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Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1986 Mar;83(5):1383–1387. doi: 10.1073/pnas.83.5.1383

Evolutionary diversity of eukaryotic small-subunit rRNA genes.

M L Sogin, H J Elwood, J H Gunderson
PMCID: PMC323080  PMID: 2419907

Abstract

The small-subunit rRNA gene sequences of the flagellated protists Euglena gracilis and Trypanosoma brucei were determined and compared to those of other eukaryotes. A phylogenetic tree was constructed in which the earliest branching among the eukaryotes is represented by E. gracilis. The E. gracilis divergence far antedates a period of massive evolutionary radiation that gave rise to the plants, animals, fungi, and certain groups of protists such as ciliates and the acanthamoebae. The genetic diversity in this collection of eukaryotes is seen to exceed that displayed within either the eubacterial or the archaebacterial lines of descent.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Brosius J., Palmer M. L., Kennedy P. J., Noller H. F. Complete nucleotide sequence of a 16S ribosomal RNA gene from Escherichia coli. Proc Natl Acad Sci U S A. 1978 Oct;75(10):4801–4805. doi: 10.1073/pnas.75.10.4801. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Cavalier-tsmith T. The origin of nuclei and of eukaryotic cells. Nature. 1975 Aug 7;256(5517):463–468. doi: 10.1038/256463a0. [DOI] [PubMed] [Google Scholar]
  3. Chan Y. L., Gutell R., Noller H. F., Wool I. G. The nucleotide sequence of a rat 18 S ribosomal ribonucleic acid gene and a proposal for the secondary structure of 18 S ribosomal ribonucleic acid. J Biol Chem. 1984 Jan 10;259(1):224–230. [PubMed] [Google Scholar]
  4. Connaughton J. F., Rairkar A., Lockard R. E., Kumar A. Primary structure of rabbit 18S ribosomal RNA determined by direct RNA sequence analysis. Nucleic Acids Res. 1984 Jun 11;12(11):4731–4745. doi: 10.1093/nar/12.11.4731. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Corliss J. O. The kingdom Protista and its 45 phyla. Biosystems. 1984;17(2):87–126. doi: 10.1016/0303-2647(84)90003-0. [DOI] [PubMed] [Google Scholar]
  6. Curtis S. E., Rawson J. R. Characterization of the nuclear ribosomal DNA of Euglena gracilis. Gene. 1981 Nov;15(2-3):237–247. doi: 10.1016/0378-1119(81)90133-5. [DOI] [PubMed] [Google Scholar]
  7. Elwood H. J., Olsen G. J., Sogin M. L. The small-subunit ribosomal RNA gene sequences from the hypotrichous ciliates Oxytricha nova and Stylonychia pustulata. Mol Biol Evol. 1985 Sep;2(5):399–410. doi: 10.1093/oxfordjournals.molbev.a040362. [DOI] [PubMed] [Google Scholar]
  8. Fitch W. M., Margoliash E. Construction of phylogenetic trees. Science. 1967 Jan 20;155(3760):279–284. doi: 10.1126/science.155.3760.279. [DOI] [PubMed] [Google Scholar]
  9. Fitch W. M. The molecular evolution of cytochrome c in eukaryotes. J Mol Evol. 1976 Jun 23;8(1):13–40. doi: 10.1007/BF01738880. [DOI] [PubMed] [Google Scholar]
  10. Fox G. E., Stackebrandt E., Hespell R. B., Gibson J., Maniloff J., Dyer T. A., Wolfe R. S., Balch W. E., Tanner R. S., Magrum L. J. The phylogeny of prokaryotes. Science. 1980 Jul 25;209(4455):457–463. doi: 10.1126/science.6771870. [DOI] [PubMed] [Google Scholar]
  11. Gray M. W., Doolittle W. F. Has the endosymbiont hypothesis been proven? Microbiol Rev. 1982 Mar;46(1):1–42. doi: 10.1128/mr.46.1.1-42.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Gupta R., Lanter J. M., Woese C. R. Sequence of the 16S Ribosomal RNA from Halobacterium volcanii, an Archaebacterium. Science. 1983 Aug 12;221(4611):656–659. doi: 10.1126/science.221.4611.656. [DOI] [PubMed] [Google Scholar]
  13. Hasan G., Turner M. J., Cordingley J. S. Ribosomal RNA genes of Trypanosoma brucei. Cloning of a rRNA gene containing a mobile element. Nucleic Acids Res. 1982 Nov 11;10(21):6747–6761. doi: 10.1093/nar/10.21.6747. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Hori H., Osawa S. Evolutionary change in 5S RNA secondary structure and a phylogenic tree of 54 5S RNA species. Proc Natl Acad Sci U S A. 1979 Jan;76(1):381–385. doi: 10.1073/pnas.76.1.381. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Lumsden J., Hall D. O. Superoxide dismutase in photosynthetic organisms provides an evolutionary hypothesis. Nature. 1975 Oct 23;257(5528):670–672. doi: 10.1038/257670a0. [DOI] [PubMed] [Google Scholar]
