Skip to main content
PMC home page
Nucleic Acids Research logoLink to Nucleic Acids Research
. 1980 Jun 25;8(12):2771–2782. doi: 10.1093/nar/8.12.2771

Distinction between mouse DNA polymerases alpha and beta by tryptic peptide mapping.

S R Planck, K Tanabe, S H Wilson
PMCID: PMC324119  PMID: 7433094

Abstract

Results presented here and in a previous paper (Tanabe et al. (1979) Biochemistry 18, 3401--3406) indicate that mouse beta-polymerase is a single polypeptide with an apparent molecular weight of 40,000. This polypeptide has now been analyzed by tryptic peptide mapping. Comparison of the results with identical analysis of mouse alpha-polymerase reveals that the tryptic peptides derived from the two enzymes are different. These results indicate that beta-polymerase is neither a subunit of alpha-polymerase nor a proteolytic degradation product of alpha-polymerase.

Full text

PDF
2775

Images in this article

2775Image
on p.2775
2778Image
on p.2778
2779Image
on p.2779

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Andrews P. Estimation of the molecular weights of proteins by Sephadex gel-filtration. Biochem J. 1964 May;91(2):222–233. doi: 10.1042/bj0910222. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bandyopadhyay A. K. Purification and properties of nuclear and cytoplasmic DNA polymerases from JLS-V9 cells. Arch Biochem Biophys. 1975 Jan;166(1):72–82. doi: 10.1016/0003-9861(75)90367-7. [DOI] [PubMed] [Google Scholar]
  3. Brun G. M., Assairi L. M., Chapeville F. Immunological relationships between chick embryo polymerases. J Biol Chem. 1975 Sep 25;250(18):7320–7323. [PubMed] [Google Scholar]
  4. Chang L. M., Bollum F. J. Antigenic relationships in mammalian DNA polymerase. Science. 1972 Mar 10;175(4026):1116–1117. doi: 10.1126/science.175.4026.1116. [DOI] [PubMed] [Google Scholar]
  5. Chang L. M. Low molecular weight deoxyribonucleic acid polymerase from calf thymus chromatin. I. Preparation of homogeneous enzyme. J Biol Chem. 1973 Jun 10;248(11):3789–3795. [PubMed] [Google Scholar]
  6. Chen Y. C., Bohn E. W., Planck S. R., Wilson S. H. Mouse DNA polymerase alpha. Subunit structure and identification of a species with associated exonuclease. J Biol Chem. 1979 Nov 25;254(22):11678–11687. [PubMed] [Google Scholar]
  7. Elder J. H., Pickett R. A., 2nd, Hampton J., Lerner R. A. Radioiodination of proteins in single polyacrylamide gel slices. Tryptic peptide analysis of all the major members of complex multicomponent systems using microgram quantities of total protein. J Biol Chem. 1977 Sep 25;252(18):6510–6515. [PubMed] [Google Scholar]
  8. Holmer A. M., Hesslewood I. P., Johnston I. R. The occurrence of multiple activities in the high-molecular-weight DNA polymerase fraction of mammalian tissues. A preliminary study of some of their properties. Eur J Biochem. 1974 Apr 16;43(3):487–499. doi: 10.1111/j.1432-1033.1974.tb03436.x. [DOI] [PubMed] [Google Scholar]
  9. Kunkel T. A., Tcheng J. E., Meyer R. R. Purification and properties of DNA polymerase-beta from guinea pig liver. Biochim Biophys Acta. 1978 Sep 27;520(2):302–316. doi: 10.1016/0005-2787(78)90229-0. [DOI] [PubMed] [Google Scholar]
  10. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  11. Lin A., Collatz E., Wool I. G. Micro-scale two-dimensional polyacrylamide gell electrophoresis of ribosomal proteins. Mol Gen Genet. 1976 Feb 27;144(1):1–9. doi: 10.1007/BF00277296. [DOI] [PubMed] [Google Scholar]
  12. Mizutani S., Temin H. M. Lack of serological relationship among DNA polymerases of avian leukosis-sarcoma viruses, reticuloendotheliosis viruses, and chicken cells. J Virol. 1973 Sep;12(3):440–448. doi: 10.1128/jvi.12.3.440-448.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Mizutani S., Temin H. M. Specific serological relationships among partially purified DNA polymerases of avian leukosis-sarcoma viruses, reticuloendotheliosis viruses, and avian cells. J Virol. 1974 May;13(5):1020–1029. doi: 10.1128/jvi.13.5.1020-1029.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Spadari S., Muller R., Weissbach A. The dissimilitude of the low and high molecular weight deoxyribonucleic acid-dependent deoxyribonucleic acid polymerases of HeLa cells. J Biol Chem. 1974 May 10;249(9):2991–2992. [PubMed] [Google Scholar]
  15. Stalker D. M., Mosbaugh D. W., Meyer R. R. Novikoff hepatoma deoxyribonucleic acid polymerase. Purification and properties of a homogeneous beta polymerase. Biochemistry. 1976 Jul 13;15(14):3114–3121. doi: 10.1021/bi00659a027. [DOI] [PubMed] [Google Scholar]
  16. Tanabe K., Bohn E. W., Wilson S. H. Steady-state kinetics of mouse DNA polymerase beta. Biochemistry. 1979 Jul 24;18(15):3401–3406. doi: 10.1021/bi00582a029. [DOI] [PubMed] [Google Scholar]
  17. Wang T. S., Eichler D. C., Korn D. Effect of Mn2+ on the in vitro activity of human deoxyribonucleic acid polymerase beta. Biochemistry. 1977 Nov 1;16(22):4927–4934. doi: 10.1021/bi00641a029. [DOI] [PubMed] [Google Scholar]
  18. Wang T. S., Sedwick W. D., Korn D. Nuclear deoxyribonucleic acid polymerase. Purification and properties of the homogeneous enzyme from human KB cells. J Biol Chem. 1974 Feb 10;249(3):841–850. [PubMed] [Google Scholar]
  19. Yoshida S., Kondo T., Ando T. Multiple molecular species of cytoplasmic DNA polymerase from calf thymus. Biochim Biophys Acta. 1974 Jul 24;353(4):463–474. doi: 10.1016/0005-2787(74)90052-5. [DOI] [PubMed] [Google Scholar]

Articles from Nucleic Acids Research are provided here courtesy of Oxford University Press

RESOURCES