Skip to main content
PMC home page
Nucleic Acids Research logoLink to Nucleic Acids Research
. 1983 Feb 11;11(3):605–617. doi: 10.1093/nar/11.3.605

Chemical reactivity of E. coli 5S RNA in situ in the 50S ribosomal subunit.

M Silberklang, U L RajBhandary, A Lück, V A Erdmann
PMCID: PMC325740  PMID: 6340064

Abstract

E. coli 50S ribosomal subunits were reacted with monoperphthalic acid under conditions in which non-base paired adenines are modified to their 1-N-oxides. 5S RNA was isolated from such chemically reacted subunits and the two modified adenines were identified as A73 and A99. The modified 5S RNA, when used in reconstitution of 50S subunits, yielded particles with reduced biological activity (50%). The results are discussed with respect to a recently proposed three-dimensional structure for 5S RNA, the interaction of the RNA with proteins E-L5, E-L18 and E-L25 and previously proposed interactions of 5S RNA with tRNA, 16S and 23S ribosomal RNAs.

Full text

PDF
617

Images in this article

610Image
on p.610
612Image
on p.612

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Azad A. A. Intermolecular base-paired interaction between complementary sequences present near the 3' ends of 5S rRNA and 18S (16S) rRNA might be involved in the reversible association of ribosomal subunits. Nucleic Acids Res. 1979 Dec 11;7(7):1913–1929. doi: 10.1093/nar/7.7.1913. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Brownlee G. G., Sanger F., Barrell B. G. The sequence of 5 s ribosomal ribonucleic acid. J Mol Biol. 1968 Jun 28;34(3):379–412. doi: 10.1016/0022-2836(68)90168-x. [DOI] [PubMed] [Google Scholar]
  3. Cramer F., Erdmann V. A. Amount of adenine and uracil base pairs in E. coli 23S, 16S and 5S ribosomal RNA. Nature. 1968 Apr 6;218(5136):92–93. doi: 10.1038/218092a0. [DOI] [PubMed] [Google Scholar]
  4. Cronenberger J. H., Erdmann V. A. Stimulation of polypeptide polymerization by blocking of free sulphydryl groups in Escherichia coli ribosomal proteins. J Mol Biol. 1975 Jun 15;95(1):125–137. doi: 10.1016/0022-2836(75)90340-x. [DOI] [PubMed] [Google Scholar]
  5. Digweed M., Kumagai I., Pieler T., Erdmann V. A. The effect of a cytidine-to-uridine transition on the stability of Escherichia coli A19 5-S RNA. Eur J Biochem. 1982 Oct;127(3):531–537. doi: 10.1111/j.1432-1033.1982.tb06904.x. [DOI] [PubMed] [Google Scholar]
  6. Erdmann V. A. Collection of published 5S and 5.8S RNA sequences and their precursors. Nucleic Acids Res. 1981 Jan 10;9(1):r25–r42. doi: 10.1093/nar/9.1.213-a. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Erdmann V. A., Doberer H. G. Structure and function of 5S RNA: the role of the 3' terminus in 5S RNA function. Mol Gen Genet. 1972;114(2):89–94. doi: 10.1007/BF00332779. [DOI] [PubMed] [Google Scholar]
  8. Erdmann V. A., Sprinzl M., Pongs O. The involvement of 5S RNA in the binding of tRNA to ribosomes. Biochem Biophys Res Commun. 1973 Oct 1;54(3):942–948. doi: 10.1016/0006-291x(73)90785-7. [DOI] [PubMed] [Google Scholar]
  9. Erdmann V. A. Structure and function of 5S and 5.8 S RNA. Prog Nucleic Acid Res Mol Biol. 1976;18:45–90. [PubMed] [Google Scholar]
  10. Farber N. M., Cantor C. R. Accessibility and structure of ribosomal RNA monitored by slow tritium exchange of ribosomes. J Mol Biol. 1981 Feb 25;146(2):241–257. doi: 10.1016/0022-2836(81)90434-4. [DOI] [PubMed] [Google Scholar]
  11. Forget B. G., Weissman S. M. Nucleotide sequence of KB cell 5S RNA. Science. 1967 Dec 29;158(3809):1695–1699. doi: 10.1126/science.158.3809.1695. [DOI] [PubMed] [Google Scholar]
  12. Gillum A. M., Urquhart N., Smith M., RajBhandary U. L. Nucleotide sequence of salmon testes and salmon liver cytoplasmic initiator tRNA. Cell. 1975 Nov;6(3):395–405. doi: 10.1016/0092-8674(75)90189-0. [DOI] [PubMed] [Google Scholar]
  13. Glotz C., Zwieb C., Brimacombe R., Edwards K., Kössel H. Secondary structure of the large subunit ribosomal RNA from Escherichia coli, Zea mays chloroplast, and human and mouse mitochondrial ribosomes. Nucleic Acids Res. 1981 Jul 24;9(14):3287–3306. doi: 10.1093/nar/9.14.3287. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Herr W., Noller H. F. A fragment of 23S RNA containing a nucleotide sequence complementary to a region of 5S RNA. FEBS Lett. 1975 May 1;53(2):248–252. doi: 10.1016/0014-5793(75)80030-5. [DOI] [PubMed] [Google Scholar]
