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. 1985 Jul 25;13(14):5157–5172. doi: 10.1093/nar/13.14.5157

Changes of chromatin conformation around mouse interferon-beta gene associated with induction of interferon synthesis.

Y Higashi
PMCID: PMC321856  PMID: 2410861

Abstract

Changes of chromatin structure around the mouse interferon-beta gene associated with induction were examined by the use of sensitivity to digestion with DNaseI. Upon induction, the DNaseI-sensitivity of mouse interferon-beta gene was increased and seven DNaseI-hypersensitive sites were mapped. One of them, which was located at approximately 100bp upstream and was insensitive in the uninduced state, showed a marked sensitivity upon induction. Four others, located at 580bp and 430bp upstream, and 1.9kb and 2.2kb downstream, whose levels of DNaseI-sensitivity were very low in the uninduced state, showed significant sensitivity in the induced state. Two remaining sites, located at 7.5kb upstream and 7.6kb downstream, had relatively high DNaseI-sensitivities even in the uninduced state and slightly increased their sensitivities upon induction.

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