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. 1985 Nov 25;13(22):8119–8128. doi: 10.1093/nar/13.22.8119

Nucleotide sequence encoding the biosynthetic dehydroquinase function of the penta-functional arom locus of Aspergillus nidulans.

I G Charles, J W Keyte, W J Brammar, A R Hawkins
PMCID: PMC322114  PMID: 3906567

Abstract

The nucleotide sequence of a 1.9 Kb HindIII fragment of DNA derived from the arom locus of A.nidulans and encoding the biosynthetic dehydroquinase activity has been determined. The sequences encoding the biosynthetic and catabolic dehydroquinase enzymes of A.nidulans show no detectable homology, strongly suggesting convergent evolutionary pathways. The messenger RNA specified by the arom locus was detected as a 5.3 Kb RNA species.

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Selected References

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  1. Ahmed S. I., Giles N. H. Organization of enzymes in the common aromatic synthetic pathway: evidence for aggregation in fungi. J Bacteriol. 1969 Jul;99(1):231–237. doi: 10.1128/jb.99.1.231-237.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Armitt S., McCullough W., Roberts C. F. Analysis of acetate non-utilizing (acu) mutants in Aspergillus nidulans. J Gen Microbiol. 1976 Feb;92(2):263–282. doi: 10.1099/00221287-92-2-263. [DOI] [PubMed] [Google Scholar]
  3. Biggin M. D., Gibson T. J., Hong G. F. Buffer gradient gels and 35S label as an aid to rapid DNA sequence determination. Proc Natl Acad Sci U S A. 1983 Jul;80(13):3963–3965. doi: 10.1073/pnas.80.13.3963. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Birnboim H. C., Doly J. A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Res. 1979 Nov 24;7(6):1513–1523. doi: 10.1093/nar/7.6.1513. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Case M. E., Giles N. H. Evidence for nonsense mutations in the arom gene cluster of Neurospora crassa. Genetics. 1968 Sep;60(1):49–58. doi: 10.1093/genetics/60.1.49. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Catcheside D. E., Storer P. J., Klein B. Cloning of the ARO cluster gene of Neurospora crassa and its expression in Escherichia coli. Mol Gen Genet. 1985;199(3):446–451. doi: 10.1007/BF00330757. [DOI] [PubMed] [Google Scholar]
  7. Clements J. M., Roberts C. F. Molecular cloning of the 3-phosphoglycerate kinase (PGK) gene from Aspergillus nidulans. Curr Genet. 1985;9(4):293–298. doi: 10.1007/BF00419958. [DOI] [PubMed] [Google Scholar]
  8. Dretzen G., Bellard M., Sassone-Corsi P., Chambon P. A reliable method for the recovery of DNA fragments from agarose and acrylamide gels. Anal Biochem. 1981 Apr;112(2):295–298. doi: 10.1016/0003-2697(81)90296-7. [DOI] [PubMed] [Google Scholar]
  9. Gaertner F. H., Cole K. W. A cluster-gene: evidence for one gene, one polypeptide, five enzymes. Biochem Biophys Res Commun. 1977 Mar 21;75(2):259–264. doi: 10.1016/0006-291x(77)91037-3. [DOI] [PubMed] [Google Scholar]
  10. Hawkins A. R., Francisco Da Silva A. J., Roberts C. F. Cloning and characterization of the three enzyme structural genes QUTB, QUTC and QUTE from the quinic acid utilization gene cluster in Aspergillus nidulans. Curr Genet. 1985;9(4):305–311. doi: 10.1007/BF00419960. [DOI] [PubMed] [Google Scholar]
  11. Hawkins A. R., Francisco da Silva A. J., Roberts C. F. Evidence for two control genes regulating expression of the quinic acid utilization (qut) gene cluster in Aspergillus nidulans. J Gen Microbiol. 1984 Mar;130(3):567–574. doi: 10.1099/00221287-130-3-567. [DOI] [PubMed] [Google Scholar]
