Abstract
The nucleotide sequence of the region between the genes for the large subunit of ribulose-1, 5-bisphosphate carboxylase/oxygenase and the beta subunit of coupling factor from tobacco chloroplast DNA has been determined. The 5' ends of their mRNAs have been located on the DNA sequence using s1 nuclease mapping. The promoter sequences of these chloroplast genes have features in common with those of prokaryotes. E. coli RNA polymerase can recognize these sequences and start transcription at authentic initiation sites.
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- Bedbrook J. R., Link G., Coen D. M., Bogorad L. Maize plastid gene expressed during photoregulated development. Proc Natl Acad Sci U S A. 1978 Jul;75(7):3060–3064. doi: 10.1073/pnas.75.7.3060. [DOI] [PMC free article] [PubMed] [Google Scholar]
- De Heij J. T., Groot G. S. Spirodela oligorhiza chloroplast DNA codes for ATPase subunits alpha and beta. Immunological evidence from a coupled transcription-translation system. FEBS Lett. 1981 Nov 2;134(1):6–10. doi: 10.1016/0014-5793(81)80538-8. [DOI] [PubMed] [Google Scholar]
- Doherty A., Gray J. C. Synthesis of a dicyclohexylcarbodiimide-binding proteolipid by isolated pea chloroplasts. Eur J Biochem. 1980;108(1):131–136. doi: 10.1111/j.1432-1033.1980.tb04703.x. [DOI] [PubMed] [Google Scholar]
- Driesel A. J., Speirs J., Bohnert H. J. Spinach chloroplast mRNA for a 32 000 dalton polypeptide: size and localization on the physical map of the chloroplast DNA. Biochim Biophys Acta. 1980 Dec 11;610(2):297–310. doi: 10.1016/0005-2787(80)90011-8. [DOI] [PubMed] [Google Scholar]
- Gatenby A. A., Castleton J. A., Saul M. W. Expression in E. coli of maize and wheat chloroplast genes for large subunit of ribulose bisphosphate carboxylase. Nature. 1981 May 14;291(5811):117–121. doi: 10.1038/291117a0. [DOI] [PubMed] [Google Scholar]
- Gilbert S. F., de Boer H. A., Nomura M. Identification of initiation sites for the in vitro transcription of rRNA operons rrnE and rrnA in E. coli. Cell. 1979 May;17(1):211–224. doi: 10.1016/0092-8674(79)90309-x. [DOI] [PubMed] [Google Scholar]
- Hartley S. E., McBeath S. Cytogenetic follow-up in chronic myeloid leukemia. Cancer Genet Cytogenet. 1981 Jan;3(1):37–46. doi: 10.1016/0165-4608(81)90054-6. [DOI] [PubMed] [Google Scholar]
- Herrmann R. G., Possingham J. V. Plastid DNA-the plastome. Results Probl Cell Differ. 1980;10:45–96. doi: 10.1007/978-3-540-38255-3_3. [DOI] [PubMed] [Google Scholar]
- Jolly S. O., McIntosh L., Link G., Bogorad L. Differential transcription in vivo and in vitro of two adjacent maize chloroplast genes: The large subunit of ribulosebisphosphate carboxylase and the 2.2-kilobase gene. Proc Natl Acad Sci U S A. 1981 Nov;78(11):6821–6825. doi: 10.1073/pnas.78.11.6821. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kato A., Shimada H., Kusuda M., Sugiura M. The nucleotide sequences of two tRNAAsn genes from tobacco chloroplasts. Nucleic Acids Res. 1981 Nov 11;9(21):5601–5607. doi: 10.1093/nar/9.21.5601. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Link G., Bogorad L. Sizes, locations, and directions of transcription of two genes on a cloned maize chloroplast DNA sequence. Proc Natl Acad Sci U S A. 1980 Apr;77(4):1832–1836. doi: 10.1073/pnas.77.4.1832. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Maxam A. M., Gilbert W. A new method for sequencing DNA. Proc Natl Acad Sci U S A. 1977 Feb;74(2):560–564. doi: 10.1073/pnas.74.2.560. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Mendiola-Morgenthaler L. R., Morgenthaler J. J., Price C. A. Synthesis of coupling factor CF1 protein by isolated spinach chloroplasts. FEBS Lett. 1976 Feb 1;62(1):96–100. doi: 10.1016/0014-5793(76)80025-7. [DOI] [PubMed] [Google Scholar]
- Nelson N., Nelson H., Schatz G. Biosynthesis and assembly of the proton-translocating adenosine triphosphatase complex from chloroplasts. Proc Natl Acad Sci U S A. 1980 Mar;77(3):1361–1364. doi: 10.1073/pnas.77.3.1361. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Pribnow D. Nucleotide sequence of an RNA polymerase binding site at an early T7 promoter. Proc Natl Acad Sci U S A. 1975 Mar;72(3):784–788. doi: 10.1073/pnas.72.3.784. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Schaller H., Gray C., Herrmann K. Nucleotide sequence of an RNA polymerase binding site from the DNA of bacteriophage fd. Proc Natl Acad Sci U S A. 1975 Feb;72(2):737–741. doi: 10.1073/pnas.72.2.737. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sugiura M., Miura K. Transcription of double-stranded RNA by Escherichia coli DNA-dependent RNA polymerase. Eur J Biochem. 1977 Feb 15;73(1):179–184. doi: 10.1111/j.1432-1033.1977.tb11305.x. [DOI] [PubMed] [Google Scholar]
- Tohdoh N., Sugiura M. The complete nucleotide sequence of 16S ribosomal RNA gene from tobacco chloroplasts. Gene. 1982 Feb;17(2):213–218. doi: 10.1016/0378-1119(82)90074-9. [DOI] [PubMed] [Google Scholar]
- Urano Y., Kominami R., Mishima Y., Muramatsu M. The nucleotide sequence of the putative transcription initiation site of a cloned ribosomal RNA gene of the mouse. Nucleic Acids Res. 1980 Dec 20;8(24):6043–6058. doi: 10.1093/nar/8.24.6043. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Wollgiehn R., Parthier B. RNA and protein synthesis in plastid differentiation. Results Probl Cell Differ. 1980;10:97–145. doi: 10.1007/978-3-540-38255-3_4. [DOI] [PubMed] [Google Scholar]
- Zurawski G., Perrot B., Bottomley W., Whitfeld P. R. The structure of the gene for the large subunit of ribulose 1,5-bisphosphate carboxylase from spinach chloroplast DNA. Nucleic Acids Res. 1981 Jul 24;9(14):3251–3270. doi: 10.1093/nar/9.14.3251. [DOI] [PMC free article] [PubMed] [Google Scholar]