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. 1984 Dec 21;12(24):9473–9487. doi: 10.1093/nar/12.24.9473

Complete nucleotide sequence of the murine H-2Kk gene. Comparison of three H-2K locus alleles.

B Arnold, H G Burgert, A L Archibald, S Kvist
PMCID: PMC320474  PMID: 6096831

Abstract

We have determined the DNA sequence of the H-2Kk gene of the mouse major histocompatibility complex (MHC). Comparison on the nucleotide and protein level of three H-2K alleles (Kk, Kb and Kd) reveals a high degree of homology, in particular between the Kb and Kk alleles. Differences between the two latter antigens are almost exclusively confined to the alpha 1 and alpha 2 domains. At nine positions in the extracellular part of the molecules we have found allele-specific amino acids. Interestingly, 78% of these residues are either polar or carry hydroxyl-groups. This makes it likely that they are exposed on the surface of the molecules and might then be part of antigenic determinants. We have also identified potentially allele-specific nucleotide sequences of the K genes which might be used as specific DNA probes.

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Selected References

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