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. 1986 Jan;83(2):366–368. doi: 10.1073/pnas.83.2.366

Introduction of genes into preimplantation mouse embryos by use of a defective recombinant retrovirus.

J L Rubenstein, J F Nicolas, F Jacob
PMCID: PMC322859  PMID: 3455774

Abstract

Two-cell and four-cell preimplantation mouse embryos were cocultured in vitro with fibroblasts producing the recombinant retrovirus M-MuLV-neo. No wildtype helper virus was detected in these cultures. Of the embryos that survived the in vitro cultivation and the reimplantation into foster mothers, 2 of 15 that were tested contained the proviral genome. The provirus integrated as a single copy at a unique site. We estimate that approximately equal to 20% of the cells in each of the two transgenic fetuses contained the provirus.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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