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. 1980 Nov 11;8(21):4943–4954. doi: 10.1093/nar/8.21.4943

A comparison of DNA cleavage by the restriction enzymes SalPI and PstI.

J A Carter, K F Chater, C J Bruton, N L Brown
PMCID: PMC324271  PMID: 6255438

Abstract

Methods for obtaining highly active, exonuclease-free, stable preparations of the Streptomyces albus P restriction enzyme SalPI are described. SalPI and its isoschizomer PstI (from the taxonomically distant Providencia stuartii 164) both cleave their recognition sequence (5'-CTGCAG-3') to generate fragments terminating in tetranucleotide 3' extensions whose sequence is 5'-TGCA-3'. Bacteriophage R4G2 DNA, protected against SalPI cleavage by pregrowth on S. albus P, is also protected against PstI cleavage; and total DNA of both S. albus P and P. stuartii 164 is resistant to cleavage by both enzymes.

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Selected References

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