Skip to main content
PMC home page
Nucleic Acids Research logoLink to Nucleic Acids Research
. 1982 Jul 10;10(13):4059–4069. doi: 10.1093/nar/10.13.4059

Synthesis and physicochemical properties of two analogs of poly(dA): poly(2-aminopurine-9-beta-D-deoxyribonucleotide) and poly 2-amino-deoxyadenylic acid.

K H Scheit, H R Rackwitz
PMCID: PMC320778  PMID: 6287431

Abstract

Polymerization of chemically synthesized dn2h6ATP and dn2ATP by deoxynucleotidyl transferase from calf thymus furnished poly(dn2h6A) and poly(dn2A) respectively. The synthetic polynucleotides were characterized by spectroscopic, ultracentrifugation and enzymatic methods. In polynucleotide-polynucleotide interaction, poly(dn2h6A) and poly(dn2A) behaved like analogs of poly(dnA).

Full text

PDF
4068

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bessman M. J., Muzyczka N., Goodman M. F., Schnaar R. L. Studies on the biochemical basis of spontaneous mutation. II. The incorporation of a base and its analogue into DNA by wild-type, mutator and antimutator DNA polymerases. J Mol Biol. 1974 Sep 15;88(2):409–421. doi: 10.1016/0022-2836(74)90491-4. [DOI] [PubMed] [Google Scholar]
  2. Bähr W., Faerber P., Scheit K. H. The effects of thioketo substitution upon uracil-adenine interactions in polyribonucleotides. Synthesis and properties of poly (2-thiouridylic acid) and poly(2,4-dithiouridylic acid). Eur J Biochem. 1973 Mar 15;33(3):535–544. doi: 10.1111/j.1432-1033.1973.tb02713.x. [DOI] [PubMed] [Google Scholar]
  3. Cerami A., Reich E., Ward D. C., Goldberg I. H. The interaction of actinomycin with DNA: requirement for the 2-amino group of purines. Proc Natl Acad Sci U S A. 1967 Apr;57(4):1036–1042. doi: 10.1073/pnas.57.4.1036. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Chamberlin M. J. Comparative properties of DNA, RNA, and hybrid homopolymer pairs. Fed Proc. 1965 Nov-Dec;24(6):1446–1457. [PubMed] [Google Scholar]
  5. Howard F. B., Frazier J., Miles H. T. A new polynucleotide complex stabilized by 3 interbase hydrogen bonds, poly-2-aminoadenylic acid + polyuridylic acid. J Biol Chem. 1966 Sep 25;241(18):4293–4295. [PubMed] [Google Scholar]
  6. Janion C., Scheit K. H. The effect of thioketo substitution on uracil-2-aminopurine and uracil-2, 6-diaminopurine interactions in polynucleotides. Biochim Biophys Acta. 1976 May 3;432(2):192–198. doi: 10.1016/0005-2787(76)90161-1. [DOI] [PubMed] [Google Scholar]
  7. Janion C., Shugar D. Preparation and properties of poly 2-aminopurine ribotidylic acid. Acta Biochim Pol. 1973;20(3):271–284. [PubMed] [Google Scholar]
  8. Koch R. E. The influence of neighboring base pairs upon base-pair substitution mutation rates. Proc Natl Acad Sci U S A. 1971 Apr;68(4):773–776. doi: 10.1073/pnas.68.4.773. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. McClure W. R., Scheit K. H. Enzyme kinetic parameters of the fluorescent ATP analogue, 2-aminopurine triphosphate. FEBS Lett. 1973 Jun 1;32(2):267–269. doi: 10.1016/0014-5793(73)80849-x. [DOI] [PubMed] [Google Scholar]
  10. Rackwitz H. R., Scheit K. H. The stereochemical basis of template function. Eur J Biochem. 1977 Jan 3;72(1):191–200. doi: 10.1111/j.1432-1033.1977.tb11239.x. [DOI] [PubMed] [Google Scholar]
  11. Rogan E. G., Bessman M. J. Studies on the pathway of incorporation of 2-aminopurine into the deoxyribonucleic acid of Escherichia coli. J Bacteriol. 1970 Sep;103(3):622–633. doi: 10.1128/jb.103.3.622-633.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Ward D. C., Reich E., Stryer L. Fluorescence studies of nucleotides and polynucleotides. I. Formycin, 2-aminopurine riboside, 2,6-diaminopurine riboside, and their derivatives. J Biol Chem. 1969 Mar 10;244(5):1228–1237. [PubMed] [Google Scholar]

Articles from Nucleic Acids Research are provided here courtesy of Oxford University Press

RESOURCES