  12. Hawkins A. R., Giles N. H., Kinghorn J. R. Genetical and biochemical aspects of quinate breakdown in the filamentous fungus Aspergillus nidulans. Biochem Genet. 1982 Apr;20(3-4):271–286. doi: 10.1007/BF00484424. [DOI] [PubMed] [Google Scholar]
  13. Kinghorn J. R., Hawkins A. R. Cloning and expression in Escherichia coli K-12 of the biosynthetic dehydroquinase function of the arom cluster gene from the eucaryote, Aspergillus nidulans. Mol Gen Genet. 1982;186(1):145–152. doi: 10.1007/BF00422927. [DOI] [PubMed] [Google Scholar]
  14. Kinnaird J. H., Fincham J. R. The complete nucleotide sequence of the Neurospora crassa am (NADP-specific glutamate dehydrogenase) gene. Gene. 1983 Dec;26(2-3):253–260. doi: 10.1016/0378-1119(83)90195-6. [DOI] [PubMed] [Google Scholar]
  15. Larimer F. W., Morse C. C., Beck A. K., Cole K. W., Gaertner F. H. Isolation of the ARO1 cluster gene of Saccharomyces cerevisiae. Mol Cell Biol. 1983 Sep;3(9):1609–1614. doi: 10.1128/mcb.3.9.1609. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Lumsden J., Coggins J. R. The subunit structure of the arom multienzyme complex of Neurospora crassa. A possible pentafunctional polypeptide chain. Biochem J. 1977 Mar 1;161(3):599–607. doi: 10.1042/bj1610599. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Matthes H. W., Zenke W. M., Grundström T., Staub A., Wintzerith M., Chambon P. Simultaneous rapid chemical synthesis of over one hundred oligonucleotides on a microscale. EMBO J. 1984 Apr;3(4):801–805. doi: 10.1002/j.1460-2075.1984.tb01888.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Nakanishi N., Yamamoto M. Analysis of the structure and transcription of the aro3 cluster gene in Schizosaccharomyces pombe. Mol Gen Genet. 1984;195(1-2):164–169. doi: 10.1007/BF00332740. [DOI] [PubMed] [Google Scholar]
  19. Patel V. B., Giles N. H. Purification of the arom multienzyme aggregate from Euglena gracilis. Biochim Biophys Acta. 1979 Mar 16;567(1):24–34. doi: 10.1016/0005-2744(79)90168-2. [DOI] [PubMed] [Google Scholar]
  20. Rigby P. W., Dieckmann M., Rhodes C., Berg P. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I. J Mol Biol. 1977 Jun 15;113(1):237–251. doi: 10.1016/0022-2836(77)90052-3. [DOI] [PubMed] [Google Scholar]
  21. Sanger F., Nicklen S., Coulson A. R. DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463–5467. doi: 10.1073/pnas.74.12.5463. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Schechtman M. G., Yanofsky C. Structure of the trifunctional trp-1 gene from Neurospora crassa and its aberrant expression in Escherichia coli. J Mol Appl Genet. 1983;2(1):83–99. [PubMed] [Google Scholar]
  23. Smith D. D., Coggins J. R. Isolation of a bifunctional domain from the pentafunctional arom enzyme complex of Neurospora crassa. Biochem J. 1983 Aug 1;213(2):405–415. doi: 10.1042/bj2130405. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Sproat B. S., Gait M. J. Chemical synthesis of a gene for somatomedin C. Nucleic Acids Res. 1985 Apr 25;13(8):2959–2977. doi: 10.1093/nar/13.8.2959. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Storck R., Alexopoulos C. J. Deoxyribonucleic acid of fungi. Bacteriol Rev. 1970 Jun;34(2):126–154. doi: 10.1128/br.34.2.126-154.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Thomas P. S. Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose. Proc Natl Acad Sci U S A. 1980 Sep;77(9):5201–5205. doi: 10.1073/pnas.77.9.5201. [DOI] [PMC free article] [PubMed] [Google Scholar]

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