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. 1985 Jul 25;13(14):5019–5026. doi: 10.1093/nar/13.14.5019

Altered DNA contacts made by a mutant AraC protein.

A Brunelle, W Hendrickson, R Schleif
PMCID: PMC321846  PMID: 3895160

Abstract

Mutant AraC proteins were selected for their ability to induce but not to repress, or their ability to repress but not to induce the araBAD operon. One such unusual mutant is able to bind to the araI site with an affinity only two to three-fold weaker than the wild type AraC protein, but the mutant protein was shown, both in crude extracts and when purified, to contact only two of the three major groove regions of the DNA that are contacted by the wild type protein.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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