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. 1983 Aug 11;11(15):5037–5044. doi: 10.1093/nar/11.15.5037

Inverted terminal repetitions of the two linear DNA associated with the killer character of the yeast Kluyveromyces lactis.

F Sor, M Wésolowski, H Fukuhara
PMCID: PMC326235  PMID: 6878039

Abstract

The killer character of some Kluyveromyces lactis strains is associated with the presence of two linear double-stranded DNA, pGKl-1 (or k1) and pGKl-2 (or k2). Nucleotide sequencing has revealed that each DNA has inverted terminal repetitions of about 200 base-pairs whose 5' ends seem to be blocked. The repetitions of the two DNA do not share extensive sequence homology. The role of these repetitions in the replication of killer DNA is discussed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Arnberg A. C., Van Bruggen E. F., Brost P., Clegg R. A., Schutgens R. B., Weijers P. J., Goldbach R. W. Mitochondrial DNA of Tetrahymena pyriformis strain ST contains a long terminal duplication-inversion. Biochim Biophys Acta. 1975 Apr 2;383(4):359–369. doi: 10.1016/0005-2787(75)90305-6. [DOI] [PubMed] [Google Scholar]
  2. Bateman A. J. Letter: Simplification of palindromic telomere theory. Nature. 1975 Jan 31;253(5490):379–380. doi: 10.1038/253379a0. [DOI] [PubMed] [Google Scholar]
  3. Blackburn E. H., Gall J. G. A tandemly repeated sequence at the termini of the extrachromosomal ribosomal RNA genes in Tetrahymena. J Mol Biol. 1978 Mar 25;120(1):33–53. doi: 10.1016/0022-2836(78)90294-2. [DOI] [PubMed] [Google Scholar]
  4. Carusi E. A. Evidence for blocked 5'-termini in human adenovirus DNA. Virology. 1977 Jan;76(1):380–394. doi: 10.1016/0042-6822(77)90310-5. [DOI] [PubMed] [Google Scholar]
  5. Cavalier-Smith T. Palindromic base sequences and replication of eukaryote chromosome ends. Nature. 1974 Aug 9;250(5466):467–470. doi: 10.1038/250467a0. [DOI] [PubMed] [Google Scholar]
  6. Cockburn A. F., Newkirk M. J., Firtel R. A. Organization of the ribosomal RNA genes of Dictyostelium discoideum: mapping of the nontranscribed spacer regions. Cell. 1976 Dec;9(4 Pt 1):605–613. doi: 10.1016/0092-8674(76)90043-x. [DOI] [PubMed] [Google Scholar]
  7. Emery H. S., Weiner A. M. An irregular satellite sequence is found at the termini of the linear extrachromosomal rDNA in Dictyostelium discoideum. Cell. 1981 Nov;26(3 Pt 1):411–419. doi: 10.1016/0092-8674(81)90210-5. [DOI] [PubMed] [Google Scholar]
  8. Ferrero I., Rossi C., Landini M. P., Puglisi P. P. Role of the mitochondrial protein synthesis is the catabolite repression of the petite-negative yeast K.lactis. Biochem Biophys Res Commun. 1978 Jan 30;80(2):340–348. doi: 10.1016/0006-291x(78)90682-4. [DOI] [PubMed] [Google Scholar]
  9. Garon C. F., Berry K. W., Rose J. A. A unique form of terminal redundancy in adenovirus DNA molecules. Proc Natl Acad Sci U S A. 1972 Sep;69(9):2391–2395. doi: 10.1073/pnas.69.9.2391. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Gunge N., Murata K., Sakaguchi K. Transformation of Saccharomyces cerevisiae with linear DNA killer plasmids from Kluyveromyces lactis. J Bacteriol. 1982 Jul;151(1):462–464. doi: 10.1128/jb.151.1.462-464.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Gunge N., Sakaguchi K. Intergeneric transfer of deoxyribonucleic acid killer plasmids, pGKl1 and pGKl2, from Kluyveromyces lactis into Saccharomyces cerevisiae by cell fusion. J Bacteriol. 1981 Jul;147(1):155–160. doi: 10.1128/jb.147.1.155-160.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Gunge N., Tamaru A., Ozawa F., Sakaguchi K. Isolation and characterization of linear deoxyribonucleic acid plasmids from Kluyveromyces lactis and the plasmid-associated killer character. J Bacteriol. 1981 Jan;145(1):382–390. doi: 10.1128/jb.145.1.382-390.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Katzen A. L., Cann G. M., Blackburn E. H. Sequence-specific fragmentation of macronuclear DNA in a holotrichous ciliate. Cell. 1981 May;24(2):313–320. doi: 10.1016/0092-8674(81)90321-4. [DOI] [PubMed] [Google Scholar]
