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. 1983 Aug 25;11(16):5775–5791. doi: 10.1093/nar/11.16.5775

Maintenance and incompatibility of plasmids carrying the replication origin of the Escherichia coli chromosome: evidence for a control region of replication between oriC and asnA.

A R Stuitje, M Meijer
PMCID: PMC326313  PMID: 6310513

Abstract

Plasmids that replicate only by means of the cloned Escherichia coli replication origin (oriC) are called minichromosomes or oriC-plasmids. In this paper it is shown that sequences located between oriC and asnA are involved in maintenance and incompatibility of minichromosomes. These sequences include part of the 16kD and 17kD genes, previously allocated within this region (1,2). Transcription towards oriC that is initiated at the 16kD promoter, specifically enhances the stability and copy-number of minichromosomes. Three regions are involved in minichromosome incompatibility. One region, incA, includes the minimal oriC sequence. A second, incB, maps within a 210 base pairs fragment that overlaps the 16kD promoter. The third, incC, encompasses the 17kD gene. Neither one of the regions expresses incompatibility on its own, but the additional presence of one of the others is required. The data presented indicate that sequences of the 16kD and 17kD genes are part of the replication control system of oriC-plasmids.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Andrés I., Slocombe P. M., Cabello F., Timmis J. K., Lurz R., Burkardt H. J., Timmis K. N. Plasmid replication functions. II. Cloning analysis of the repA replication region of antibiotic resistance plasmid R6-5. Mol Gen Genet. 1979 Jan 5;168(1):1–25. doi: 10.1007/BF00267929. [DOI] [PubMed] [Google Scholar]
  2. Birnboim H. C., Doly J. A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Res. 1979 Nov 24;7(6):1513–1523. doi: 10.1093/nar/7.6.1513. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Cleary J. M., Smith D. W., Harding N. E., Zyskind J. W. Primary structure of the chromosomal origins (oriC) of Enterobacter aerogenes and Klebsiella pneumoniae: comparisons and evolutionary relationships. J Bacteriol. 1982 Jun;150(3):1467–1471. doi: 10.1128/jb.150.3.1467-1471.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Cohen S. N., Chang A. C., Hsu L. Nonchromosomal antibiotic resistance in bacteria: genetic transformation of Escherichia coli by R-factor DNA. Proc Natl Acad Sci U S A. 1972 Aug;69(8):2110–2114. doi: 10.1073/pnas.69.8.2110. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Fuller R. S., Kaguni J. M., Kornberg A. Enzymatic replication of the origin of the Escherichia coli chromosome. Proc Natl Acad Sci U S A. 1981 Dec;78(12):7370–7374. doi: 10.1073/pnas.78.12.7370. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Gottesman M. E., Yarmolinsky M. B. Integration-negative mutants of bacteriophage lambda. J Mol Biol. 1968 Feb 14;31(3):487–505. doi: 10.1016/0022-2836(68)90423-3. [DOI] [PubMed] [Google Scholar]
  7. Hansen F. G., Hansen E. B., Atlung T. The nucleotide sequence of the dnaA gene promoter and of the adjacent rpmH gene, coding for the ribosomal protein L34, of Escherichia coli. EMBO J. 1982;1(9):1043–1048. doi: 10.1002/j.1460-2075.1982.tb01294.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Hobom G. Replication signals in prokaryotic DNA. Curr Top Microbiol Immunol. 1981;94-95:93–142. doi: 10.1007/978-3-642-68120-2_3. [DOI] [PubMed] [Google Scholar]
  9. Holmes D. S., Quigley M. A rapid boiling method for the preparation of bacterial plasmids. Anal Biochem. 1981 Jun;114(1):193–197. doi: 10.1016/0003-2697(81)90473-5. [DOI] [PubMed] [Google Scholar]
  10. Lother H., Messer W. Promoters in the E. coli replication origin. Nature. 1981 Nov 26;294(5839):376–378. doi: 10.1038/294376a0. [DOI] [PubMed] [Google Scholar]
  11. Maxam A. M., Gilbert W. Sequencing end-labeled DNA with base-specific chemical cleavages. Methods Enzymol. 1980;65(1):499–560. doi: 10.1016/s0076-6879(80)65059-9. [DOI] [PubMed] [Google Scholar]
