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. 1982 Sep 25;10(18):5649–5662. doi: 10.1093/nar/10.18.5649

cis 2-Methylthio-ribosylzeatin (ms2io6A) is present in the transfer RNA of Salmonella typhimurium, but not Escherichia coli.

M Buck, J A McCloskey, B Basile, B N Ames
PMCID: PMC320913  PMID: 6755395

Abstract

We have identified the cis isomer of N6-(4-hydroxy-isopentenyl)-2-methylthioadenosine (ms2io6A) as a component of the tRNA of Salmonella typhimurium. This is the first report of this compound in the tRNA of any member of the enterobacteriaceae: the nucleoside was previously thought to be found exclusively in plants or plant associated bacteria. Interestingly, all E. coli strains examined were found to lack ms2io6A. Evidence is presented which suggests S. typhimurium tRNA also contains low levels of 5-carboxymethylaminomethyl-2-thiouridine (cmnm5s2U) in addition to 5-methylaminomethyl-2-thiouridine (mnm5s2U).

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Selected References

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  1. Alam S. N., Hall R. H. Separation of the delta-2 and delta-3 isomers of N6-(delta-2-isopentenyl)adenosine by gas chromatography and characterization by mass spectroscopy. Anal Biochem. 1971 Apr;40(2):424–428. doi: 10.1016/0003-2697(71)90402-7. [DOI] [PubMed] [Google Scholar]
  2. Alper M. D., Ames B. N. Transport of antibiotics and metabolite analogs by systems under cyclic AMP control: positive selection of Salmonella typhimurium cya and crp mutants. J Bacteriol. 1978 Jan;133(1):149–157. doi: 10.1128/jb.133.1.149-157.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Buck M., Griffiths E. Iron mediated methylthiolation of tRNA as a regulator of operon expression in Escherichia coli. Nucleic Acids Res. 1982 Apr 24;10(8):2609–2624. doi: 10.1093/nar/10.8.2609. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Cimino F., Traboni C., Colonna A., Izzo P., Salvatore F. Purification and properties of several transfer RNA methyltransferases from S. typhimurium. Mol Cell Biochem. 1981 Apr 27;36(2):95–104. doi: 10.1007/BF02354908. [DOI] [PubMed] [Google Scholar]
  5. Eisenberg S. P., Yarus M., Soll L. The effect of an Escherichia coli regulatory mutation on transfer RNA structure. J Mol Biol. 1979 Nov 25;135(1):111–126. doi: 10.1016/0022-2836(79)90343-7. [DOI] [PubMed] [Google Scholar]
  6. Gefter M. L., Russell R. L. Role modifications in tyrosine transfer RNA: a modified base affecting ribosome binding. J Mol Biol. 1969 Jan 14;39(1):145–157. doi: 10.1016/0022-2836(69)90339-8. [DOI] [PubMed] [Google Scholar]
  7. Green C. J., Kammen H. O., Penhoet E. E. Purification and properties of a mammalian tRNA pseudouridine synthase. J Biol Chem. 1982 Mar 25;257(6):3045–3052. [PubMed] [Google Scholar]
  8. Hashizume T., Sugiyama T., Imura M., Cory H. T., Scott M. F., McCloskey J. A. Determination of cytokinins by mass spectrometry based on stable isotope dilution. Anal Biochem. 1979 Jan 1;92(1):111–122. doi: 10.1016/0003-2697(79)90631-6. [DOI] [PubMed] [Google Scholar]
