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. 1982 Mar 25;10(6):1867–1876. doi: 10.1093/nar/10.6.1867

A comparison of experimental and theoretical melting maps for replicative form of phi X174 DNA.

Lyubchenko YuL, Kalambet YuA, V I Lyamichev, A S Borovik
PMCID: PMC320577  PMID: 6210880

Abstract

A previously elaborated technique for fixing a chosen partially melted state of DNA with glyoxal was used in a study of the melting process of the replicative form (RF III) of phi X174 DNA. Electron-microscopic maps corresponding to five points of the melting curve of RF III were obtained and compared with the theoretical melting maps obtained in (4) and (6). This comparison clearly shows that only rigorous calculations (4) and not the ones proposed by Azbel (6,7) correctly predict the course of RF III melting.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Azbel M. Y. DNA sequencing and helix-coil transition. III. DNA sequencing. Biopolymers. 1980 Jan;19(1):95–109. doi: 10.1002/bip.1980.360190107. [DOI] [PubMed] [Google Scholar]
  2. Azbel M. Y. DNA sequencing and melting curve. Proc Natl Acad Sci U S A. 1979 Jan;76(1):101–105. doi: 10.1073/pnas.76.1.101. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Borovik A. S., Kalambet Y. A., Lyubchenko Y. L., Shitov V. T., Golovanov E. I. Equilibrium melting of plasmid ColE1 DNA: electron-microscopic visualization. Nucleic Acids Res. 1980 Sep 25;8(18):4165–4184. doi: 10.1093/nar/8.18.4165. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Fixman M., Freire J. J. Theory of DNA melting curves. Biopolymers. 1977 Dec;16(12):2693–2704. doi: 10.1002/bip.1977.360161209. [DOI] [PubMed] [Google Scholar]
  5. Gabbarro-Arpa J., Tougard P., Reiss C. Correlation of local stability of DNA during melting with environmental conditions. Nature. 1979 Aug 9;280(5722):515–517. doi: 10.1038/280515a0. [DOI] [PubMed] [Google Scholar]
  6. Lyubchenko Y. L., Vologodskii A. V., Frank-Kamenetskii M. D. Direct comparison of theoretical and experimental melting profiles for RF II phiX174 DNA. Nature. 1978 Jan 5;271(5640):28–31. doi: 10.1038/271028a0. [DOI] [PubMed] [Google Scholar]
  7. Sanger F., Air G. M., Barrell B. G., Brown N. L., Coulson A. R., Fiddes C. A., Hutchison C. A., Slocombe P. M., Smith M. Nucleotide sequence of bacteriophage phi X174 DNA. Nature. 1977 Feb 24;265(5596):687–695. doi: 10.1038/265687a0. [DOI] [PubMed] [Google Scholar]
  8. Vologodskii A. V., Frank-Kamenetskii M. D. Theoretical melting profiles and denaturation maps of DNA with known sequence: fdDNA. Nucleic Acids Res. 1978 Jul;5(7):2547–2556. doi: 10.1093/nar/5.7.2547. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Wada A., Yabuki S., Husimi Y. Fine structure in the thermal denaturation of DNA: high temperature-resolution spectrophotometric studies. CRC Crit Rev Biochem. 1980;9(2):87–144. doi: 10.3109/10409238009105432. [DOI] [PubMed] [Google Scholar]

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