Skip to main content
PMC home page
Nucleic Acids Research logoLink to Nucleic Acids Research
. 1983 Jun 11;11(11):3767–3778. doi: 10.1093/nar/11.11.3767

Molecular cloning and nucleotide sequence of the 30K and the coat protein cistron of TMV (tomato strain) genome.

N Takamatsu, T Ohno, T Meshi, Y Okada
PMCID: PMC326001  PMID: 6304642

Abstract

The cDNA copies of tobacco mosaic virus (TMV)-tomato strain (L) genome were cloned by the method of Okayama and Berg (Mol. Cell. Biol. 2, 161-170. (1982)) and the sequence of 1,614 nucleotides at the 3' end was determined. The sequence encompasses the 30K and the coat protein cistron which are located in residues 685-1, 479 and 203-682 from the 3' end of the genome respectively. The close relationship between the tomato and the common strain was shown on the level of the nucleotide sequence. Highly homologous regions are found in the 3' non-coding region, the assembly origin and the 5' flanking region of the 30K protein cistron. The comparison of the deduced amino acid sequence between the tomato and the common strain shows that the 30K protein is composed of the conserved N-terminal four-fifth and the highly divergent region near the C-terminus.

Full text

PDF
3769

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Beachy R. N., Zaitlin M., Bruening G., Israel H. W. A genetic map for the cowpea strain on TMV. Virology. 1976 Sep;73(2):498–507. doi: 10.1016/0042-6822(76)90411-6. [DOI] [PubMed] [Google Scholar]
  2. Bernard O., Gough N. M. Nucleotide sequence of immunoglobulin heavy chain joining segments between translocated VH and mu constant regions genes. Proc Natl Acad Sci U S A. 1980 Jun;77(6):3630–3634. doi: 10.1073/pnas.77.6.3630. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Fukuda M., Meshi T., Okada Y., Otsuki Y., Takebe I. Correlation between particle multiplicity and location on virion RNA of the assembly initiation site for viruses of the tobacco mosaic virus group. Proc Natl Acad Sci U S A. 1981 Jul;78(7):4231–4235. doi: 10.1073/pnas.78.7.4231. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Gibbs A. How ancient are the tobamoviruses? Intervirology. 1980;14(2):101–108. doi: 10.1159/000149169. [DOI] [PubMed] [Google Scholar]
  5. Goelet P., Lomonossoff G. P., Butler P. J., Akam M. E., Gait M. J., Karn J. Nucleotide sequence of tobacco mosaic virus RNA. Proc Natl Acad Sci U S A. 1982 Oct;79(19):5818–5822. doi: 10.1073/pnas.79.19.5818. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Grunstein M., Hogness D. S. Colony hybridization: a method for the isolation of cloned DNAs that contain a specific gene. Proc Natl Acad Sci U S A. 1975 Oct;72(10):3961–3965. doi: 10.1073/pnas.72.10.3961. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Guilley H., Jonard G., Kukla B., Richards K. E. Sequence of 1000 nucleotides at the 3' end of tobacco mosaic virus RNA. Nucleic Acids Res. 1979 Apr;6(4):1287–1308. doi: 10.1093/nar/6.4.1287. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Hirth L., Richards K. E. Tobacco mosaic virus: model for structure and function of a simple virus. Adv Virus Res. 1981;26:145–199. doi: 10.1016/s0065-3527(08)60423-6. [DOI] [PubMed] [Google Scholar]
  9. Hunter T. R., Hunt T., Knowland J., Zimmern D. Messenger RNA for the coat protein of tobacco mosaic virus. Nature. 1976 Apr 29;260(5554):759–764. doi: 10.1038/260759a0. [DOI] [PubMed] [Google Scholar]
