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. 1970 Oct;49(10):1912–1921. doi: 10.1172/JCI106410

A new method for the measurement of thyroidal iodine release in man

John T Nicoloff 1
PMCID: PMC322681  PMID: 5456801

Abstract

A new method is described for the detection of variations in the release rate of all iodinated products by the thyroid gland in man. 125I was employed to endogenously label the thyroid gland, and a parenterally administered 131I thyroxine dose was used to generate a 131I reference source. Thyroidal iodine release activity was quantitatively assessed by the measurement of variations in 125I/131I ratio values obtained in timed urine samples. Diurnal variation in thyroid release patterns was observed in euthyroid subjects which was promptly suppressed by exogenous triiodothyronine administration and was simulated by the intramuscular injection of 0.25-0.50 U of bovine thyrotropin. The zenith value occurred at 4:00 a.m. ±3.4 hr (SD) and the nadir at 5:00 p.m. ±3.6 hr. The absence of a diurnal pattern in patients with thyrotoxicosis and in secondary hypothyroidism indicated that this diurnal fluctuation was under thyrotropin (TSH) regulation. This new method also promises to be a useful tool for the study of the intrathyroidal recycling of iodide from the iodotyrosine pool and the detection of factors which may acutely alter thyroid function.

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1920

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ANDERSSON B., EKMAN L., GALE C. C., SUNDSTEN J. W. CONTROL OF THYROTROPHIC HORMONE (TSH) SECRETION BY THE "HEAT LOSS CENTER". Acta Physiol Scand. 1963 Sep-Oct;59:12–33. doi: 10.1111/j.1748-1716.1963.tb02719.x. [DOI] [PubMed] [Google Scholar]
  2. Buhler U. K., DeGroot L. J. Effect of stable iodine on thyroid iodine release. J Clin Endocrinol Metab. 1969 Dec;29(12):1546–1552. doi: 10.1210/jcem-29-12-1546. [DOI] [PubMed] [Google Scholar]
  3. Charters A. C., Odell W. D., Thompson J. C. Anterior pituitary function during surgical stress and convalescence. Radioimmunoassay measurement of blood TSH, LH, FSH and growth hormone. J Clin Endocrinol Metab. 1969 Jan;29(1):63–71. doi: 10.1210/jcem-29-1-63. [DOI] [PubMed] [Google Scholar]
  4. Ducommun P., Sakiz E., Guillemin R. Dissociation of the acute secretions of thyrotropin and adrenocorticotropin. Am J Physiol. 1966 Jun;210(6):1257–1259. doi: 10.1152/ajplegacy.1966.210.6.1257. [DOI] [PubMed] [Google Scholar]
  5. Fore W., Kohler P., Wynn J. Rapid redistribution of serum thyroxine during ether anesthesia. J Clin Endocrinol Metab. 1966 Aug;26(8):821–830. doi: 10.1210/jcem-26-8-821. [DOI] [PubMed] [Google Scholar]
  6. Greer M. A., Grimm Y., Inoue K. Fate of iodide derived from intrathyroidal hyrolysis of thyroglobulin. Endocrinology. 1969 Nov;85(5):837–845. doi: 10.1210/endo-85-5-837. [DOI] [PubMed] [Google Scholar]
  7. Hays M. T., Solomon D. H. Effect of epinephrine on the peripheral metabolism of thyroxine. J Clin Invest. 1969 Jun;48(6):1114–1123. doi: 10.1172/JCI106068. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. INGBAR S. H., GALTON V. A. Thyroid. Annu Rev Physiol. 1963;25:361–384. doi: 10.1146/annurev.ph.25.030163.002045. [DOI] [PubMed] [Google Scholar]
