Skip to main content
PMC home page
Nucleic Acids Research logoLink to Nucleic Acids Research
. 1980 Nov 11;8(21):5071–5088. doi: 10.1093/nar/8.21.5071

Mapping of promoter sites utilized by T3 RNA polymerase on T3 DNA.

J N Bailey, W T McAllister
PMCID: PMC324280  PMID: 7443532

Abstract

Promoter locations for the T3 RNA polymerase on the physical map of T3 DNa have been determined. Through the use of conditions favoring the synthesis of RNA from the class II region, an agarose-formaldehyde gel system which improves the resolution of high molecular weight RNAs, and template DNA that was cut by one of several restriction endonucleases prior to transcription, seventeen promoter locations for the T3 RNA polymerase have been mapped. Ten promoters have been identified in the class II region and one promotor has been identified in the class II region and one promotor has been identified in the early (class I) region. The locations of previously mapped class III promoters and the internal termination signal for the T3 RNA polymerase have been mapped more precisely than in previous reports. The resulting transcription map demonstrates a striking similarity to the transcription map of bacteriophage T7.

Full text

PDF
5088

Images in this article

5078Image
on p.5078
5080Image
on p.5080
5081Image
on p.5081
5085Image
on p.5085

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bailey J. N., Dembinski D. R., McAllister W. T. Derivation of a restriction map of bacteriophage T3 DNA and comparison with the map of bacteriophage T7 DNA. J Virol. 1980 Jul;35(1):176–183. doi: 10.1128/jvi.35.1.176-183.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Beier H., Golomb M., Chamberlin M. Isolation of recombinants between T7 and T3 bacteriophages and their use in vitro transcriptional mapping. J Virol. 1977 Feb;21(2):753–765. doi: 10.1128/jvi.21.2.753-765.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Beier H., Hausmann R. Genetic map of bacteriophage T3. J Virol. 1973 Aug;12(2):417–419. doi: 10.1128/jvi.12.2.417-419.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Chakraborty P. R., Bandyopadhyay P., Huang H. H., Maitra U. Fidelity of in vitro transcription of T3 deoxyribonucleic acid by bacteriophage T3-induced ribonucleic acid polymerase and by Escherichia coli ribonucleic acid polymerase. J Biol Chem. 1974 Nov 10;249(21):6901–6909. [PubMed] [Google Scholar]
  5. Chakraborty P. R., Salvo R. A., Majumder H. K., Maitra U. Further characterization of bacteriophage T3-induced ribonucleic acid polymerase. Studies on the size of in vitro transcripts and interaction of T3 RNA polymerase with T3 DNA. J Biol Chem. 1977 Sep 25;252(18):6485–6493. [PubMed] [Google Scholar]
  6. Chakraborty P. R., Sarkar P., Huang H. H., Maitra U. Studies on T3-induced ribonucleic acid polymerase. 3. Purification and characterization of the T3-induced ribonucleic acid polymerase from bacteriophage T3-infected Escherichia coli cells. J Biol Chem. 1973 Oct 10;248(19):6637–6646. [PubMed] [Google Scholar]
  7. Chamberlin M., McGrath J., Waskell L. New RNA polymerase from Escherichia coli infected with bacteriophage T7. Nature. 1970 Oct 17;228(5268):227–231. doi: 10.1038/228227a0. [DOI] [PubMed] [Google Scholar]
  8. Davis R. W., Hyman R. W. A study in evolution: the DNA base sequence homology between coliphages T7 and T3. J Mol Biol. 1971 Dec 14;62(2):287–301. doi: 10.1016/0022-2836(71)90428-1. [DOI] [PubMed] [Google Scholar]
  9. Dunn J. J., Bautz F. A., Bautz E. K. Different template specificities of phage T3 and T7 RNA polymerases. Nat New Biol. 1971 Mar 17;230(11):94–96. doi: 10.1038/newbio230094a0. [DOI] [PubMed] [Google Scholar]
  10. Dunn J. J., McAllister W. T., Bautz E. K. In vitro transcription of T3 DNA by Escherichia coli and T3 polymerases. Virology. 1972 Apr;48(1):112–125. doi: 10.1016/0042-6822(72)90119-5. [DOI] [PubMed] [Google Scholar]
  11. Dunn J. J., McAllister W. T., Bautz E. K. Transcription in vitro of T3 DNA by Escherichia coli and T3 RNA polymerases. Analysis of the products in cell-free protein-synthesizing system. Eur J Biochem. 1972 Sep 25;29(3):500–508. doi: 10.1111/j.1432-1033.1972.tb02014.x. [DOI] [PubMed] [Google Scholar]
