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. 1982 Jun 11;10(11):3561–3571. doi: 10.1093/nar/10.11.3561

Length changes in solution accompanying the B-Z transition of poly (dG-m5dC) induced by Co(NH3)63+.

H H Chen, E Charney, D C Rau
PMCID: PMC320731  PMID: 7099967

Abstract

Transient electric dichroism measurements have been used to observe the rotational relaxation times of 145 base pair fragments of poly (dGm5dC) and random sequence DNA to solution. From these the lengths of the fragments are calculated and the interbase pair separation or rise per base pair (RPB) calculated. The observations show that even in low salt, the addition of very low concentrations of trivalent Co(NH3)63+ results in a transition of the dGm5dC polymer from B-form to Z-form with a change in the RPB from 3.4 +/- .06A to 3.7 +/- .06A, the latter form defined by the criterion of an inverted circular dichroism spectra similar to that observed at high salt in the absence of Co(NH3)63+. The 145 base pair DNA and poly (dGm5dC) are found to be essentially fully extended rods in low salt (0.2 - 2 mM Na+) solutions.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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