  10. Jonard G., Richards K. E., Guilley H., Hirth L. Sequence from the assembly nucleation region of TMV RNA. Cell. 1977 Jul;11(3):483–493. doi: 10.1016/0092-8674(77)90066-6. [DOI] [PubMed] [Google Scholar]
  11. Katz L., Kingsbury D. T., Helinski D. R. Stimulation by cyclic adenosine monophosphate of plasmid deoxyribonucleic acid replication and catabolite repression of the plasmid deoxyribonucleic acid-protein relaxation complex. J Bacteriol. 1973 May;114(2):577–591. doi: 10.1128/jb.114.2.577-591.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Maxam A. M., Gilbert W. Sequencing end-labeled DNA with base-specific chemical cleavages. Methods Enzymol. 1980;65(1):499–560. doi: 10.1016/s0076-6879(80)65059-9. [DOI] [PubMed] [Google Scholar]
  13. Meshi T., Ohno T., Iba H., Okada Y. Nucleotide sequence of a cloned cDNA copy of TMV (cowpea strain) RNA, including the assembly origin, the coat protein cistron, and the 3' non-coding region. Mol Gen Genet. 1981;184(1):20–25. doi: 10.1007/BF00271189. [DOI] [PubMed] [Google Scholar]
  14. Meshi T., Ohno T., Okada Y. Nucleotide sequence and its character of cistron coding for the 30 K protein of tobacco mosaic virus (OM strain). J Biochem. 1982 Apr;91(4):1441–1444. doi: 10.1093/oxfordjournals.jbchem.a133833. [DOI] [PubMed] [Google Scholar]
  15. Meshi T., Ohno T., Okada Y. Nucleotide sequence of the 30K protein cistron of cowpea strain of tobacco mosaic virus. Nucleic Acids Res. 1982 Oct 11;10(19):6111–6117. doi: 10.1093/nar/10.19.6111. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Oda K., Kato H., Saito I., Sugano S., Maruyama K., Masuda M., Shiroki K., Shimojo H. Expression of adenovirus type 12 E1A gene in monkey cells, using a simian virus 40 vector. J Virol. 1983 Jan;45(1):408–419. doi: 10.1128/jvi.45.1.408-419.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Okayama H., Berg P. High-efficiency cloning of full-length cDNA. Mol Cell Biol. 1982 Feb;2(2):161–170. doi: 10.1128/mcb.2.2.161. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Peattie D. A. Direct chemical method for sequencing RNA. Proc Natl Acad Sci U S A. 1979 Apr;76(4):1760–1764. doi: 10.1073/pnas.76.4.1760. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Sippel A. E. Purification and characterization of adenosine triphosphate: ribonucleic acid adenyltransferase from Escherichia coli. Eur J Biochem. 1973 Aug 1;37(1):31–40. doi: 10.1111/j.1432-1033.1973.tb02953.x. [DOI] [PubMed] [Google Scholar]
  20. Tinoco I., Jr, Borer P. N., Dengler B., Levin M. D., Uhlenbeck O. C., Crothers D. M., Bralla J. Improved estimation of secondary structure in ribonucleic acids. Nat New Biol. 1973 Nov 14;246(150):40–41. doi: 10.1038/newbio246040a0. [DOI] [PubMed] [Google Scholar]
  21. WITTMANN-LIEBOLD B., WITTMANN H. G. DIE PRIMAERE PROTEINSTRUKTUR VON STAEMMEN DES TABAKMOSAIKVIRUS. AMINOSAEURESEQUENZEN DES PROTEINS DES TABAKMOSAIKVIRUSSTAMMES DAHLEMENSE. III. DISKUSSION DER ERGEBNISSE. Z Vererbungsl. 1963 Dec 30;94:427–435. [PubMed] [Google Scholar]
  22. Zimmern D. The nucleotide sequence at the origin for assembly on tobacco mosaic virus RNA. Cell. 1977 Jul;11(3):463–482. doi: 10.1016/0092-8674(77)90065-4. [DOI] [PubMed] [Google Scholar]

Articles from Nucleic Acids Research are provided here courtesy of Oxford University Press

RESOURCES