  15. Horne J. R., Erdmann V. A. Isolation and characterization of 5S RNA-protein complexes from Bacillus stearothermophilus and Escherichia coli ribosomes. Mol Gen Genet. 1972;119(4):337–344. doi: 10.1007/BF00272091. [DOI] [PubMed] [Google Scholar]
  16. Nierhaus K. H., Dohme F. Total reconstitution of functionally active 50S ribosomal subunits from Escherichia coli. Proc Natl Acad Sci U S A. 1974 Dec;71(12):4713–4717. doi: 10.1073/pnas.71.12.4713. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Nomura M., Erdmann V. A. Reconstitution of 50S ribosomal subunits from dissociated molecular components. Nature. 1970 Nov 21;228(5273):744–748. doi: 10.1038/228744a0. [DOI] [PubMed] [Google Scholar]
  18. Ofengand J., Henes C. The function of pseudouridylic acid in transfer ribonucleic acid. II. Inhibition of amino acyl transfer ribonucleic acid-ribosome complex formation by ribothymidylyl-pseudouridylyl-cytidylyl-guanosine 3'-phosphate. J Biol Chem. 1969 Nov 25;244(22):6241–6253. [PubMed] [Google Scholar]
  19. Pace B., Matthews E. A., Johnson K. D., Cantor C. R., Pace N. R. Conserved 5S rRNA complement to tRNA is not required for protein synthesis. Proc Natl Acad Sci U S A. 1982 Jan;79(1):36–40. doi: 10.1073/pnas.79.1.36. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Pieler T., Erdmann V. A. Three-dimensional structural model of eubacterial 5S RNA that has functional implications. Proc Natl Acad Sci U S A. 1982 Aug;79(15):4599–4603. doi: 10.1073/pnas.79.15.4599. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Raué H. A., Lorenz S., Erdmann V. A., Planta R. J. Reconstitution of biologically active 50S ribosomal subunits with artificial 5S RNA molecules carrying disturbances in the base pairing within the molecular stalk. Nucleic Acids Res. 1981 Mar 11;9(5):1263–1269. doi: 10.1093/nar/9.5.1263. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Richter D., Erdmann V. A., Sprinzl M. A new transfer RNA fragment reaction: Tp psi pCpGp bound to a ribosome-messenger RNA complex induces the synthesis of guanosine tetra- and pentaphosphates. Proc Natl Acad Sci U S A. 1974 Aug;71(8):3226–3229. doi: 10.1073/pnas.71.8.3226. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Sanger F., Brownlee G. G., Barrell B. G. A two-dimensional fractionation procedure for radioactive nucleotides. J Mol Biol. 1965 Sep;13(2):373–398. doi: 10.1016/s0022-2836(65)80104-8. [DOI] [PubMed] [Google Scholar]
  24. Shimizu N., Hayashi H., Miura K. I. Functional sites of transfer RNA for the binding to messenger RNA-ribosome complex. J Biochem. 1970 Mar;67(3):373–387. doi: 10.1093/oxfordjournals.jbchem.a129261. [DOI] [PubMed] [Google Scholar]
  25. Silberklang M., Prochiantz A., Haenni A. L., Rajbhandary U. L. Studies on the sequence of the 3'-terminal region of turnip-yellow-mosaic-virus RNA. Eur J Biochem. 1977 Feb;72(3):465–478. doi: 10.1111/j.1432-1033.1977.tb11270.x. [DOI] [PubMed] [Google Scholar]
  26. Simsek M., Petrissant G., Rajbhandary U. L. Replacement of the sequence G-T-phi-C-G(A)- by G-A-U-C-G- in initiator transfer RNA of rabbit-liver cytoplasm. Proc Natl Acad Sci U S A. 1973 Sep;70(9):2600–2604. doi: 10.1073/pnas.70.9.2600. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Sprinzl M., Wagner T., Lorenz S., Erdmann V. A. Regions of tRNA important for binding to the ribosomal A and P sites. Biochemistry. 1976 Jul 13;15(14):3031–3039. doi: 10.1021/bi00659a015. [DOI] [PubMed] [Google Scholar]
  28. Uhlenbeck O. C., Baller J., Doty P. Complementary oligonucleotide binding to the anticodon loop of fMet-transfer RNA. Nature. 1970 Feb 7;225(5232):508–510. doi: 10.1038/225508a0. [DOI] [PubMed] [Google Scholar]
  29. Wrede P., Erdmann V. A. Activities of B. stearothermophilus 50 S ribosomes reconstituted with prokaryotic and eukaryotic 5 S RNA. FEBS Lett. 1973 Jul 15;33(3):315–319. doi: 10.1016/0014-5793(73)80219-4. [DOI] [PubMed] [Google Scholar]
  30. Wrede P., Pongs O., Erdmann V. A. Binding oligonucleotides to Escherichia coli and Bacillus stearothermophilus 5 S RNA. J Mol Biol. 1978 Mar 25;120(1):83–96. doi: 10.1016/0022-2836(78)90296-6. [DOI] [PubMed] [Google Scholar]
  31. Zimmermann J., Erdmann V. A. Identification of Escherichia coli and Bacillus stearothermophilus ribosomal protein binding sites on Escherichia coli 5S RNA. Mol Gen Genet. 1978 Apr 17;160(3):247–257. doi: 10.1007/BF00332968. [DOI] [PubMed] [Google Scholar]

Articles from Nucleic Acids Research are provided here courtesy of Oxford University Press

RESOURCES