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. 1980 Jun 11;8(11):2527–2535. doi: 10.1093/nar/8.11.2527

A new conformation-specific infrared band of A-DNA in films.

W Pohle, H Fritzsche
PMCID: PMC324099  PMID: 7443513

Abstract

A band at 1185 cm-1 occurs in the infrared spectra of nucleic acids in their A-type conformation which has been reproduced in the literature, too. The absorbance of this band is proportional to the fraction of the A form of DNA samples containing a mixture of different forms. The 1185 cm-1 band has been assigned tentatively to a vibration of the sugar-phosphate backbone with a fairly high contribution from the sugar moiety. Drastic dehydration of the DNA films is accompanied by a continuous intensity decrease of the 1185 cm-1 band indicating a collapse of the A form.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Arnott S., Hukins D. W. Optimised parameters for A-DNA and B-DNA. Biochem Biophys Res Commun. 1972 Jun 28;47(6):1504–1509. doi: 10.1016/0006-291x(72)90243-4. [DOI] [PubMed] [Google Scholar]
  2. Brahms J., Pilet J., Phuong Lan T. T., Hill L. R. Direct evidence of the C-like form of sodium deoxyribonucleate. Proc Natl Acad Sci U S A. 1973 Dec;70(12):3352–3355. doi: 10.1073/pnas.70.12.3352. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Champeil P., Tran T. P., Brahms J. A new approach to the characterization of the B and A forms of DNA by I.R. spectroscopy. Biochem Biophys Res Commun. 1973 Dec 10;55(3):881–887. doi: 10.1016/0006-291x(73)91226-6. [DOI] [PubMed] [Google Scholar]
  4. Erfurth S. C., Kiser E. J., Peticolas W. L. Determination of the backbone structure of nucleic acids and nucleic acid oligomers by laser Raman scattering. Proc Natl Acad Sci U S A. 1972 Apr;69(4):938–941. doi: 10.1073/pnas.69.4.938. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Fritzsche H., Lang H., Pohle W. Evidence for B-C transition in ultraviolet-irradiated DNA. An infrared linear dichroism study. Biochim Biophys Acta. 1976 May 19;432(3):409–412. doi: 10.1016/0005-2787(76)90153-2. [DOI] [PubMed] [Google Scholar]
  6. Higuchi S., Tsuboi M., Iitaka Y. Infrared spectrum of a DNA-RNA hybrid. Biopolymers. 1969;7(6):909–916. doi: 10.1002/bip.1969.360070606. [DOI] [PubMed] [Google Scholar]
  7. Kursar T., Holzwarth G. Backbone conformational change in the A to B Transition of deoxyribonucleic acid. Biochemistry. 1976 Jul 27;15(15):3352–3357. doi: 10.1021/bi00660a028. [DOI] [PubMed] [Google Scholar]
  8. Liquier J., Gadenne M. C., Taillandier E., Defer N., Favatier F., Kruh J. Conformation of DNA in chromatin protein-DNA complexes studied by infrared spectroscopy. Nucleic Acids Res. 1979 Apr;6(4):1479–1493. doi: 10.1093/nar/6.4.1479. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Liquier J., Pinot-Lafaix M., Taillandier E., Brahms J. Infrared linear dichroism investigations of deoxyribonucleic acid complexes with poly(L-arginine) and poly(L-lysine). Biochemistry. 1975 Sep 23;14(19):4191–4197. doi: 10.1021/bi00690a007. [DOI] [PubMed] [Google Scholar]
  10. Liquier J., Taboury J., Taillandier E., Brahms J. Infrared linear dichroism investigations of deoxyribonucleic complexes with histones H2B and H3. Biochemistry. 1977 Jul 12;16(14):3262–3266. doi: 10.1021/bi00633a034. [DOI] [PubMed] [Google Scholar]
  11. Lu K. C., Prohofsky E. W., Van Zandt L. L. Vibrational modes of A-DNA, B-DNA, and A-RNA backbones: an application of a green-function refinement procedure. Biopolymers. 1977 Nov;16(11):2491–2506. doi: 10.1002/bip.1977.360161112. [DOI] [PubMed] [Google Scholar]
  12. Pilet J., Blicharski J., Brahms J. Conformations and structural transitions in polydeoxynucleotides. Biochemistry. 1975 May 6;14(9):1869–1876. doi: 10.1021/bi00680a011. [DOI] [PubMed] [Google Scholar]
  13. Sato T., Kyogoku Y., Higuchi S., Mitsui Y., Iitaka Y., Tsuboi M., Miura K. I. A preliminay investigation on the molecular structure of rice dwarf virus ribonucleic acid. J Mol Biol. 1966 Mar;16(1):180–190. doi: 10.1016/s0022-2836(66)80271-1. [DOI] [PubMed] [Google Scholar]
  14. Shie M., Kharitonenkov I. G., Tikhonenko T. I., Chirgadze Y. N. New possibilities of investigating nucleic acids and nucleoproteins in aqueous solutions by infrared spectroscopy. Nature. 1972 Feb 18;235(5338):386–388. doi: 10.1038/235386a0. [DOI] [PubMed] [Google Scholar]
  15. Taillandier E., Taboury J., Liquier J., Gadenne M. C., Champagne M., Brahms J. Particular structural role of H1 in complexes with DNA and comparison with H2A- and H4-DNA complexes investigated by IR linear dichroism. Biopolymers. 1979 Aug;18(8):1877–1888. doi: 10.1002/bip.1979.360180805. [DOI] [PubMed] [Google Scholar]
  16. Thomas G. J., Jr Raman spectral studies of nucleic acids. 3. Laser-excited spectra of ribosomal RNA. Biochim Biophys Acta. 1970 Aug 8;213(2):417–423. doi: 10.1016/0005-2787(70)90049-3. [DOI] [PubMed] [Google Scholar]

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