Skip to main content
PMC home page
The Journal of Clinical Investigation logoLink to The Journal of Clinical Investigation
. 1969 Jan;48(1):87–95. doi: 10.1172/JCI105977

Effect of mixed micellar lipid on the absorption of cholesterol and vitamin D3 into lymph

Gilbert R Thompson 1, Robert K Ockner 1, Kurt J Isselbacher 1
PMCID: PMC322194  PMID: 4303790

Abstract

The absorption of endogenous cholesterol, labeled with tracer doses of cholesterol 14C or cholesterol-3H and of near physiological doses of vitamin D3-3H was studied in rats with cannulated intestinal lymphatics. The effects of administering mixed micellar solutions of fatty acid, monoglyceride, and bile salt on the absorption of these labeled sterols was determined. It was observed that the specific activity of free cholesterol and the amounts of vitamin D3 appearing in lymph were significantly increased during the intraduodenal administration of mixed micellar solutions of either linoleic or palmitic acid, in contrast to control rats receiving a micellar solution of taurocholate. These increases were related linearly to the lymph triglyceride level. In addition it was observed that when the linoleic acid solution was administered there was a more marked increase in the ratio of the specific activities of free and esterified cholesterol in lymph than with either the palmitic acid or taurocholate solutions.

Additional studies in rats with intact lymphatics showed that the uptake of labeled cholesterol and vitamin D3 from the intestinal lumen into the wall was similar whether the sterols were administered in taurocholate or in mixed micellar solution.

These findings suggest that mixed micellar lipid increased the rate of appearance of labeled free cholesterol and vitamin D3 in lymph by enhancing their transport out of the intestinal mucosa, rather than by an effect on uptake.

