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. 1980 Nov 25;8(22):5267–5274. doi: 10.1093/nar/8.22.5267

DNA sequence of the araC regulatory gene from Escherichia coli B/r.

C G Miyada, A H Horwitz, L G Cass, J Timko, G Wilcox
PMCID: PMC324300  PMID: 7008027

Abstract

The DNA sequence of the araC regulatory gene from Escherichia coli B/r has been determined by the base-specific chemical cleavage reactions of Maxam and Gilbert. An open reading frame is found which codes for a protein of 292 amino acids. A nonsense mutation, araC5, is shown to result from a G to A transition at nucleotide 429 converting the tryptophan codon TGG to the amber codon TAG. A deletion which does not recombine with any known point mutation in araC, delta(araCO)719, removes all but the last 22 codons of the gene.

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Selected References

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