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. 1983 May 25;11(10):3019–3025. doi: 10.1093/nar/11.10.3019

Complete nucleotide sequence of alfalfa mosaic virus RNA 2.

B J Cornelissen, F T Brederode, G H Veeneman, J H van Boom, J F Bol
PMCID: PMC325944  PMID: 6304618

Abstract

Double-stranded cDNA of in vitro polyadenylated alfalfa mosaic virus (AlMV) RNA 2 has been cloned and sequenced. The use of an oligodeoxyribonucleotide corresponding to the known sequence of the 5'-end of RNA 2 to prime second-strand DNA synthesis, enabled us to construct the complete primary structure of AlMV RNA 2. The sequence of 2,593 nucleotides contains a long open reading frame for a protein of Mr 89,753 starting at the first AUG codon from the 5'-end. This coding region is flanked by a 5'-terminal sequence of 54 nucleotides and a 3'-noncoding region of 166 nucleotides which includes the sequence of 145 nucleotides the three genomic RNAs of AlMV have in common.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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