Full text

PDF
88

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bell N. H. Comparison of intestinal absorption and esterification of 4-C14 vitamin D3 and 4-C14 cholesterol in the rat. Proc Soc Exp Biol Med. 1966 Nov;123(2):529–532. doi: 10.3181/00379727-123-31534. [DOI] [PubMed] [Google Scholar]
  2. CHAIKOFF I. L., BLOOM B., SIPERSTEIN M. D., KIYASU J. Y., REINHARDT W. O., DAUBEN W. G., EASTHAM J. F. C14 cholesterol. I. Lymphatic transport of absorbed cholesterol-4-C14. J Biol Chem. 1952 Jan;194(1):407–412. [PubMed] [Google Scholar]
  3. DASKALAKIS E. G., CHAIKOFF I. L. The significance of esterification in the absorption of cholesterol from the intestine. Arch Biochem Biophys. 1955 Oct;58(2):373–380. doi: 10.1016/0003-9861(55)90137-5. [DOI] [PubMed] [Google Scholar]
  4. FOLCH J., LEES M., SLOANE STANLEY G. H. A simple method for the isolation and purification of total lipides from animal tissues. J Biol Chem. 1957 May;226(1):497–509. [PubMed] [Google Scholar]
  5. Goodman D. S. Cholesterol ester metabolism. Physiol Rev. 1965 Oct;45(4):747–839. doi: 10.1152/physrev.1965.45.4.747. [DOI] [PubMed] [Google Scholar]
  6. HOFMANN A. F., BORGSTROM B. Physico-chemical state of lipids in intestinal content during their digestion and absorption. Fed Proc. 1962 Jan-Feb;21:43–50. [PubMed] [Google Scholar]
  7. Hofmann A. F., Small D. M. Detergent properties of bile salts: correlation with physiological function. Annu Rev Med. 1967;18:333–376. doi: 10.1146/annurev.me.18.020167.002001. [DOI] [PubMed] [Google Scholar]
  8. JOHNSTON J. M., BORGSTROEM B. THE INTESTINAL ABSORPTION AND METABOLISM OF MICELLAR SOLUTIONS OF LIPIDS. Biochim Biophys Acta. 1964 Aug 5;84:412–423. doi: 10.1016/0926-6542(64)90005-8. [DOI] [PubMed] [Google Scholar]
  9. KARMEN A., WHYTE M., GOODMAN D. S. FATTY ACID ESTERIFICATION AND CHYLOMICRON FORMATION DURING FAT ABSORPTION. 1. TRIGLYCERIDES AND CHOLESTEROL ESTERS. J Lipid Res. 1963 Jul;4:312–321. [PubMed] [Google Scholar]
  10. KIM K. S., IVY A. C. Factors influencing cholesterol absorption. Am J Physiol. 1952 Nov;171(2):302–318. doi: 10.1152/ajplegacy.1952.171.2.302. [DOI] [PubMed] [Google Scholar]
  11. Lund J., DeLuca H. F. Biologically active metabolite of vitamin D3 from bone, liver, and blood serum. J Lipid Res. 1966 Nov;7(6):739–744. [PubMed] [Google Scholar]
  12. PIHL A. On the mode of action of fatty acids in cholesterol absorption. Cholesterol studies. IV. Acta Physiol Scand. 1955 Oct 27;34(2-3):197–205. doi: 10.1111/j.1748-1716.1955.tb01240.x. [DOI] [PubMed] [Google Scholar]
  13. Redgrave T. G. The absorption of micellar lipid into the lymph of unanaesthetised rats. Q J Exp Physiol Cogn Med Sci. 1967 Apr;52(2):130–138. doi: 10.1113/expphysiol.1967.sp001894. [DOI] [PubMed] [Google Scholar]
  14. SCHACHTER D., FINKELSTEIN J. D., KOWARSKI S. METABOLISM OF VITAMIN D. I. PREPARATION OF RADIOACTIVE VITAMIN D AND ITS INTESTINAL ABSORPTION IN THE RAT. J Clin Invest. 1964 May;43:787–796. doi: 10.1172/JCI104965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. SWELL L., FLICK D. F., FIELD H., Jr, TREADWELL C. R. Role of fat and fatty acid in absorption of dietary cholesterol. Am J Physiol. 1955 Jan;180(1):124–128. doi: 10.1152/ajplegacy.1954.180.1.124. [DOI] [PubMed] [Google Scholar]
  16. SWELL L., TROUT E. C., Jr, HOPPER R., FIELD H., Jr, TREADWELL C. R. The mechanism of cholesterol absorption. Ann N Y Acad Sci. 1959 Jun 16;72:813–825. doi: 10.1111/j.1749-6632.1959.tb44207.x. [DOI] [PubMed] [Google Scholar]
  17. Simmonds W. J., Hofmann A. F., Theodor E. Absorption of cholesterol from a micellar solution: intestinal perfusion studies in man. J Clin Invest. 1967 May;46(5):874–890. doi: 10.1172/JCI105587. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Simmonds W. J., Redgrave T. G., Willix R. L. Absorption of oleic and palmitic acids from emulsions and micellar solutions. J Clin Invest. 1968 May;47(5):1015–1025. doi: 10.1172/JCI105791. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Thompson G. R., Lewis B., Booth C. C. Absorption of vitamin D3-3H in control subjects and patients with intestinal malabsorption. J Clin Invest. 1966 Jan;45(1):94–102. doi: 10.1172/JCI105327. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. VAHOUNY G. V., TREADWELL C. R. Comparative effects of dietary fatty acids and triglycerides on lymph lipids in the rat. Am J Physiol. 1959 Apr;196(4):881–883. doi: 10.1152/ajplegacy.1959.196.4.881. [DOI] [PubMed] [Google Scholar]
  21. VAN HANDEL E., ZILVERSMIT D. B. Micromethod for the direct determination of serum triglycerides. J Lab Clin Med. 1957 Jul;50(1):152–157. [PubMed] [Google Scholar]
  22. Wilson J. D., Reinke R. T. Transfer of locally synthesized cholesterol from intestinal wall to intestinal lymph. J Lipid Res. 1968 Jan;9(1):85–92. [PubMed] [Google Scholar]
  23. ZLATKIS A., ZAK B., BOYLE A. J. A new method for the direct determination of serum cholesterol. J Lab Clin Med. 1953 Mar;41(3):486–492. [PubMed] [Google Scholar]
  24. Zilversmit D. B. The composition and structure of lymph chylomicrons in dog, rat, and man. J Clin Invest. 1965 Oct;44(10):1610–1622. doi: 10.1172/JCI105267. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Clinical Investigation are provided here courtesy of American Society for Clinical Investigation

RESOURCES