  9. Hoburg A., Aschhoff H. J., Kersten H., Manderschied U., Gassen H. G. Function of modified nucleosides 7-methylguanosine, ribothymidine, and 2-thiomethyl-N6-(isopentenyl)adenosine in procaryotic transfer ribonucleic acid. J Bacteriol. 1979 Nov;140(2):408–414. doi: 10.1128/jb.140.2.408-414.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. McLennan B. D., Buck M., Humphreys J., Griffiths E. Iron-related modification of bacterial transfer RNA. Nucleic Acids Res. 1981 Jun 11;9(11):2629–2640. doi: 10.1093/nar/9.11.2629. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Menichi B., Heyman T. Study of tyrosine transfer ribonucleic acid modification in relation to sporulation in Bacillus subtilis. J Bacteriol. 1976 Jul;127(1):268–280. doi: 10.1128/jb.127.1.268-280.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Morris R. O., Regier D. A., Olson R. M., Jr, Struxness L. A., Armstrong D. J. Distribution of cytokinin-active nucleosides in isoaccepting transfer ribonucleic acids from Agrobacterium tumefaciens. Biochemistry. 1981 Oct 13;20(21):6012–6017. doi: 10.1021/bi00524a014. [DOI] [PubMed] [Google Scholar]
  13. Munns T. W., Liszewski M. K., Sims H. F. Characterization of antibodies specific for N6-methyladenosine and for 7-methylguanosine. Biochemistry. 1977 May 17;16(10):2163–2168. doi: 10.1021/bi00629a019. [DOI] [PubMed] [Google Scholar]
  14. Playtis A. J., Leonard N. J. The synthesis of ribosyl-cis-zeatin and thin layer chromatographic separation of the cis and trans isomers of ribosylzeatin. Biochem Biophys Res Commun. 1971 Oct 1;45(1):1–5. doi: 10.1016/0006-291x(71)90041-6. [DOI] [PubMed] [Google Scholar]
  15. Regier D. A., Morris R. O. Secretion of trans-zeatin by Agrobacterium tumefaciens: a function determined by the nopaline Ti plasmid. Biochem Biophys Res Commun. 1982 Feb 26;104(4):1560–1566. doi: 10.1016/0006-291x(82)91429-2. [DOI] [PubMed] [Google Scholar]
  16. Sanderson K. E. Genetic relatedness in the family Enterobacteriaceae. Annu Rev Microbiol. 1976;30:327–349. doi: 10.1146/annurev.mi.30.100176.001551. [DOI] [PubMed] [Google Scholar]
  17. Smith H. R., Humphreys G. O., Grindley N. D., Grindley J. N., Anderson E. S. Molecular studies of an fi+ plasmid from strains of Salmonella typhimurium. Mol Gen Genet. 1973 Nov 2;126(2):143–151. doi: 10.1007/BF00330989. [DOI] [PubMed] [Google Scholar]
  18. Thimmappaya B., Cherayil J. D. Unique presence of 2-methylthio-ribosylzeatin in the transfer ribonucleic acid of the bacterium Pseudomonas aeruginosa. Biochem Biophys Res Commun. 1974 Sep 23;60(2):665–672. doi: 10.1016/0006-291x(74)90292-7. [DOI] [PubMed] [Google Scholar]
  19. Vold B. S., Keith D. E., Jr, Buck M., McCloskey J. A., Pang H. Lysine tRNAs from Bacillus subtilis 168: structural analysis. Nucleic Acids Res. 1982 May 25;10(10):3125–3132. doi: 10.1093/nar/10.10.3125. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Vold B. S. Production and characterization of antibodies and establishment of a radioimmunoassay for ribosylzeatin. Plant Physiol. 1981 Mar;67(3):401–403. doi: 10.1104/pp.67.3.401. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Yanofsky C. Mutations affecting tRNATrp and its charging and their effect on regulation of transcription termination at the attenuator of the tryptophan operon. J Mol Biol. 1977 Jul 15;113(4):663–677. doi: 10.1016/0022-2836(77)90229-7. [DOI] [PubMed] [Google Scholar]
  22. Zurawski G., Brown K., Killingly D., Yanofsky C. Nucleotide sequence of the leader region of the phenylalanine operon of Escherichia coli. Proc Natl Acad Sci U S A. 1978 Sep;75(9):4271–4275. doi: 10.1073/pnas.75.9.4271. [DOI] [PMC free article] [PubMed] [Google Scholar]

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