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. 1983 Jul 11;11(13):4541–4552. doi: 10.1093/nar/11.13.4541

Carp preproinsulin cDNA sequence and evolution of insulin genes.

V Hahn, J Winkler, T A Rapoport, D H Liebscher, C Coutelle, S Rosenthal
PMCID: PMC326063  PMID: 6306593

Abstract

The nucleic acid sequence of the preproinsulin cDNA of carp (Cyprinus carpio), cloned in the PstI-site of pBR322 (1), has been determined. The sequenced insert of 439 bp includes the complete coding information for carp preproinsulin (108 amino acids), 10 nucleotides of the 5'-and 105 nucleotides of the 3'-nontranslated regions. The nucleotide sequence confirms the previously established amino acid sequence of carp insulin (2) and determines those of the signal (21 aa 1) and C-peptide (35 aa 1). The observed shortness of the signal peptide of carp preproinsulin and the N-terminal addition of 2 amino acids to the carp insulin B-chain suggest that the cleavage site of the signal peptidase has moved. Calculations based on the comparison of known preproinsulin cDNA sequences showed that the evolutionary distance between fresh water and salt water teleostians is not smaller than between man and chicken.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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