  9. Irvine C. H. Effect of exercise on thyroxine degradation in athletes and non-athletes. J Clin Endocrinol Metab. 1968 Jul;28(7):942–948. doi: 10.1210/jcem-28-7-942. [DOI] [PubMed] [Google Scholar]
  10. Isaacs G. H., Athans J. C., Rosenberg I. N. Effects of thyrotropin upon thyroidal iodide; studies using thyroid venous cannulation and two radioiodine isotopes. J Clin Invest. 1966 May;45(5):758–767. doi: 10.1172/JCI105390. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Lemarchand-Béraud T., Vannotti A. Relationships between blood thyrotrophin level, protein bound iodine and free thyroxine concentration in man under normal physiological conditions. Acta Endocrinol (Copenh) 1969 Feb;60(2):315–326. doi: 10.1530/acta.0.0600315. [DOI] [PubMed] [Google Scholar]
  12. Nagataki S., Shizume K., Nakao K. Thyroid function in chronic excess iodide ingestion: release of inorganic iodide from thyroid gland in response to chronic iodide administration. Endocrinology. 1969 Nov;85(5):899–908. doi: 10.1210/endo-85-5-899. [DOI] [PubMed] [Google Scholar]
  13. Nicoloff J. T. A new method for the measurement of acute alterations in thyroxine deiodination rate in man. J Clin Invest. 1970 Feb;49(2):267–273. doi: 10.1172/JCI106236. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Nicoloff J. T., Dowling J. T. Estimation of thyroxine distribution in man. J Clin Invest. 1968 Jan;47(1):26–37. doi: 10.1172/JCI105712. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Ohtaki S., Moriya S., Suzuki H., Moriuchi Y. Nonhormonal iodine escape from the normal and abnormal thyroid gland. J Clin Endocrinol Metab. 1967 May;27(5):728–740. doi: 10.1210/jcem-27-5-728. [DOI] [PubMed] [Google Scholar]
  16. SCHNEIDER P. B. THYROIDAL IODINE HETEROGENEITY: "LAST COME, FIRST SERVED" SYSTEM OF IODINE TURNOVER. Endocrinology. 1964 Jun;74:973–980. doi: 10.1210/endo-74-6-973. [DOI] [PubMed] [Google Scholar]
  17. STANBURY J. B., KASSENAAR A. A., MEIJER J. W. The metabolism of iodotyrosines. I. The fate of mono- and di-iodotyrosine in normal subjects and in patients with various diseases. J Clin Endocrinol Metab. 1956 Jun;16(6):735–746. doi: 10.1210/jcem-16-6-735. [DOI] [PubMed] [Google Scholar]
  18. Singh D. V., Panda J. N., Anderson R. R., Turner C. W. Diurnal variation of plasma and pituitary thyrotropin (TSH) of rats. Proc Soc Exp Biol Med. 1967 Nov;126(2):553–554. doi: 10.3181/00379727-126-32502. [DOI] [PubMed] [Google Scholar]
  19. Suzuki M., Tonoue T., Matsuzaki S., Yamamoto K. Initial response of human thyroid, adrenal cortex, and adrenal medulla to acute cold exposure. Can J Physiol Pharmacol. 1967 May;45(3):423–432. doi: 10.1139/y67-051. [DOI] [PubMed] [Google Scholar]
  20. UTIGER R. D. RADIOIMMUNOASSAY OF HUMAN PLASMA THYROTROPIN. J Clin Invest. 1965 Aug;44:1277–1286. doi: 10.1172/JCI105234. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Wolff J. Iodide goiter and the pharmacologic effects of excess iodide. Am J Med. 1969 Jul;47(1):101–124. doi: 10.1016/0002-9343(69)90245-9. [DOI] [PubMed] [Google Scholar]
  22. Yamamoto Y., Moriya S., Horiuchi Y. Iodide outflow from thyroid: its relation to plasma inorganic iodide concentration. J Clin Endocrinol Metab. 1969 Feb;29(2):265–272. doi: 10.1210/jcem-29-2-265. [DOI] [PubMed] [Google Scholar]

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