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. 1980 Aug 25;8(16):3611–3622. doi: 10.1093/nar/8.16.3611

Variability in the molecular organization of the 5S RNA genes among strains of Drosophila melanogaster.

N Junakovic
PMCID: PMC324179  PMID: 6159583

Abstract

The organization of the 5S RNA cluster has been analyzed in four strains of Drosophila melanogaster by the Southern technique. In some of the strains the 5S RNA cluster appears to be interrupted by an unrelated sequence. In other strains a continuous cluster is present.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Artavanis-Tsakonas S., Schedl P., Tschudi C., Pirrotta V., Steward R., Gehring W. J. The 5S genes of Drosophila melanogaster. Cell. 1977 Dec;12(4):1057–1067. doi: 10.1016/0092-8674(77)90169-6. [DOI] [PubMed] [Google Scholar]
  2. Denhardt D. T. A membrane-filter technique for the detection of complementary DNA. Biochem Biophys Res Commun. 1966 Jun 13;23(5):641–646. doi: 10.1016/0006-291x(66)90447-5. [DOI] [PubMed] [Google Scholar]
  3. Hershey N. D., Conrad S. E., Sodja A., Yen P. H., Cohen M., Jr, Davidson N., Iigen C., Carbon J. The sequence arrangement of Drosophila melanogaster 5s DNA cloned in recombinant plasmids. Cell. 1977 Jul;11(3):585–598. doi: 10.1016/0092-8674(77)90076-9. [DOI] [PubMed] [Google Scholar]
  4. Kim J. S., Davidson N. Electron microscope heteroduplex study of sequence relations of T2, T4, and T6 bacteriophage DNAs. Virology. 1974 Jan;57(1):93–111. doi: 10.1016/0042-6822(74)90111-1. [DOI] [PubMed] [Google Scholar]
  5. Laskey R. A., Mills A. D. Enhanced autoradiographic detection of 32P and 125I using intensifying screens and hypersensitized film. FEBS Lett. 1977 Oct 15;82(2):314–316. doi: 10.1016/0014-5793(77)80609-1. [DOI] [PubMed] [Google Scholar]
  6. Loening U. E. The determination of the molecular weight of ribonucleic acid by polyacrylamide-gel electrophresis. The effects of changes in conformation. Biochem J. 1969 Jun;113(1):131–138. doi: 10.1042/bj1130131. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Maniatis T., Jeffrey A., Kleid D. G. Nucleotide sequence of the rightward operator of phage lambda. Proc Natl Acad Sci U S A. 1975 Mar;72(3):1184–1188. doi: 10.1073/pnas.72.3.1184. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Procunier J. D., Dunn R. J. Genetic and molecular organization of the 5S locus and mutants in D. melanogaster. Cell. 1978 Nov;15(3):1087–1093. doi: 10.1016/0092-8674(78)90292-1. [DOI] [PubMed] [Google Scholar]
  9. Procunier J. D., Tartof K. D. Genetic analysis of the 5S RNA genes in Drosophila melanogaster. Genetics. 1975 Nov;81(3):515–523. doi: 10.1093/genetics/81.3.515. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Procunier J. D., Tartof K. D. Restriction map of 5S RNA genes of Drosophila melanogaster. Nature. 1976 Sep 16;263(5574):255–257. doi: 10.1038/263255a0. [DOI] [PubMed] [Google Scholar]
  11. Roberts R. J. Restriction endonucleases. CRC Crit Rev Biochem. 1976 Nov;4(2):123–164. doi: 10.3109/10409237609105456. [DOI] [PubMed] [Google Scholar]
  12. Rudkin G. T., Stollar B. D. High resolution detection of DNA-RNA hybrids in situ by indirect immunofluorescence. Nature. 1977 Feb 3;265(5593):472–473. doi: 10.1038/265472a0. [DOI] [PubMed] [Google Scholar]
  13. Southern E. M. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975 Nov 5;98(3):503–517. doi: 10.1016/s0022-2836(75)80083-0. [DOI] [PubMed] [Google Scholar]
  14. Tartof K. D., Perry R. P. The 5 s RNA genes of Drosophila melanogaster. J Mol Biol. 1970 Jul 28;51(2):171–183. doi: 10.1016/0022-2836(70)90135-x. [DOI] [PubMed] [Google Scholar]
  15. Wimber D. E., Steffensen D. M. Localization of 5S RNA genes on Drosophila chromosomes by RNA-DNA hybridization. Science. 1970 Nov 6;170(3958):639–641. doi: 10.1126/science.170.3958.639. [DOI] [PubMed] [Google Scholar]

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