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. 1980 Dec 11;8(23):5725–5737. doi: 10.1093/nar/8.23.5725

Yeast histone mRNA is polyadenylated.

K Fahrner, J Yarger, L Hereford
PMCID: PMC324337  PMID: 7008028

Abstract

Histone mRNA from S. cerevisiae has been identified and partially purified. The RNA is quantitatively retained on oligo (dT) cellulose or poly(U) sepharose as assayed by in vitro translation or hybridization of radiolabelled cloned yeast histone sequences to RNA immobilized on DBM paper. Retention of yeast histone mRNA on either of these chromatographic systems is most likely the result of polyadenylation since, when primed with oligo (dT), the RNA is an extremely good template for reverse transcriptase, as determined by hybrid arrest translation or by hybridization to D. melanogaster histone DNA sequences.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Alwine J. C., Kemp D. J., Stark G. R. Method for detection of specific RNAs in agarose gels by transfer to diazobenzyloxymethyl-paper and hybridization with DNA probes. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5350–5354. doi: 10.1073/pnas.74.12.5350. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bonner W. M., Laskey R. A. A film detection method for tritium-labelled proteins and nucleic acids in polyacrylamide gels. Eur J Biochem. 1974 Jul 1;46(1):83–88. doi: 10.1111/j.1432-1033.1974.tb03599.x. [DOI] [PubMed] [Google Scholar]
  3. Borun T. W., Ajiro K., Zweidler A., Dolby T. W., Stephens R. E. Studies of human histone messenger RNA. II. The resolution of fractions containing individual human histone messenger RNA species. J Biol Chem. 1977 Jan 10;252(1):173–180. [PubMed] [Google Scholar]
  4. Cabada M. O., Darnbrough C., Ford P. J., Turner P. C. Differential accumulation of two size classes of poly(A) associated with messenger RNA during oogenesis in Xenopus laevis. Dev Biol. 1977 Jun;57(2):427–439. doi: 10.1016/0012-1606(77)90227-5. [DOI] [PubMed] [Google Scholar]
  5. Clewell D. B., Helinski D. R. Supercoiled circular DNA-protein complex in Escherichia coli: purification and induced conversion to an opern circular DNA form. Proc Natl Acad Sci U S A. 1969 Apr;62(4):1159–1166. doi: 10.1073/pnas.62.4.1159. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Elgin S. C., Weintraub H. Chromosomal proteins and chromatin structure. Annu Rev Biochem. 1975;44:725–774. doi: 10.1146/annurev.bi.44.070175.003453. [DOI] [PubMed] [Google Scholar]
  7. Elliott S. G., McLaughlin C. S. Rate of macromolecular synthesis through the cell cycle of the yeast Saccharomyces cerevisiae. Proc Natl Acad Sci U S A. 1978 Sep;75(9):4384–4388. doi: 10.1073/pnas.75.9.4384. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Gabrielli F., Baglioni C. Maternal messenger RNA and histone synthesis in embryos of the surf clam Spisula solidissima. Dev Biol. 1975 Apr;43(2):254–263. doi: 10.1016/0012-1606(75)90025-1. [DOI] [PubMed] [Google Scholar]
  9. Hagen F. S. Large-scale preparative polyacrylamide gel electrophoresis of ribonucleic acid. Anal Biochem. 1979 Mar;93(2):299–305. doi: 10.1016/s0003-2697(79)80155-4. [DOI] [PubMed] [Google Scholar]
  10. Hereford L. M., Rosbash M. Number and distribution of polyadenylated RNA sequences in yeast. Cell. 1977 Mar;10(3):453–462. doi: 10.1016/0092-8674(77)90032-0. [DOI] [PubMed] [Google Scholar]
  11. Hereford L. M., Rosbash M. Regulation of a set of abundant mRNA sequences. Cell. 1977 Mar;10(3):463–467. doi: 10.1016/0092-8674(77)90033-2. [DOI] [PubMed] [Google Scholar]
  12. Hereford L., Fahrner K., Woolford J., Jr, Rosbash M., Kaback D. B. Isolation of yeast histone genes H2A and H2B. Cell. 1979 Dec;18(4):1261–1271. doi: 10.1016/0092-8674(79)90237-x. [DOI] [PubMed] [Google Scholar]
  13. Kedes L. H. Histone genes and histone messengers. Annu Rev Biochem. 1979;48:837–870. doi: 10.1146/annurev.bi.48.070179.004201. [DOI] [PubMed] [Google Scholar]
  14. Lifton R. P., Goldberg M. L., Karp R. W., Hogness D. S. The organization of the histone genes in Drosophila melanogaster: functional and evolutionary implications. Cold Spring Harb Symp Quant Biol. 1978;42(Pt 2):1047–1051. doi: 10.1101/sqb.1978.042.01.105. [DOI] [PubMed] [Google Scholar]
  15. Maniatis T., Jeffrey A., Kleid D. G. Nucleotide sequence of the rightward operator of phage lambda. Proc Natl Acad Sci U S A. 1975 Mar;72(3):1184–1188. doi: 10.1073/pnas.72.3.1184. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Mardian J. K., Isenberg I. Yeast inner histones and the evolutionary conservation of histone-histone interactions. Biochemistry. 1978 Sep 5;17(18):3825–3833. doi: 10.1021/bi00611a023. [DOI] [PubMed] [Google Scholar]
  17. Roberts B. E., Paterson B. M. Efficient translation of tobacco mosaic virus RNA and rabbit globin 9S RNA in a cell-free system from commercial wheat germ. Proc Natl Acad Sci U S A. 1973 Aug;70(8):2330–2334. doi: 10.1073/pnas.70.8.2330. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Rosbash M. Polyadenylic acid-containing RNA in Xenopus laevis oocytes. J Mol Biol. 1974 May 5;85(1):87–101. doi: 10.1016/0022-2836(74)90131-4. [DOI] [PubMed] [Google Scholar]
  19. Ruderman J. V., Pardue M. L. A portion of all major classes of histone messenger RNA in amphibian oocytes is polyadenylated. J Biol Chem. 1978 Mar 25;253(6):2018–2025. [PubMed] [Google Scholar]
  20. Ruderman J. V., Pardue M. L. Cell-free translation analysis of messenger RNA in echinoderm and amphibian early development. Dev Biol. 1977 Oct 1;60(1):48–68. doi: 10.1016/0012-1606(77)90109-9. [DOI] [PubMed] [Google Scholar]
  21. Ruderman J. V., Woodland H. R., Sturgess E. A. Modulations of histone messenger RNA during the early development of Xenopus laevis. Dev Biol. 1979 Jul;71(1):71–82. doi: 10.1016/0012-1606(79)90083-6. [DOI] [PubMed] [Google Scholar]
  22. Southern E. M. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975 Nov 5;98(3):503–517. doi: 10.1016/s0022-2836(75)80083-0. [DOI] [PubMed] [Google Scholar]
  23. Spiker S., Key J. L., Wakim B. Identification and fractionation of plant histones. Arch Biochem Biophys. 1976 Oct;176(2):510–518. doi: 10.1016/0003-9861(76)90194-6. [DOI] [PubMed] [Google Scholar]

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