  12. Dunn J. J., Studier F. W. Effect of RNAase III, cleavage on translation of bacteriophage T7 messenger RNAs. J Mol Biol. 1975 Dec 15;99(3):487–499. doi: 10.1016/s0022-2836(75)80140-9. [DOI] [PubMed] [Google Scholar]
  13. Golomb M., Chamberlin M. J. T7- and T3-specific RNA polymerases: characterization and mapping of the in vitro transcripts read from T3 DNA. J Virol. 1977 Feb;21(2):743–752. doi: 10.1128/jvi.21.2.743-752.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Hausmann R. Bacteriophage T7 genetics. Curr Top Microbiol Immunol. 1976;75:77–110. doi: 10.1007/978-3-642-66530-1_3. [DOI] [PubMed] [Google Scholar]
  15. Hayward G. S. Gel electrophoretic separation of the complementary strands of bacteriophage DNA. Virology. 1972 Jul;49(1):342–344. doi: 10.1016/s0042-6822(72)80042-4. [DOI] [PubMed] [Google Scholar]
  16. Kassavetis G. A., Chamberlin M. J. Mapping of class II promoter sites utilized in vitro by T7-specific RNA polymerase on bacteriophage T7 DNA. J Virol. 1979 Jan;29(1):196–208. doi: 10.1128/jvi.29.1.196-208.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Lehrach H., Diamond D., Wozney J. M., Boedtker H. RNA molecular weight determinations by gel electrophoresis under denaturing conditions, a critical reexamination. Biochemistry. 1977 Oct 18;16(21):4743–4751. doi: 10.1021/bi00640a033. [DOI] [PubMed] [Google Scholar]
  18. Maitra U. Induction of a new RNA polymerase in Escherichia coli infected with bacteriophage T3. Biochem Biophys Res Commun. 1971 Apr 16;43(2):443–450. doi: 10.1016/0006-291x(71)90773-x. [DOI] [PubMed] [Google Scholar]
  19. McAllister W. T., Barrett C. L. Hybridization mapping of restriction fragments from the early region of bacteriophage T7 DNA. Virology. 1977 Oct 15;82(2):275–287. doi: 10.1016/0042-6822(77)90003-4. [DOI] [PubMed] [Google Scholar]
  20. McAllister W. T., Küpper H., Bautz E. K. Kinetics of transcription by the bacteriophage-T3 RNA polymerase in vitro. Eur J Biochem. 1973 May 2;34(3):489–501. doi: 10.1111/j.1432-1033.1973.tb02785.x. [DOI] [PubMed] [Google Scholar]
  21. McAllister W. T., Wu H. L. Regulation of transcription of the late genes of bacteriophage T7. Proc Natl Acad Sci U S A. 1978 Feb;75(2):804–808. doi: 10.1073/pnas.75.2.804. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. McCorquodale D. J. The T-odd bacteriophages. CRC Crit Rev Microbiol. 1975 Dec;4(2):101–159. doi: 10.3109/10408417509111574. [DOI] [PubMed] [Google Scholar]
  23. McDonell M. W., Simon M. N., Studier F. W. Analysis of restriction fragments of T7 DNA and determination of molecular weights by electrophoresis in neutral and alkaline gels. J Mol Biol. 1977 Feb 15;110(1):119–146. doi: 10.1016/s0022-2836(77)80102-2. [DOI] [PubMed] [Google Scholar]
  24. Musielski H., Mann W., Michel S. Transcription by bacteriophage T3-induced RNA polymerase in the presence of KCl and dimethylsulfoxide. Z Allg Mikrobiol. 1979;19(1):61–63. doi: 10.1002/jobm.3630190110. [DOI] [PubMed] [Google Scholar]
  25. Sharp P. A., Sugden B., Sambrook J. Detection of two restriction endonuclease activities in Haemophilus parainfluenzae using analytical agarose--ethidium bromide electrophoresis. Biochemistry. 1973 Jul 31;12(16):3055–3063. doi: 10.1021/bi00740a018. [DOI] [PubMed] [Google Scholar]
  26. Sternbach H., Engelhardt R., Lezius A. G. Rapid isolation of highly active RNA polymerase from Escherichia coli and its subunits by matrix-bound heparin. Eur J Biochem. 1975 Dec 1;60(1):51–55. doi: 10.1111/j.1432-1033.1975.tb20974.x. [DOI] [PubMed] [Google Scholar]
  27. Studier F. W. Analysis of bacteriophage T7 early RNAs and proteins on slab gels. J Mol Biol. 1973 Sep 15;79(2):237–248. doi: 10.1016/0022-2836(73)90003-x. [DOI] [PubMed] [Google Scholar]
  28. Studier F. W. Bacteriophage T7. Science. 1972 Apr 28;176(4033):367–376. doi: 10.1126/science.176.4033.367. [DOI] [PubMed] [Google Scholar]
  29. Studier F. W. Relationships among different strains of T7 and among T7-related bacteriophages. Virology. 1979 May;95(1):70–84. doi: 10.1016/0042-6822(79)90402-1. [DOI] [PubMed] [Google Scholar]

Articles from Nucleic Acids Research are provided here courtesy of Oxford University Press

RESOURCES