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. 1980 Apr 11;8(7):1561–1573. doi: 10.1093/nar/8.7.1561

Construction and characterization of a cDNA clone containing a portion of the bovine prolactin sequence.

J H Nilson, A R Thomason, S Horowitz, N L Sasavage, J Blenis, R Albers, W Salser, F M Rottman
PMCID: PMC324017  PMID: 7001369

Abstract

Poly(A)-containing RNA from the bovine anterior pituitary has been used as a template for the enzymatic synthesis of double-stranded cDNA. The resulting double-stranded cDNA was inserted into the Pst I site of pBR322 with the oligo(dG)-oligo(dC) tailing technique and subsequently cloned in E. coli chi 1776. Clones containing sequences complementary to prolactin mRNA were identified by colony hybridization with partially purified prolactin cDNA. A 250 base pair sequence from one prolactin positive clone was extensively characterized and shown to contain the coding information for amino acids 119-192 of authentic bovine prolactin. The recombinant DNA from this clone was covalently attached to diazotized aminocellulose and used to purify prolactin mRNA from a mixture of mRNAs.

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Selected References

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