  12. Meijer M., Beck E., Hansen F. G., Bergmans H. E., Messer W., von Meyenburg K., Schaller H. Nucleotide sequence of the origin of replication of the Escherichia coli K-12 chromosome. Proc Natl Acad Sci U S A. 1979 Feb;76(2):580–584. doi: 10.1073/pnas.76.2.580. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Meijer M., Messer W. Functional analysis of minichromosome replication: bidirectional and unidirectional replication from the Escherichia coli replication origin, oriC. J Bacteriol. 1980 Aug;143(2):1049–1053. doi: 10.1128/jb.143.2.1049-1053.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Messer W., Bergmans H. E., Meijer M., Womack J. E., Hansen F. G., von Meyenburg K. Mini-chromosomes: plasmids which carry the E. coli replication origin. Mol Gen Genet. 1978 Jul 4;162(3):269–275. doi: 10.1007/BF00268852. [DOI] [PubMed] [Google Scholar]
  15. Messer W., Meijer M., Bergmans H. E., Hansen F. G., von Meyenburg K., Beck E., Schaller H. Origin of replication, oriC, of the Escherichia coli K12 chromosome: nucleotide sequence. Cold Spring Harb Symp Quant Biol. 1979;43(Pt 1):139–145. doi: 10.1101/sqb.1979.043.01.020. [DOI] [PubMed] [Google Scholar]
  16. Morelli G., Buhk H. J., Fisseau C., Lother H., Yoshinaga K., Messer W. Promoters in the region of the E. coli replication origin. Mol Gen Genet. 1981;184(2):255–259. doi: 10.1007/BF00272913. [DOI] [PubMed] [Google Scholar]
  17. Ogawa H., Shimada K., Tomizawa J. Studies on radiation-sensitive mutants of E. coli. I. Mutants defective in the repair synthesis. Mol Gen Genet. 1968 May 3;101(3):227–244. doi: 10.1007/BF00271625. [DOI] [PubMed] [Google Scholar]
  18. Ogura T., Miki T., Hiraga S. Copy-number mutants of the plasmid carrying the replication origin of the Escherichia coli chromosome: evidence for a control region of replication. Proc Natl Acad Sci U S A. 1980 Jul;77(7):3993–3997. doi: 10.1073/pnas.77.7.3993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Oka A., Sugimoto K., Takanami M., Hirota Y. Replication origin of the Escherichia coli K-12 chromosome: the size and structure of the minimum DNA segment carrying the information for autonomous replication. Mol Gen Genet. 1980 Apr;178(1):9–20. doi: 10.1007/BF00267207. [DOI] [PubMed] [Google Scholar]
  20. Stuitje A. R., Spelt C. E., Veltkamp E., Nijkamp H. J. Identification of mutations affecting replication control of plasmid Clo DF13. Nature. 1981 Mar 19;290(5803):264–267. doi: 10.1038/290264a0. [DOI] [PubMed] [Google Scholar]
  21. Sugimoto K., Oka A., Sugisaki H., Takanami M., Nishimura A., Yasuda Y., Hirota Y. Nucleotide sequence of Escherichia coli K-12 replication origin. Proc Natl Acad Sci U S A. 1979 Feb;76(2):575–579. doi: 10.1073/pnas.76.2.575. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Sutcliffe J. G. Complete nucleotide sequence of the Escherichia coli plasmid pBR322. Cold Spring Harb Symp Quant Biol. 1979;43(Pt 1):77–90. doi: 10.1101/sqb.1979.043.01.013. [DOI] [PubMed] [Google Scholar]
  23. Twigg A. J., Sherratt D. Trans-complementable copy-number mutants of plasmid ColE1. Nature. 1980 Jan 10;283(5743):216–218. doi: 10.1038/283216a0. [DOI] [PubMed] [Google Scholar]
  24. Yamaguchi K., Yamaguchi M., Tomizawa J. Incompatibility of plasmids containing the replication origin of the Escherichia coli chromosome. Proc Natl Acad Sci U S A. 1982 Sep;79(17):5347–5351. doi: 10.1073/pnas.79.17.5347. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Yasuda S., Hirota Y. Cloning and mapping of the replication origin of Escherichia coli. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5458–5462. doi: 10.1073/pnas.74.12.5458. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Zyskind J. W., Deen L. T., Smith D. W. Isolation and mapping of plasmids containing the Salmonella typhimurium origin of DNA replication. Proc Natl Acad Sci U S A. 1979 Jul;76(7):3097–3101. doi: 10.1073/pnas.76.7.3097. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. von Meyenburg K., Hansen F. G., Nielsin L. D., Riise E. Origin of replication, oriC, or the Escherichia coli chromosome on specialized transducing phages lambda asn. Mol Gen Genet. 1978 Apr 17;160(3):287–295. doi: 10.1007/BF00332972. [DOI] [PubMed] [Google Scholar]
  28. von Meyenburg K., Hansen F. G., Riise E., Bergmans H. E., Meijer M., Messer W. Origin of replication, oriC, of the Escherichia coli K12 chromosome: genetic mapping and minichromosome replication. Cold Spring Harb Symp Quant Biol. 1979;43(Pt 1):121–128. doi: 10.1101/sqb.1979.043.01.018. [DOI] [PubMed] [Google Scholar]

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