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. 1986 Mar;83(6):1573–1577. doi: 10.1073/pnas.83.6.1573

Bovine galactosyltransferase: identification of a clone by direct immunological screening of a cDNA expression library.

N L Shaper, J H Shaper, J L Meuth, J L Fox, H Chang, I R Kirsch, G F Hollis
PMCID: PMC323125  PMID: 2419911

Abstract

A 1.3-kilobase cDNA clone (7A) coding for bovine galactosyltransferase (glycoprotein 4-beta-galactosyltransferase, EC 2.4.1.38) was isolated from a lambda gt11 expression library by immunological screening with monospecific polyclonal antisera to the affinity-purified bovine enzyme. The nucleotide sequence of this clone predicts an open reading frame that starts at the 5' end of the insert and codes for a polypeptide of 334 amino acids with Mr 37,645. Based on a Mr of 57,000 for the membrane-bound enzyme this clone accounts for approximately 61% of the coding sequence. Portions of the predicted amino acid sequence matched the six tryptic peptides isolated from affinity-purified bovine galactosyltransferase. Clone 7A hybridizes to a 4.8-kilobase bovine mRNA and identifies multiple EcoRI restriction fragments in bovine, murine, and human DNA.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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