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. 1982 Sep 25;10(18):5605–5620. doi: 10.1093/nar/10.18.5605

Improvements to solid phase phosphotriester synthesis of deoxyoligonucleotides.

T P Patel, T A Millican, C C Bose, R C Titmas, G A Mock, M A Eaton
PMCID: PMC320910  PMID: 7145710

Abstract

A solution of benzenesulphonic acid (3%, w/v) in a dimethylformamide and dichloromethane mixture (9:1, v/v) is shown to be a very effective reagent for the detritylation of deoxyoligonucleotides attached to a solid support. The levels of depurination with this reagent were lower than those observed with other reagents such as trichloroacetic acid. Coupling reactions are described using above ambient temperatures with no detectable increase in side products. Both procedures have been successfully incorporated into an automated system, which can compete with the rate of synthesis by the phosphite approach.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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