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. 1969 Oct;48(10):1862–1869. doi: 10.1172/JCI106152

Effect of decreased O2 supply to tissue on the lactate:pyruvate ratio in blood

William A Neill 1, Peter E Jensen 1, Gerald B Rich 1, John D Werschkul 1
PMCID: PMC322422  PMID: 5822591

Abstract

Experiments were performed with trained conscious dogs with permanently implanted intravascular catheters. With the dogs in a basal resting state, the concentrations of lactate (L) and pyruvate (P) in arterial blood fluctuated widely from day to day, whereas their concentration ratio (L/P) remained relatively constant. By contrast, decrease in tissue O2 supply induced by severe chronic anemia increased the arterial blood L/P, specifically, with only random accompanying changes in the lactate or pyruvate concentrations themselves.

When systemic O2 consumption was increased acutely by muscular exercise, cardiac output increased, and the changes in blood L/P were small and not consistent between different dogs. But when O2 supply to the tissues was simultaneously limited by anemia, L/P increased during exercise, and the magnitude of the increase was proportional to the severity of the anemia. These results suggest that changes in blood L/P during exercise are related specifically to tissue O2 supply.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ASMUSSEN E., NIELSEN M. The cardiac output in rest and work determined simultaneously by the acetylene and the dye injection methods. Acta Physiol Scand. 1952;27(2-3):217–230. doi: 10.1111/j.1748-1716.1953.tb00937.x. [DOI] [PubMed] [Google Scholar]
  2. ASTRAND P. O., CUDDY T. E., SALTIN B., STENBERG J. CARDIAC OUTPUT DURING SUBMAXIMAL AND MAXIMAL WORK. J Appl Physiol. 1964 Mar;19:268–274. doi: 10.1152/jappl.1964.19.2.268. [DOI] [PubMed] [Google Scholar]
  3. Alpert N. R. Lactate production and removal and the regulation of metabolism. Ann N Y Acad Sci. 1965 Jul 31;119(3):995–1012. doi: 10.1111/j.1749-6632.1965.tb47457.x. [DOI] [PubMed] [Google Scholar]
  4. BAILIE M. D., ROBINSON S., ROSTORFER H. H., NEWTON J. L. Effects of exercise on heart output of the dog. J Appl Physiol. 1961 Jan;16:107–111. doi: 10.1152/jappl.1961.16.1.107. [DOI] [PubMed] [Google Scholar]
  5. BARGER A. C., RICHARDS V., METCALFE J., GUNTHER B. Regulation of the circulation during exercise; cardiac output (direct Fick) and metabolic adjustments in the normal dog. Am J Physiol. 1956 Mar;184(3):613–623. doi: 10.1152/ajplegacy.1956.184.3.613. [DOI] [PubMed] [Google Scholar]
  6. BARLOW G., KNOTT D. H. HEMODYNAMIC ALTERATIONS AFTER 30 MINUTES OF PENTOBARBITAL SODIUM ANESTHESIA IN DOGS. Am J Physiol. 1964 Oct;207:764–766. doi: 10.1152/ajplegacy.1964.207.4.764. [DOI] [PubMed] [Google Scholar]
  7. CARLSON L. A., PERNOW B. Studies on the peripheral circulation and metabolism in man. 1. Oxygen utilization and lactate-pyruvate formation in the legs at rest and during exercise in healthy subjects. Acta Physiol Scand. 1961 Jul-Aug;52:328–342. doi: 10.1111/j.1748-1716.1961.tb02229.x. [DOI] [PubMed] [Google Scholar]
  8. CARLSON L. A., PERNOW B. Studies on the peripheral circulation and metabolism in man. II. Oxygen utilization and lactate-pyruvate formation in the legs at rest and during exercise in patients with arteriosclerosis obliterans. Acta Med Scand. 1962 Mar;171:311–323. [PubMed] [Google Scholar]
  9. CHAPMAN C. B., FISHER J. N., SPROULE B. J. Behavior of stroke volume at rest and during exercise in human beings. J Clin Invest. 1960 Aug;39:1208–1213. doi: 10.1172/JCI104136. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. COBB L. A., JOHNSON W. P. Hemodynamic relationships of anaerobic metabolism and plasma free fatty acids during prolonged, strenuous exercise in trained and untrained subjects. J Clin Invest. 1963 Jun;42:800–810. doi: 10.1172/JCI104772. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Cain S. M. Appearance of excess lactate in anesthetized dogs during anemic and hypoxic hypoxia. Am J Physiol. 1965 Sep;209(3):604–610. doi: 10.1152/ajplegacy.1965.209.3.604. [DOI] [PubMed] [Google Scholar]
  12. Edhag O., Zetterquist S. Peripheral circulatory adaptation to exercise in restricted cardiac output. A hemodynamic and metabolic study in patients with complete heart block and artificial pacemaker. Scand J Clin Lab Invest. 1968;21(2):123–135. doi: 10.3109/00365516809084274. [DOI] [PubMed] [Google Scholar]
