Skip to main content
PMC home page
Nucleic Acids Research logoLink to Nucleic Acids Research
. 1980 Jul 11;8(13):2859–2869. doi: 10.1093/nar/8.13.2859

The release of high mobility group protein H6 and protamine gene sequences upon selective DNase I degradation of trout testis chromatin.

B Levy-Wilson, L Kuehl, G H Dixon
PMCID: PMC324130  PMID: 6253894

Abstract

Limited digestion of trout testis nuclei with DNase I selectively degrades the protamine genes. Concomitant with the degradation of transcribed DNA sequences a series of chromosomal proteins are released; among these, the major species corresponds to the high mobility group protein H6. The amounts of H6 released from chromatin by limited DNase I action and that in the residual nuclear pellet have been determined. A very high proportion of H6 is associated with DNase I sensitive chromatin regions.

Full text

PDF
2863

Images in this article

2865Image
on p.2865

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Garel A., Axel R. Selective digestion of transcriptionally active ovalbumin genes from oviduct nuclei. Proc Natl Acad Sci U S A. 1976 Nov;73(11):3966–3970. doi: 10.1073/pnas.73.11.3966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Goldstein G., Scheid M., Hammerling U., Schlesinger D. H., Niall H. D., Boyse E. A. Isolation of a polypeptide that has lymphocyte-differentiating properties and is probably represented universally in living cells. Proc Natl Acad Sci U S A. 1975 Jan;72(1):11–15. doi: 10.1073/pnas.72.1.11. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Goodwin G. H., Johns E. W. Are the high mobility group non-histone chromosomal proteins associated with 'active' chromatin? Biochim Biophys Acta. 1978 Jun 22;519(1):279–284. doi: 10.1016/0005-2787(78)90081-3. [DOI] [PubMed] [Google Scholar]
  4. Hutcheon T., Dixon G. H., Levy-Wilson B. Transcriptionally active mononucleosomes from trout testis are heterogeneous in composition. J Biol Chem. 1980 Jan 25;255(2):681–685. [PubMed] [Google Scholar]
  5. Kornberg R. D. Structure of chromatin. Annu Rev Biochem. 1977;46:931–954. doi: 10.1146/annurev.bi.46.070177.004435. [DOI] [PubMed] [Google Scholar]
  6. Kuehl L., Lyness T., Dixon G. H., Levy-Wilson B. Distribution of high mobility group proteins among domains of trout testis chromatin differing in their susceptibility to micrococcal nuclease. J Biol Chem. 1980 Feb 10;255(3):1090–1095. [PubMed] [Google Scholar]
  7. Levy W B., Wong N. C., Dixon G. H. Selective association of the trout-specific H6 protein with chromatin regions susceptible to DNase I and DNase II: possible location of HMG-T in the spacer region between core nucleosomes. Proc Natl Acad Sci U S A. 1977 Jul;74(7):2810–2814. doi: 10.1073/pnas.74.7.2810. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Levy B. W., Connor W., Dixon G. H. A subset of trout testis nucleosomes enriched in transcribed DNA sequences contains high mobility group proteins as major structural components. J Biol Chem. 1979 Feb 10;254(3):609–620. [PubMed] [Google Scholar]
  9. Levy B., Dixon G. H. Diversity of sequences of polyadenylated cytoplasmic RNA from rainbow trout (Salmo gairdnerii) testis and liver. Biochemistry. 1977 Mar 8;16(5):958–964. doi: 10.1021/bi00624a023. [DOI] [PubMed] [Google Scholar]
  10. Levy W. B., Dixon G. H. A study of the localization of high mobility group proteins in chromatin. Can J Biochem. 1978 Jun;56(6):480–491. doi: 10.1139/o78-075. [DOI] [PubMed] [Google Scholar]
  11. Levy W. B., Dixon G. H. Reiteration frequency of the protamine genes in rainbow trout (Salmo gairdnerii). J Biol Chem. 1977 Nov 25;252(22):8062–8065. [PubMed] [Google Scholar]
  12. Vidali G., Boffa L. C., Allfrey V. G. Selective release of chromosomal proteins during limited DNAase 1 digestion of avian erythrocyte chromatin. Cell. 1977 Oct;12(2):409–415. doi: 10.1016/0092-8674(77)90117-9. [DOI] [PubMed] [Google Scholar]
  13. Walker J. M., Goodwin G. H., Johns E. W. The isolation and identification of ubiquitin from the high mobility group (HMG) non-histone protein fraction. FEBS Lett. 1978 Jun 15;90(2):327–330. doi: 10.1016/0014-5793(78)80397-4. [DOI] [PubMed] [Google Scholar]
  14. Watson D. C., Levy W. B., Dixon G. H. Free ubiquitin is a non-histone protein of trout testis chromatin. Nature. 1978 Nov 9;276(5684):196–198. doi: 10.1038/276196a0. [DOI] [PubMed] [Google Scholar]
  15. Watson D. C., Peters E. H., Dixon G. H. The purification, characterization and partial sequence determination of a trout testis non-histone protein, HMG-T. Eur J Biochem. 1977 Mar 15;74(1):53–60. doi: 10.1111/j.1432-1033.1977.tb11365.x. [DOI] [PubMed] [Google Scholar]
  16. Watson D. C., Wong N. C., Dixon G. H. The complete amino-acid sequence of a trout-testis non-histone protein, H6, localized in a subset of nucleosomes and its similarity to calf-thymus non-histone proteins HMG-14 and HMG-17. Eur J Biochem. 1979 Mar 15;95(1):193–202. doi: 10.1111/j.1432-1033.1979.tb12953.x. [DOI] [PubMed] [Google Scholar]
  17. Weintraub H., Groudine M. Chromosomal subunits in active genes have an altered conformation. Science. 1976 Sep 3;193(4256):848–856. doi: 10.1126/science.948749. [DOI] [PubMed] [Google Scholar]
  18. Weisbrod S., Weintraub H. Isolation of a subclass of nuclear proteins responsible for conferring a DNase I-sensitive structure on globin chromatin. Proc Natl Acad Sci U S A. 1979 Feb;76(2):630–634. doi: 10.1073/pnas.76.2.630. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Wong N. C., Poirier G. G., Dixon G. H. Adenosine diphosphoribosylation of certain basic chromosomal proteins in isolated trout testis nuclei. Eur J Biochem. 1977 Jul 1;77(1):11–21. doi: 10.1111/j.1432-1033.1977.tb11635.x. [DOI] [PubMed] [Google Scholar]

Articles from Nucleic Acids Research are provided here courtesy of Oxford University Press

RESOURCES