Skip to main content
PMC home page
Nucleic Acids Research logoLink to Nucleic Acids Research
. 1983 Sep 24;11(18):6157–6166. doi: 10.1093/nar/11.18.6157

Nucleotide sequence of the promoter region of the E. coli lysC gene.

M Cassan, J Ronceray, J C Patte
PMCID: PMC326364  PMID: 6312411

Abstract

The regulatory region of the lysC gene (that encodes the lysine-sensitive aspartokinase of Escherichia coli) has been identified and purified by the use of lysC-lacZ fusions. Its regulatory sequence has been determined. No signals similar to those described in the case of an attenuation mechanism could be found in the long leader sequence existing between the starts of transcription and of translation.

Full text

PDF
6164

Images in this article

6164Image
on p.6164

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Barry G., Squires C., Squires C. L. Attenuation and processing of RNA from the rplJL--rpoBC transcription unit of Escherichia coli. Proc Natl Acad Sci U S A. 1980 Jun;77(6):3331–3335. doi: 10.1073/pnas.77.6.3331. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Berkner K. L., Folk W. R. Polynucleotide kinase exchange reaction: quantitave assay for restriction endonuclease-generated 5'-phosphoroyl termini in DNA. J Biol Chem. 1977 May 25;252(10):3176–3184. [PubMed] [Google Scholar]
  3. Birnboim H. C., Doly J. A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Res. 1979 Nov 24;7(6):1513–1523. doi: 10.1093/nar/7.6.1513. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Boy E., Borne F., Patte J. C. Isolation and identification of mutants constitutive for aspartokinase III synthesis in Escherichia coli K 12. Biochimie. 1979;61(10):1151–1160. doi: 10.1016/s0300-9084(80)80228-8. [DOI] [PubMed] [Google Scholar]
  5. Boy E., Patte J. C. Multivalent repression of aspartic semialdehyde dehydrogenase in Escherichia coli K-12. J Bacteriol. 1972 Oct;112(1):84–92. doi: 10.1128/jb.112.1.84-92.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Casadaban M. J., Chou J., Cohen S. N. In vitro gene fusions that join an enzymatically active beta-galactosidase segment to amino-terminal fragments of exogenous proteins: Escherichia coli plasmid vectors for the detection and cloning of translational initiation signals. J Bacteriol. 1980 Aug;143(2):971–980. doi: 10.1128/jb.143.2.971-980.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Casadaban M. J. Transposition and fusion of the lac genes to selected promoters in Escherichia coli using bacteriophage lambda and Mu. J Mol Biol. 1976 Jul 5;104(3):541–555. doi: 10.1016/0022-2836(76)90119-4. [DOI] [PubMed] [Google Scholar]
  8. Chenais J., Richaud C., Ronceray J., Cherest H., Surdin-Kerjan Y., Patte J. C. Construction of hybrid plasmids containing the lysA gene of Escherichia coli: studies of expression in Escherichia coli and Saccharomyces cerevisiae. Mol Gen Genet. 1981;182(3):456–461. doi: 10.1007/BF00293935. [DOI] [PubMed] [Google Scholar]
  9. Clément J. M., Hofnung M. Gene sequence of the lambda receptor, an outer membrane protein of E. coli K12. Cell. 1981 Dec;27(3 Pt 2):507–514. doi: 10.1016/0092-8674(81)90392-5. [DOI] [PubMed] [Google Scholar]
  10. Crawford I. P., Nichols B. P., Yanofsky C. Nucleotide sequence of the trpB gene in Escherichia coli and Salmonella typhimurium. J Mol Biol. 1980 Oct 5;142(4):489–502. doi: 10.1016/0022-2836(80)90259-4. [DOI] [PubMed] [Google Scholar]
  11. Débarbouillé M., Raibaud O. Expression of the Escherichia coli malPQ operon remains unaffected after drastic alteration of its promoter. J Bacteriol. 1983 Mar;153(3):1221–1227. doi: 10.1128/jb.153.3.1221-1227.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Haziza C., Stragier P., Patte J. C. Nucleotide sequence of the asd gene of Escherichia coli: absence of a typical attenuation signal. EMBO J. 1982;1(3):379–384. doi: 10.1002/j.1460-2075.1982.tb01178.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Humphreys G. O., Willshaw G. A., Anderson E. S. A simple method for the preparation of large quantities of pure plasmid DNA. Biochim Biophys Acta. 1975 Apr 2;383(4):457–463. doi: 10.1016/0005-2787(75)90318-4. [DOI] [PubMed] [Google Scholar]
  14. Maxam A. M., Gilbert W. Sequencing end-labeled DNA with base-specific chemical cleavages. Methods Enzymol. 1980;65(1):499–560. doi: 10.1016/s0076-6879(80)65059-9. [DOI] [PubMed] [Google Scholar]
  15. Patte J. C., Morand P., Boy E., Richaud C., Borne F. The relA locus and the regulation of lysine biosynthesis in Escherichia coli. Mol Gen Genet. 1980;179(2):319–325. doi: 10.1007/BF00425459. [DOI] [PubMed] [Google Scholar]
  16. Richaud C., Mazat J. P., Gros C., Patte J. C. Subunit structure of aspartokinase 3 of Escherichia coli K12. Eur J Biochem. 1973 Dec 17;40(2):619–629. doi: 10.1111/j.1432-1033.1973.tb03234.x. [DOI] [PubMed] [Google Scholar]
  17. Richaud F., Phuc N. H., Cassan M., Patte J. C. Regulation of aspartokinase III synthesis in Escherichia coli: isolation of mutants containing lysC-lac fusions. J Bacteriol. 1980 Jul;143(1):513–515. doi: 10.1128/jb.143.1.513-515.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Rosenberg M., Court D. Regulatory sequences involved in the promotion and termination of RNA transcription. Annu Rev Genet. 1979;13:319–353. doi: 10.1146/annurev.ge.13.120179.001535. [DOI] [PubMed] [Google Scholar]
  19. Shine J., Dalgarno L. The 3'-terminal sequence of Escherichia coli 16S ribosomal RNA: complementarity to nonsense triplets and ribosome binding sites. Proc Natl Acad Sci U S A. 1974 Apr;71(4):1342–1346. doi: 10.1073/pnas.71.4.1342. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Thèze J., Margarita D., Cohen G. N., Borne F., Patte J. C. Mapping of the structural genes of the three aspartokinases and of the two homoserine dehydrogenases of Escherichia coli K-12. J Bacteriol. 1974 Jan;117(1):133–143. doi: 10.1128/jb.117.1.133-143.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Wallace R. G., Lee N., Fowler A. V. The araC gene of Escherichia coli: transcriptional and translational start-points and complete nucleotide sequence. Gene. 1980 Dec;12(3-4):179–190. doi: 10.1016/0378-1119(80)90100-6. [DOI] [PubMed] [Google Scholar]
  22. Zurawski G., Gunsalus R. P., Brown K. D., Yanofsky C. Structure and regulation of aroH, the structural gene for the tryptophan-repressible 3-deoxy-D-arabino-heptulosonic acid-7-phosphate synthetase of Escherichia coli. J Mol Biol. 1981 Jan 5;145(1):47–73. doi: 10.1016/0022-2836(81)90334-x. [DOI] [PubMed] [Google Scholar]

Articles from Nucleic Acids Research are provided here courtesy of Oxford University Press

RESOURCES