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. 1980 Jun 11;8(11):2413–2429. doi: 10.1093/nar/8.11.2413

DNA-like duplexes with repetitions. I. Properties of concatemer duplexes formed by d(T-G-C-A-C-A-T-G).

Z A Shabarova, N G Dolinnaya, S I Turkin, E S Gromova
PMCID: PMC324090  PMID: 7443510

Abstract

A new class of synthetic DNA duplexes containing repeating oligonucleotide sequences, double-helical concatemers, is characterized. The UV-absorption and circular dichroism of a concatemer formed in self-association of d(T-G-C-A-C-A-T-G) have been studied. The thermodynamical parameters of complex formation are the following: delta Ho1 = -9.2 +/- 0.3 kcal/mol, delta So1 = -27 +/- 1 e.u. The data obtained show that pseudopolymeric duplexes having structures that are similar to DNA-B-type helices are formed in solutions of d(T-G-C-A-C-A-T-G). Polymerization of 32P-d(pT-G-C-A-C-A-T-G) induced by water-soluble carbodiimide has been carried out under the conditions of concatemer stability. The yield of the dimer, a 16-member oligonucleotide, was 13%.

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Selected References

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