  14. Kemble R. J., Thompson R. D. S1 and S2, the linear mitochondrial DNAs present in a male sterile line of maize, possess terminally attached proteins. Nucleic Acids Res. 1982 Dec 20;10(24):8181–8190. doi: 10.1093/nar/10.24.8181. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Klobutcher L. A., Swanton M. T., Donini P., Prescott D. M. All gene-sized DNA molecules in four species of hypotrichs have the same terminal sequence and an unusual 3' terminus. Proc Natl Acad Sci U S A. 1981 May;78(5):3015–3019. doi: 10.1073/pnas.78.5.3015. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Lawn R. M. Gene-sized DNA molecules of the Oxytricha macronucleus have the same terminal sequence. Proc Natl Acad Sci U S A. 1977 Oct;74(10):4325–4328. doi: 10.1073/pnas.74.10.4325. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Maxam A. M., Gilbert W. Sequencing end-labeled DNA with base-specific chemical cleavages. Methods Enzymol. 1980;65(1):499–560. doi: 10.1016/s0076-6879(80)65059-9. [DOI] [PubMed] [Google Scholar]
  18. Niwa O., Sakaguchi K., Gunge N. Curing of the killer deoxyribonucleic acid plasmids of Kluyveromyces lactis. J Bacteriol. 1981 Dec;148(3):988–990. doi: 10.1128/jb.148.3.988-990.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Oka Y., Shiota S., Nakai S., Nishida Y., Okubo S. Inverted terminal repeat sequence in the macronuclear DNA of Stylonychia pustulata. Gene. 1980 Sep;10(4):301–306. doi: 10.1016/0378-1119(80)90150-x. [DOI] [PubMed] [Google Scholar]
  20. Pluta A. F., Kaine B. P., Spear B. B. The terminal organization of macronuclear DNA in Oxytricha fallax. Nucleic Acids Res. 1982 Dec 20;10(24):8145–8154. doi: 10.1093/nar/10.24.8145. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Rekosh D. M., Russell W. C., Bellet A. J., Robinson A. J. Identification of a protein linked to the ends of adenovirus DNA. Cell. 1977 Jun;11(2):283–295. doi: 10.1016/0092-8674(77)90045-9. [DOI] [PubMed] [Google Scholar]
  22. Walmsley R. M., Szostak J. W., Petes T. D. Is there left-handed DNA at the ends of yeast chromosomes? Nature. 1983 Mar 3;302(5903):84–86. doi: 10.1038/302084a0. [DOI] [PubMed] [Google Scholar]
  23. Wesley R. D. Inverted repetitious sequences in the macronuclear DNA of hypotrichous ciliates. Proc Natl Acad Sci U S A. 1975 Feb;72(2):678–682. doi: 10.1073/pnas.72.2.678. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Wesolowski M., Fukuhara H. Linear mitochondrial deoxyribonucleic acid from the yeast Hansenula mrakii. Mol Cell Biol. 1981 May;1(5):387–393. doi: 10.1128/mcb.1.5.387. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Wimmer E. Genome-linked proteins of viruses. Cell. 1982 Feb;28(2):199–201. doi: 10.1016/0092-8674(82)90335-x. [DOI] [PubMed] [Google Scholar]
  26. Wolfson J., Dressler D. Adenovirus-2 DNA contains an inverted terminal repetition. Proc Natl Acad Sci U S A. 1972 Oct;69(10):3054–3057. doi: 10.1073/pnas.69.10.3054. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Yao M. C., Yao C. H. Repeated hexanucleotide C-C-C-C-A-A is present near free ends of macronuclear DNA of Tetrahymena. Proc Natl Acad Sci U S A. 1981 Dec;78(12):7436–7439. doi: 10.1073/pnas.78.12.7436. [DOI] [PMC free article] [PubMed] [Google Scholar]

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