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. 1986 Apr;83(7):2037–2041. doi: 10.1073/pnas.83.7.2037

Nucleotide trapping at the ATPase site of myosin subfragment 1 by a new interthiol crosslinking.

P Chaussepied, D Mornet, R Kassab
PMCID: PMC323225  PMID: 2938184

Abstract

When myosin subfragment 1 derivatives in which the reactive sulfhydryl SH1 has been blocked react with N,N'-p-phenylenedimaleimide or 5,5'-dithiobis(2-nitrobenzoic acid), the reactive sulfhydryl group SH2 of the 20-kDa domain is crosslinked with a thiol of the 50-kDa domain of the heavy chain. The crosslink induces the stable trapping of a significant amount of Mg2+-nucleotide in the ATPase site.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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