  13. Eldridge F. Blood lactate and pyruvate in pulmonary insufficiency. N Engl J Med. 1966 Apr 21;274(16):878–883. doi: 10.1056/NEJM196604212741604. [DOI] [PubMed] [Google Scholar]
  14. GLICK G., PLAUTH W. H., Jr, BRAUNWALD E. ROLE OF THE AUTONOMIC NERVOUS SYSTEM IN THE CIRCULATORY RESPONSE TO ACUTELY INDUCED ANEMIA IN UNANESTHETIZED DOGS. J Clin Invest. 1964 Nov;43:2112–2124. doi: 10.1172/JCI105085. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. GREENE N. M., TALNER N. S. BLOOD LACTATE, PYRUVATE AND LACTATE-PYRUVATE RATIOS IN CONGENITAL HEART DISEASE. N Engl J Med. 1964 Jun 18;270:1331–1336. doi: 10.1056/NEJM196406182702502. [DOI] [PubMed] [Google Scholar]
  16. GUDBJARNASON S., BING R. J. The redox-potential of the lactate-pyruvate system in blood as an indicator of the functional state of cellular oxidation. Biochim Biophys Acta. 1962 Jun 18;60:158–162. doi: 10.1016/0006-3002(62)90382-7. [DOI] [PubMed] [Google Scholar]
  17. HARRIS P., BATEMAN M., GLOSTER J. Relations between the cardio-respiratory effects of exercise and the arterial concentration of lactate and pyruvate in patients with rheumatic heart disease. Clin Sci. 1962 Dec;23:531–543. [PubMed] [Google Scholar]
  18. HAVEL R. J., WATKINS E., Jr The metabolism of lactate and pyruvate in children with congenital heart disease. Circulation. 1950 Oct;2(4):536–544. doi: 10.1161/01.cir.2.4.536. [DOI] [PubMed] [Google Scholar]
  19. HUCKABEE W. E. Control of concentration gradients of pyruvate and lactate across cell membranes in blood. J Appl Physiol. 1956 Sep;9(2):163–170. doi: 10.1152/jappl.1956.9.2.163. [DOI] [PubMed] [Google Scholar]
  20. HUCKABEE W. E., JUDSON W. E. The role of anaerobic metabolism in the performance of mild muscular work. I. Relationship to oxygen consumption and cardiac output, and the effect of congestive heart failure. J Clin Invest. 1958 Nov;37(11):1577–1592. doi: 10.1172/JCI103751. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. HUCKABEE W. E. METABOLIC CONSEQUENCES OF CHRONIC HYPOXIA. Ann N Y Acad Sci. 1965 Mar 24;121:723–730. doi: 10.1111/j.1749-6632.1965.tb14240.x. [DOI] [PubMed] [Google Scholar]
  22. HUCKABEE W. E. Relationships of pyruvate and lactate during anaerobic metabolism. I. Effects of infusion of pyruvate or glucose and of hyperventilation. J Clin Invest. 1958 Feb;37(2):244–254. doi: 10.1172/JCI103603. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. HUCKABEE W. E. Relationships of pyruvate and lactate during anaerobic metabolism. III. Effect of breathing low-oxygen gases. J Clin Invest. 1958 Feb;37(2):264–271. doi: 10.1172/JCI103605. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Hlavová A., Linhart J., Prerovský I., Ganz V. Lactate and pyruvate changes in the leg during and after exercise in normal subjects and in patients with femoral artery occlusion. Clin Sci. 1968 Jun;34(3):397–409. [PubMed] [Google Scholar]
  25. KNUTTGEN H. G. Oxygen debt, lactate, pyruvate, and excess lactate after muscular work. J Appl Physiol. 1962 Jul;17:639–644. doi: 10.1152/jappl.1962.17.4.639. [DOI] [PubMed] [Google Scholar]
  26. Neill W. A., Huckabee W. E. Anaerobic heat production by the heart. J Clin Invest. 1966 Sep;45(9):1412–1420. doi: 10.1172/JCI105449. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Neill W. A. Myocardial hypoxia and anerobic metabolism in coronary heart disease. Am J Cardiol. 1968 Oct;22(4):507–515. doi: 10.1016/0002-9149(68)90155-0. [DOI] [PubMed] [Google Scholar]
  28. OLSON R. E. "EXCESS LACTATE" AND ANAEROBIOSIS. Ann Intern Med. 1963 Dec;59:960–963. doi: 10.7326/0003-4819-59-6-960. [DOI] [PubMed] [Google Scholar]
  29. SALTIN B. CIRCULATORY RESPONSE TO SUBMAXIMAL AND MAXIMAL EXERCISE AFTER THERMAL DEHYDRATION. J Appl Physiol. 1964 Nov;19:1125–1132. doi: 10.1152/jappl.1964.19.6.1125. [DOI] [PubMed] [Google Scholar]
  30. Seibert D. J., Ebaugh F. G., Jr Assessment of tissue anoxemia in chronic anemia by the arterial lactate/pyruvate ratio and excess lactate formation. J Lab Clin Med. 1967 Feb;69(2):177–182. [PubMed] [Google Scholar]

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