  16. McLaughlin P. J., Dayhoff M. O. Eukaryote evolution: a view based on cytochrome c sequence data. J Mol Evol. 1973;2(2-3):99–116. doi: 10.1007/BF01653990. [DOI] [PubMed] [Google Scholar]
  17. Messing J., Carlson J., Hagen G., Rubenstein I., Oleson A. Cloning and sequencing of the ribosomal RNA genes in maize: the 17S region. DNA. 1984;3(1):31–40. doi: 10.1089/dna.1.1984.3.31. [DOI] [PubMed] [Google Scholar]
  18. Messing J. New M13 vectors for cloning. Methods Enzymol. 1983;101:20–78. doi: 10.1016/0076-6879(83)01005-8. [DOI] [PubMed] [Google Scholar]
  19. Nelles L., Fang B. L., Volckaert G., Vandenberghe A., De Wachter R. Nucleotide sequence of a crustacean 18S ribosomal RNA gene and secondary structure of eukaryotic small subunit ribosomal RNAs. Nucleic Acids Res. 1984 Dec 11;12(23):8749–8768. doi: 10.1093/nar/12.23.8749. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Ohama T., Kumazaki T., Hori H., Osawa S. Evolution of multicellular animals as deduced from 5S rRNA sequences: a possible early emergence of the Mesozoa. Nucleic Acids Res. 1984 Jun 25;12(12):5101–5108. doi: 10.1093/nar/12.12.5101. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Olsen G. J., Sogin M. L. Nucleotide sequence of Dictyostelium discoideum 5.8S ribosomal ribonucleic acid: evolutionary and secondary structural implications. Biochemistry. 1982 May 11;21(10):2335–2343. doi: 10.1021/bi00539a010. [DOI] [PubMed] [Google Scholar]
  22. Rawson J. R., Stutz E. Characterization of Euglena cytoplasmic ribosomes and ribosomal RNA by zone velocity sedimentation IN SUCROSE GRADIENTS. J Mol Biol. 1968 Apr 14;33(1):309–314. doi: 10.1016/0022-2836(68)90296-9. [DOI] [PubMed] [Google Scholar]
  23. Rubtsov P. M., Musakhanov M. M., Zakharyev V. M., Krayev A. S., Skryabin K. G., Bayev A. A. The structure of the yeast ribosomal RNA genes. I. The complete nucleotide sequence of the 18S ribosomal RNA gene from Saccharomyces cerevisiae. Nucleic Acids Res. 1980 Dec 11;8(23):5779–5794. doi: 10.1093/nar/8.23.5779. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Salim M., Maden B. E. Nucleotide sequence of Xenopus laevis 18S ribosomal RNA inferred from gene sequence. Nature. 1981 May 21;291(5812):205–208. doi: 10.1038/291205a0. [DOI] [PubMed] [Google Scholar]
  25. Sanger F., Coulson A. R. A rapid method for determining sequences in DNA by primed synthesis with DNA polymerase. J Mol Biol. 1975 May 25;94(3):441–448. doi: 10.1016/0022-2836(75)90213-2. [DOI] [PubMed] [Google Scholar]
  26. Spangler E. A., Blackburn E. H. The nucleotide sequence of the 17S ribosomal RNA gene of Tetrahymena thermophila and the identification of point mutations resulting in resistance to the antibiotics paromomycin and hygromycin. J Biol Chem. 1985 May 25;260(10):6334–6340. [PubMed] [Google Scholar]
  27. Takaiwa F., Oono K., Sugiura M. The complete nucleotide sequence of a rice 17S rRNA gene. Nucleic Acids Res. 1984 Jul 11;12(13):5441–5448. doi: 10.1093/nar/12.13.5441. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Tomioka N., Sugiura M. The complete nucleotide sequence of a 16S ribosomal RNA gene from a blue-green alga, Anacystis nidulans. Mol Gen Genet. 1983;191(1):46–50. doi: 10.1007/BF00330888. [DOI] [PubMed] [Google Scholar]
  29. Williams B. G., Blattner F. R. Construction and characterization of the hybrid bacteriophage lambda Charon vectors for DNA cloning. J Virol. 1979 Feb;29(2):555–575. doi: 10.1128/jvi.29.2.555-575.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Yang D., Oyaizu Y., Oyaizu H., Olsen G. J., Woese C. R. Mitochondrial origins. Proc Natl Acad Sci U S A. 1985 Jul;82(13):4443–4447. doi: 10.1073/pnas.82.13.4443. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. Zuckerkandl E., Pauling L. Molecules as documents of evolutionary history. J Theor Biol. 1965 Mar;8(2):357–366. doi: 10.1016/0022-5193(65)90083-4. [DOI] [PubMed] [Google Scholar]

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