaffalitzky De Muckadell O., Sundler F., Håkanson R., Rehfeld J. R. Localization of vasoactive intestinal polypeptide (VIP) to central and peripheral neurons. Proc Natl Acad Sci U S A. 1976 Sep;73(9):3197–3200. doi: 10.1073/pnas.73.9.3197. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Lorén I., Alumets J., Håkanson R., Sundler F., Thorell J. Gut-type glucagon immunoreactivity in nerves of the rat brain. Histochemistry. 1979 Jul 11;61(3):335–341. doi: 10.1007/BF00508455. [DOI] [PubMed] [Google Scholar]
  13. Mutt V., Carlquist M., Tatemoto K. Secretin-like bioactivity in extracts of porcine brain. Life Sci. 1979 Nov 12;25(20):1703–1707. doi: 10.1016/0024-3205(79)90472-7. [DOI] [PubMed] [Google Scholar]
  14. Propst F., Moroder L., Wünsch E., Hamprecht B. The influence of secretin, glucagon and other peptides, of amino acids, prostaglandin endoperoxide analogues and diazepam on the level of adenosine 3',5'-cyclic monophosphate in neuroblastoma x glioma hybrid cells. J Neurochem. 1979 May;32(5):1495–1500. doi: 10.1111/j.1471-4159.1979.tb11090.x. [DOI] [PubMed] [Google Scholar]
  15. Said S. I., Rosenberg R. N. Vasoactive intestinal polypeptide: abundant immunoreactivity in neural cell lines and normal nervous tissue. Science. 1976 May 28;192(4242):907–908. doi: 10.1126/science.1273576. [DOI] [PubMed] [Google Scholar]
  16. Tager H., Hohenboken M., Markese J., Dinerstein R. J. Identification and localization of glucagon-related peptides in rat brain. Proc Natl Acad Sci U S A. 1980 Oct;77(10):6229–6233. doi: 10.1073/pnas.77.10.6229. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. van Calker D., Müller M., Hamprecht B. Regulation by secretin, vasoactive intestinal peptide, and somatostatin of cyclic AMP accumulation in cultured brain cells. Proc Natl Acad Sci U S A. 1980 Nov;77(11):6907–6911. doi: 10.1073/pnas.77.11.6907. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES