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. 1983 Jul 25;11(14):4835–4852. doi: 10.1093/nar/11.14.4835

A repetitive and apparently transposable DNA sequence in Dictyostelium discoideum associated with developmentally regulated RNAs.

S Chung, C Zuker, H F Lodish
PMCID: PMC326089  PMID: 6308562

Abstract

Dictyostelium discoideum AX3 genomic DNA contains about 40 copies of a 4.5 Kb sequence. Each has the same restriction map, suggesting that they are all very similar. The sequences are scattered throughout the genome, and each of the six representative copies we cloned have different flanking sequences. Partial fragments of the 4.5 Kb sequence also are scattered throughout the genome. The DNA sequences flanking the 4.5 Kb repetitive sequences are different in different strains of Dictyostelium suggesting that the 4.5 Kb sequence is a transposable element. Each sub-region of this 4.5 Kb sequence is associated with a large number of mRNAs, all of which are developmentally regulated, but whose function is not known.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Benton W. D., Davis R. W. Screening lambdagt recombinant clones by hybridization to single plaques in situ. Science. 1977 Apr 8;196(4286):180–182. doi: 10.1126/science.322279. [DOI] [PubMed] [Google Scholar]
  2. Blumberg D. D., Lodish H. F. Complexity of nuclear and polysomal RNAs in growing Dictyostelium discoideum cells. Dev Biol. 1980 Aug;78(2):268–284. doi: 10.1016/0012-1606(80)90336-x. [DOI] [PubMed] [Google Scholar]
  3. Calos M. P., Miller J. H. Transposable elements. Cell. 1980 Jul;20(3):579–595. doi: 10.1016/0092-8674(80)90305-0. [DOI] [PubMed] [Google Scholar]
  4. Cameron J. R., Loh E. Y., Davis R. W. Evidence for transposition of dispersed repetitive DNA families in yeast. Cell. 1979 Apr;16(4):739–751. doi: 10.1016/0092-8674(79)90090-4. [DOI] [PubMed] [Google Scholar]
  5. Chung S., Landfear S. M., Blumberg D. D., Cohen N. S., Lodish H. F. Synthesis and stability of developmentally regulated dictyostelium mRNAs are affected by cell--cell contact and cAMP. Cell. 1981 Jun;24(3):785–797. doi: 10.1016/0092-8674(81)90104-5. [DOI] [PubMed] [Google Scholar]
  6. Elder R. T., St John T. P., Stinchcomb D. T., Davis R. W., Scherer S., Davis R. W. Studies on the transposable element Ty1 of yeast. I. RNA homologous to Ty1. II. Recombination and expression of Ty1 and adjacent sequences. Cold Spring Harb Symp Quant Biol. 1981;45(Pt 2):581–591. doi: 10.1101/sqb.1981.045.01.075. [DOI] [PubMed] [Google Scholar]
  7. Firtel R. A., Cockburn A., Frankel G., Hershfield V. Structural organization of the genome of Dictyostelium discoideum: analysis by EcoR1 restriction endonuclease. J Mol Biol. 1976 Apr 25;102(4):831–852. doi: 10.1016/0022-2836(76)90294-1. [DOI] [PubMed] [Google Scholar]
  8. Georgiev G. P., Ilyin Y. V., Chmeliauskaite V. G., Ryskov A. P., Kramerov D. A., Skryabin K. G., Krayev A. S., Lukanidin E. M., Grigoryan M. S. Mobile dispersed genetic elements and other middle repetitive DNA sequences in the genomes of Drosophila and mouse: transcription and biological significance. Cold Spring Harb Symp Quant Biol. 1981;45(Pt 2):641–654. doi: 10.1101/sqb.1981.045.01.082. [DOI] [PubMed] [Google Scholar]
  9. Mangiarotti G., Chung S., Zuker C., Lodish H. F. Selection and analysis of cloned developmentally-regulated Dictyostelium discoideum genes by hybridization-competition. Nucleic Acids Res. 1981 Feb 25;9(4):947–963. doi: 10.1093/nar/9.4.947. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. O'Day D. H., Lewis K. E. Diffusible mating-type factors induce macrocyst development in Dictyostelium discoideum. Nature. 1975 Apr 3;254(5499):431–432. doi: 10.1038/254431a0. [DOI] [PubMed] [Google Scholar]
  11. Potter S. S., Brorein W. J., Jr, Dunsmuir P., Rubin G. M. Transposition of elements of the 412, copia and 297 dispersed repeated gene families in Drosophila. Cell. 1979 Jun;17(2):415–427. doi: 10.1016/0092-8674(79)90168-5. [DOI] [PubMed] [Google Scholar]
  12. Rimm D. L., Horness D., Kucera J., Blattner F. R. Construction of coliphage lambda Charon vectors with BamHI cloning sites. Gene. 1980 Dec;12(3-4):301–309. doi: 10.1016/0378-1119(80)90113-4. [DOI] [PubMed] [Google Scholar]
  13. Rubin G. M., Brorein W. J., Jr, Dunsmuir P., Flavell A. J., Levis R., Strobel E., Toole J. J., Young E. Copia-like transposable elements in the Drosophila genome. Cold Spring Harb Symp Quant Biol. 1981;45(Pt 2):619–628. doi: 10.1101/sqb.1981.045.01.080. [DOI] [PubMed] [Google Scholar]
  14. Shimotohno K., Mizutani S., Temin H. M. Sequence of retrovirus provirus resembles that of bacterial transposable elements. Nature. 1980 Jun 19;285(5766):550–554. doi: 10.1038/285550a0. [DOI] [PubMed] [Google Scholar]
  15. Tchurikov N. A., Ilyin Y. V., Skryabin K. G., Ananiev E. V., Bayev A. A., Jr, Krayev A. S., Zelentsova E. S., Kulguskin V. V., Lyubomirskaya N. V., Georgiev G. P. General properties of mobile dispersed genetic elements in Drosophila melanogaster. Cold Spring Harb Symp Quant Biol. 1981;45(Pt 2):655–665. doi: 10.1101/sqb.1981.045.01.083. [DOI] [PubMed] [Google Scholar]
  16. Zuker C., Lodish H. F. Repetitive DNA sequences cotranscribed with developmentally regulated Dictyostelium discoideum mRNAs. Proc Natl Acad Sci U S A. 1981 Sep;78(9):5386–5390. doi: 10.1073/pnas.78.9.5386. [DOI] [PMC free article] [PubMed] [Google Scholar]

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